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Molecular Biology Commons

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Zoology and genetics

Biochemistry

1992

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Full-Text Articles in Molecular Biology

Deletion Analysis Of The Starch Binding Domain From Aspergillus Glucoamylase , Luojing Chen Jan 1992

Deletion Analysis Of The Starch Binding Domain From Aspergillus Glucoamylase , Luojing Chen

Retrospective Theses and Dissertations

Glucoamylase (GA) from Aspergillus (EC 3.2.1.3) exists in two forms: GAI (amino acids 1-616) and GAII (amino acids 1-512). GAII is identical to GAI but lacks the C-terminal region (amino acids 513-616). While both forms of glucoamylase are able to digest soluble starch, only GAI has the ability to bind to and therefore hydrolyze native starch granules. To further examine the location of the starch binding domain at the C-terminus of GAI and to determine how many amino acid residues of GAI are required for starch binding, six [beta]-galactosidase fusion proteins were constructed. Affinity purified fusion ...