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Full-Text Articles in Molecular Biology

Nucleotide Sequence And Further Characterization Of The Synechococcus Sp. Strain Pcc 7002 Reca Gene: Complementation Of A Cyanobacterial Reca Mutation By The Escherichia Coli Reca Gene, R C. Murphy, G E. Gasparich, D A. Bryant, R D. Porter Jan 1990

Nucleotide Sequence And Further Characterization Of The Synechococcus Sp. Strain Pcc 7002 Reca Gene: Complementation Of A Cyanobacterial Reca Mutation By The Escherichia Coli Reca Gene, R C. Murphy, G E. Gasparich, D A. Bryant, R D. Porter

Gail Gasparich

The nucleotide sequence and transcript initiation site of the Synechococcus sp. strain PCC 7002 recA gene have been determined. The deduced amino acid sequence of the RecA protein of this cyanobacterium is 56% identical and 73% similar to the Escherichia coli RecA protein. Northern (RNA) blot analysis indicates that the Synechococcus strain PCC 7002 recA gene is transcribed as a monocistronic transcript 1,200 bases in length. The 5' endpoint of the recA mRNA was mapped by primer extension by using synthetic oligonucleotides of 17 and 27 nucleotides as primers. The nucleotide sequence 5' to the mapped endpoint contained sequence ...


Regulation Of Protein Expression By Transforming Growth Factor Beta And Other Growth Modulators , Frederic William Thalacker Jan 1990

Regulation Of Protein Expression By Transforming Growth Factor Beta And Other Growth Modulators , Frederic William Thalacker

Retrospective Theses and Dissertations

The main goal of this project was to understand the signal transduction pathways through which growth modulators such as TGF-[beta], PMA and retinoic acid mediate protein expression and DNA synthesis. Transforming growth factor [beta] (TGF-[beta]) induces synthesis and secretion of a 48,000 M r protein that we have found to be type I plasminogen activator inhibitor (PAI-1). TGF-[beta] induces PAI-1 in every cell line that we have studied including near term mink lung epithelial CCL64 cells, mouse embryo fibroblast AKR-2B 84A cells, African green monkey kidney epithelial BSC-1 cells, and normal rat kidney fibroblast NRK 49F ...