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Full-Text Articles in Molecular Biology
The Complex Role Of Sequence And Structure In The Stability And Function Of The Tim Barrel Proteins, Yvonne H. Chan
GSBS Dissertations and Theses
Sequence divergence of orthologous proteins enables adaptation to a plethora of environmental stresses and promotes evolution of novel functions. As one of the most common motifs in biology capable of diverse enzymatic functions, the TIM barrel represents an ideal model system for mapping the phenotypic manifestations of protein sequence. Limits on evolution imposed by constraints on sequence and structure were investigated using a model TIM barrel protein, indole-3-glycerol phosphate synthase (IGPS). Exploration of fitness landscapes of phylogenetically distant orthologs provides a strategy for elucidating the complex interrelationship in the context of a protein fold.
Fitness effects of point mutations in ...