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Molecular Biology Commons

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1990

Iowa State University

Biophysics

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Full-Text Articles in Molecular Biology

The Crystal Structure Of Adenylosuccinate Synthetase From Escherichia Coli , Kyung Hyun Kim Jan 1990

The Crystal Structure Of Adenylosuccinate Synthetase From Escherichia Coli , Kyung Hyun Kim

Retrospective Theses and Dissertations

The crystal structure of adenylosuccinate synthetase from Eschericha coli has been pursued to a resolution of 3.7 A. Using isomorphous and anomalous (SIRAS) phases from a HgI[subscript]4[superscript]2- derivative, electron density maps were generated that clearly revealed the envelope of the enzyme dimer and the two-fold molecular symmetry of the dimer;Since the enzyme is active as a dimer consisting of two identical monomers, the method of density averaging (Bricogne, 1976) was employed to improve the electron density map based on SIRAS phases. The non-crystallographic two-fold operator, which relates two monomers, was refined against an SIRAS ...