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Full-Text Articles in Molecular Biology

Use Of Pyrimidine Pathway To Produce Mutant Plants That Fail To Respond To Wound Induction , Lan Zhou Jan 1997

Use Of Pyrimidine Pathway To Produce Mutant Plants That Fail To Respond To Wound Induction , Lan Zhou

Retrospective Theses and Dissertations

To understand how proteinase inhibitor genes, as a part of plant defense system, are activated following wounding, we have developed a molecular genetic approach to directly select for mutants blocked in wound response in Arabidopsis thaliana. The wound-inducible promoter from the potato Proteinase Inhibitor 11 gene (pin2) was linked to a bacterial negative marker gene codA encoding for cytosine deaminase and transformed into Arabidopsis thaliana. The cytosine deaminase activity was detected in the leaves of the transgenic plants induced by sucrose, ABA, methyl jasmonate and mechanical wounding. Methyl jasmonate was the best for the pin2 induction in the seedlings among ...


Gene Expression Studies Of Murine Pregnancy Associated Granulated Metrial Gland And Decidual Cells , Melissa Phillips Allen Jan 1997

Gene Expression Studies Of Murine Pregnancy Associated Granulated Metrial Gland And Decidual Cells , Melissa Phillips Allen

Retrospective Theses and Dissertations

During pregnancy in viviparous mammals, the maternal decidua develops between the embryo and the uterine endometrium. This dissertation describes research on two different cell types present within the rodent decidua during pregnancy, namely, granulated metrial gland (GMG) cells and decidual cells;GMG cells are natural killer cells that proliferate and differentiate within the murine uterus during pregnancy. Their differentiation is associated with the accumulation of cytolytic mediators within cytoplasmic granules. The signaling mechanisms required for GMG cell differentiation, however, are largely unknown. In this study, expression of the genes coding for granzymes D, E, F, and G (granzymes D-G) was ...


Suppressor Analysis Of The Protein Kinase Elm1p, An Enzyme Involved In The Regulation Of Yeast Morphology , Nicholas Patrick Edgington Jan 1997

Suppressor Analysis Of The Protein Kinase Elm1p, An Enzyme Involved In The Regulation Of Yeast Morphology , Nicholas Patrick Edgington

Retrospective Theses and Dissertations

Saccharomyces cerevisiae is an ascomycete that has several developmental options, with concomitant morphological changes, that depend on cell type and nutrient status. In response to these signals, vegetative Saccharomyces cerevisiae cells assume one of two distinct morphologies, the yeast form or the filamentous form. Molecules affecting this morphogenetic response were identified by analyzing transposon-induced or spontaneous suppressor mutations that restore yeast form growth to a strain that constitutively exhibits filamentous form characteristics owing to mutation of the protein kinase Elm1p. One transposon-tagged mutation was isolated and is allelic to the negative regulatory subunit Bcy1p, thus activation of cAMP dependent protein ...


Structure And Function Of Escherichia Coli Valine Transfer Rna In Aminoacylation And Ternary Complex Formation , Jack Chia-Hsiang Liu Jan 1997

Structure And Function Of Escherichia Coli Valine Transfer Rna In Aminoacylation And Ternary Complex Formation , Jack Chia-Hsiang Liu

Retrospective Theses and Dissertations

The relationship of E. coli tRNA Val structure to its function in the aminoacylation reaction and the later steps of polypeptide synthesis was investigated. Steady-state kinetic studies of the aminoacylation of invitro transcribed tRNA Val variants have shown that nucleotides, A35, C36, A73, and G20, are important for the specific recognition of E. coli tRNA Val by valyl-tRNA synthetase (ValRS). Identity swap experiments, testing the efficiency of this "recognition nucleotide set" in converting yeast tRNA Phe and E. coli tRNA Phe into caline acceptors, confirm the importance of the recognition nucleotide set. In addition, a minor recognition nucleotide, G45, was ...


Studies On The Structure-Function Relationships And Regulation Mechanisms Of Escherichia Coli Adenylosuccinate Synthetase , Wenyan Wang Jan 1996

Studies On The Structure-Function Relationships And Regulation Mechanisms Of Escherichia Coli Adenylosuccinate Synthetase , Wenyan Wang

Retrospective Theses and Dissertations

Molecular biological, biochemical, and biophysical techniques were utilized to explore structure-function relationship of Escherichia coli adenylosuccinate synthetase. For the first time, residues Arg303, Arg304 and Arg305 were implicated as the determinants in the recognition and binding of substrate L-aspartate. Replacing Arg305 with leucine resulted in over a 100-fold decrease in kcat, suggesting a possible role of Arg305 in catalysis. pH dependent kinetic studies indicated that the side chain of Gln224 is not only involved in recognition of IMP, but also in stabiling the intermediate 6-phosphoryl-IMP. Substitution of the side chain amide with a carboxylate group resulted in loss of catalytic ...


Identification Of Mouse Sip24/24p3 As A New Acute Phase Protein And Study Of Its Expression , Quansheng Liu Jan 1996

Identification Of Mouse Sip24/24p3 As A New Acute Phase Protein And Study Of Its Expression , Quansheng Liu

Retrospective Theses and Dissertations

Mouse SIP24 was originally discovered as a 24 k Da inducible protein secreted from quiescent Balb/c 3T3 fibroblast cells. Based on the analysis of peptide sequencing results and Western blot studies, SIP24 was identified as identical to the mouse protein encoded by 24P3 and is thus referred to as SIP24/24P3 in this thesis. SIP24/24P3 is a member of the lipocalin protein family, and its human homolog NGAL (neutrophil gelatinase associated lipocalin) has been reported to bind the chemotactic factor, fMLP (N-formylmethionyl-leucyl-phenylalanine);Studies were carried out to test the hypothesis that SIP24/24P3 is an acute phase protein ...


