Open Access. Powered by Scholars. Published by Universities.®

Molecular Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

1990

Iowa State University

Microbiology

Articles 1 - 1 of 1

Full-Text Articles in Molecular Biology

Isolation Of A Chicken Cardiac Muscle Titin Cdna Probe , Greg Robert Sater Jan 1990

Isolation Of A Chicken Cardiac Muscle Titin Cdna Probe , Greg Robert Sater

Retrospective Theses and Dissertations

The objective of this project was to construct and identify a cDNA probe for the striated muscle protein titin. RNA was isolated from skeletal and cardiac muscle of 16 day chicken embryos using a guanidinium isothiocyanate procedure. The RNA thus isolated was both intact (as shown by agarose gel electrophoresis) and transcribable in vitro; additionally, it was possible to obtain very large RNA molecules in this manner. cDNA was then constructed utilizing random oligodeoxynucleotides for priming of the first strand synthesis reaction, instead of the more common oligo-dT priming. Random priming was also utilized for second strand synthesis. As with ...