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Full-Text Articles in Molecular Biology

Gene Expression Response In Early Developmental Stages Of Rainbow Trout Exposed To Ecologically Relevant Concentrations Of Malathion, Susan Miller Dec 2012

Gene Expression Response In Early Developmental Stages Of Rainbow Trout Exposed To Ecologically Relevant Concentrations Of Malathion, Susan Miller

Theses and Dissertations

Understanding the early life stage toxic effects of environmental organophosphate exposure on organism health is crucial to identifying biomarkers that can be used for preventative care. Malathion, a potent organophosphate, is one of the most widely used organophosphates in agriculture and pest eradication. Due to its widespread use, pesticide runoff into area bodies of water poses a great threat to aquatic life and human inhabitants. Acute exposure to high concentrations of malathion causes neurological abnormalities and can result in respiratory failure, muscle spasms, and mental confusion in humans.

In the present study, the effects of malathion are observed following acute, …


Assessment Of Dim Mutants Of The Vibrio Harveyi Species Isolated From The Gulf Of Mexico, Elizabeth Bolton Aug 2012

Assessment Of Dim Mutants Of The Vibrio Harveyi Species Isolated From The Gulf Of Mexico, Elizabeth Bolton

Theses and Dissertations

Isolating environmental dim and dark mutants of V. harveyi gives insight into the positive selection and maintenance of lux genes in a natural setting. Mutants were isolated from Boca Ciega Bay, FL., on the basis of the presence of lux genes without visible luminescence when plated on SWC agar. Of 600 isolates, four mutants were found to glow minimally on X-ray film. Of these four, luminescence was rescued in three isolates to a varying degree by addition of aldehyde, a component of the bacterial luminescence reaction. These four lux operons were sequenced, and two were shown to have a frameshift …