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Zoology and genetics

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Full-Text Articles in Molecular Biology

Genetic And Biochemical Analysis Of Aspergillus Awamori Glucoamylase Thermostability , Martin John Allen Jan 1997

Genetic And Biochemical Analysis Of Aspergillus Awamori Glucoamylase Thermostability , Martin John Allen

Retrospective Theses and Dissertations

Aspergillus awamori glucoamylase (GA) is an enzyme involved in industrial corn starch processing. Genetic and biochemical approaches were used to study the mechanisms governing GA thermostability. Three proline substitution (Xaa → Pro) mutations were constructed that were predicted to increase the enzyme's stability by decreasing its conformational entropy of unfolding. When expressed in Saccharomyces cerevisiae Ser30 → Pro increased, Asp345 → Pro did not alter and Glu408 → Pro greatly decreased GA stability as measured by resistance to irreversible thermoinactivation relative to the wild-type enzyme. The Ser30 → Pro mutation was combined with other previously identified stabilizing mutations to examine whether combining such mutations ...


Soybean Cultivar Development: A Genome Perspective , Lisa Lou Lorenzen Jan 1994

Soybean Cultivar Development: A Genome Perspective , Lisa Lou Lorenzen

Retrospective Theses and Dissertations

The purpose of this dissertation was to retrospectively analyze the process of soybean cultivar development from a molecular perspective, by following the inheritance of RFLP markers in a pedigree analysis. Five methods of analysis are included in this dissertation;Sixty-four plant introductions, cultivars, and breeding lines were analyzed to define a core set of 97 probes with a high likelihood of detecting a polymorphism in elite germplasm. The ability to trace regions of chromosomes from parent to offspring and the application of pedigree-based mapping was demonstrated, which is the cosegregation of phenotypic traits and RFLP markers based on pedigree information ...