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Full-Text Articles in Molecular Biology

A Temperature-Sensitive Mutant Of Escherichia Coli Affected In The Alpha Subunit Of Rna Polymerase, Majid Mehrpouyan Dec 1990

A Temperature-Sensitive Mutant Of Escherichia Coli Affected In The Alpha Subunit Of Rna Polymerase, Majid Mehrpouyan

Electronic Theses and Dissertations

A temperature-sensitive mutant of Escherichia coli affected in the alpha subunit of RNA polymerase has been investigated. Gene mapping and complementation experiments placed the mutation to temperature-sensitivity within the alpha operon at 72 min on the bacterial chromosome. The rate of RNA synthesis in vivo and the accumulation of ribosomal RNA were significantly reduced in the mutant at 44$\sp\circ$C. The thermostability at 44$\sp\circ$C of the purified holoenzyme from mutant cells was about 20% of that of the normal enzyme. Assays with T7 DNA as a template showed that the fraction of active enzyme competent ...


Bilirubin-Albumin Binding At Varying Conditions Of Hematocrit, Sodium Benzoate Concentration, And Bilirubin/Albumin Molar Ratios, Patrick Ahrens Apr 1990

Bilirubin-Albumin Binding At Varying Conditions Of Hematocrit, Sodium Benzoate Concentration, And Bilirubin/Albumin Molar Ratios, Patrick Ahrens

Life and Environmental Sciences Undergraduate Theses

Hyperbilirubinemia, which causes jaundice, is quite common in newborns. Typically bilirubin is bound to its carrier protein albumin and very little unbound bilirubin is present in the blood. However, in cases of hyperbilirubinemia there is a marked increase in unbound bilirubin, which can be toxic. The two methods traditionally used to treat hyperbilirubinemia are exchange transfusion and phototherapy. Each of these methods have drawbacks. The Department of Chemical, Bio, and Materials Engineering at Arizona State University is currently developing an extra-corporeal hemoperfusion column that will remove the unbound bilirubin fraction from the blood of jaundiced patients. This column utilizes coated ...


Isoprenylation Is Required For The Processing Of The Lamin A Precursor, Michael Sinensky, L. A. Beck, T. J. Hosick Jan 1990

Isoprenylation Is Required For The Processing Of The Lamin A Precursor, Michael Sinensky, L. A. Beck, T. J. Hosick

Michael Sinensky

The nuclear lamina proteins, prelamin A, lamin B, and a 70-kD lamina-associated protein, are posttranslationally modified by a metabolite derived from mevalonate. This modification can be inhibited by treatment with (3-R,S)-3-fluoromevalonate, demonstrating that it is isoprenoid in nature. We have examined the association between isoprenoid metabolism and processing of the lamin A precursor in human and hamster cells. Inhibition of 3-hydroxy-3-methylglutaryl coenzyme A reductase by mevinolin (lovastatin) specifically depletes endogenous isoprenoid pools and inhibits the conversion of prelamin A to lamin A. Prelamin A processing is also blocked by mevalonate starvation of Mev-1, a CHO cell line auxotrophic ...


Isoprenylation Is Required For The Processing Of The Lamin A Precursor, Michael Sinensky, L. A. Beck, T. J. Hosick Jan 1990

Isoprenylation Is Required For The Processing Of The Lamin A Precursor, Michael Sinensky, L. A. Beck, T. J. Hosick

Faculty Publications

The nuclear lamina proteins, prelamin A, lamin B, and a 70-kD lamina-associated protein, are posttranslationally modified by a metabolite derived from mevalonate. This modification can be inhibited by treatment with (3-R,S)-3-fluoromevalonate, demonstrating that it is isoprenoid in nature. We have examined the association between isoprenoid metabolism and processing of the lamin A precursor in human and hamster cells. Inhibition of 3-hydroxy-3-methylglutaryl coenzyme A reductase by mevinolin (lovastatin) specifically depletes endogenous isoprenoid pools and inhibits the conversion of prelamin A to lamin A. Prelamin A processing is also blocked by mevalonate starvation of Mev-1, a CHO cell line auxotrophic ...


Regulation Of Protein Expression By Transforming Growth Factor Beta And Other Growth Modulators , Frederic William Thalacker Jan 1990

Regulation Of Protein Expression By Transforming Growth Factor Beta And Other Growth Modulators , Frederic William Thalacker

Retrospective Theses and Dissertations

The main goal of this project was to understand the signal transduction pathways through which growth modulators such as TGF-[beta], PMA and retinoic acid mediate protein expression and DNA synthesis. Transforming growth factor [beta] (TGF-[beta]) induces synthesis and secretion of a 48,000 M r protein that we have found to be type I plasminogen activator inhibitor (PAI-1). TGF-[beta] induces PAI-1 in every cell line that we have studied including near term mink lung epithelial CCL64 cells, mouse embryo fibroblast AKR-2B 84A cells, African green monkey kidney epithelial BSC-1 cells, and normal rat kidney fibroblast NRK 49F ...


Analysis And Characterization Of The Major Histocompatibility Complex Of Domestic Animals , Yuanxin Xu Jan 1990

Analysis And Characterization Of The Major Histocompatibility Complex Of Domestic Animals , Yuanxin Xu

Retrospective Theses and Dissertations

The major histocompatibility complex (MHC) is a family of genes coding for molecules which play important roles in the immune response as well as many other cellular processes of a vertebrate organism. The understanding of the immune system of some of the domestic animals will have both practical value and theoretical significance. The purpose of this work was to study the MHC of the chicken (B) and the swine (SLA). First, in order to study the MHC class II gene structure of the chicken, a genomic library was constructed from an inbred chicken line G-B2 (MHC haplotype B[superscript]6 ...