X-Ray Crystallographic Studies Of The Nucleotide Binding Sites On Adenylosuccinate Synthetase From Esherichia Coli , Bradley Wayne Poland Jan 1996

X-Ray Crystallographic Studies Of The Nucleotide Binding Sites On Adenylosuccinate Synthetase From Esherichia Coli , Bradley Wayne Poland

Retrospective Theses and Dissertations

The crystal structures of unligated adenylosuccinate synthetase from Esherichia coli in the space groups P21 and P212121 have been refined to R-factors of 0.199 and 0.206 against 2.0 and 2.5A data, respectively. The dominant structural motif of the synthetase monomer is a central 10 stranded [beta]-sheet composed of 9 parallel strands and a 10th antiparallel strand. Four subdomains extend off of this [beta]-sheet and contribute loops of residues which are disordered. The dimer interface involves two nearly-independent regions. A pair of helicea (H4 & H5) interacting with their symmetry equivalent mates defines the first region. The second region involves structural elements which connect strands of the central [beta]-sheet. Residues in the putative IMP binding site are also located at or near the dimer interface;The P212121 crystal form allows gradual infusion of guanosine triphosphate ribonucleotides ...


The Structure And Function Of Human Brain Hexokinase , Chenbo Zeng Jan 1996

The Structure And Function Of Human Brain Hexokinase , Chenbo Zeng

Retrospective Theses and Dissertations

An ATP binding site model for human brain hexokinase was established based on the similarity of the ATP-binding domains among actin, heat shock protein, glycerol kinase and brain hexokinase. Site directed mutagenesis was performed on the residues in the putative ATP-binding site in order to substantiate or refute the proposed model. The k cat values decreased 1000- and 200-fold for the Asp532Lys and Asp532Glu mutants, 114- and 12-fold for the Arg539Ile and Arg539Lys mutants, 2000- and 2-fold for the Thr680Val and Thr680Ser mutants, respectively. It is suggested that these residues are important for catalysis by either keeping the [gamma]-phosphoryl ...


Study Of The Structural Requirements Of E Coli Valine Transfer Rna In Aminoacylation And Protein Synthesis , Mingsong Liu Jan 1995

Study Of The Structural Requirements Of E Coli Valine Transfer Rna In Aminoacylation And Protein Synthesis , Mingsong Liu

Retrospective Theses and Dissertations

The structural requirements for the function of the 3[superscript]' CCA end of E. coli valine transfer RNA in aminoacylation and protein synthesis have been investigated, by studying the activities of 3[superscript]' end tRNA[superscript] Val variants in these processes. These studies show that 3[superscript]' end tRNA[superscript] Val variants can be obtained which are functional in both aminoacylation and later steps of protein synthesis. Replacement of the 3[superscript]' terminal adenosine with either cytosine or uracil yields tRNAs that retain almost full aminoacylation activity (40-50% that of wild type tRNA[superscript] Val). The tRNA[superscript] Val variant ...


Biochemical And Molecular Biological Characterization Of Plant Acetyl-Coa Carboxylases , James J. Caffrey Jan 1995

Biochemical And Molecular Biological Characterization Of Plant Acetyl-Coa Carboxylases , James J. Caffrey

Retrospective Theses and Dissertations

Acetyl-CoA carboxylase is a biotin-containing enzyme which catalyzes the ATP-dependent carboxylation of acetyl-CoA to form malonyl-CoA. Plants are unique among organisms in that malonyl-CoA is utilized in both de novo fatty acid biosynthesis, and as an intermediate in a number of other plant primary and secondary metabolic pathways. Some of these metabolic pathways operate in different subcellular and cellular compartments. Therefore, plants have a mechanism to generate separate pools of malonyl-CoA. Understanding this mechanism has been the impetus for the research reported in this dissertation;Immunological and structural analyses of a 60 kDa biotinylated polypeptide (60kDBP) from carrot indicate that ...


Elm1p, A Protein Kinase That Regulates Cell Morphology In Saccharomyces Cerevisiae , Carla Marie Koehler Jan 1995

Elm1p, A Protein Kinase That Regulates Cell Morphology In Saccharomyces Cerevisiae , Carla Marie Koehler

Retrospective Theses and Dissertations

Saccharomyces cerevisiae is a pleimorphic ascomycete, capable of growing in the yeast form or the pseudohyphal form. The pseudohyphal form is characterized by elongated cells that possess a unipolar budding pattern and grow in chains away from the center of the colony. This change in morphology is induced by nitrogen starvation in the presence of carbon. A collection of 14 elm (elongated morphology) mutants previously have been isolated that grow as pseudohyphae constitutively. Of these mutants, the product of the ELM1 gene was identified and coded for a novel protein kinase. From genetic analyses, ELM1 seemingly functioned downstream of STE7 ...


The Role(S) Of Arginine-Specific Mono(Adp-Ribosyl)Transferase In Skeletal Muscle Cells , Hui-Yu Huang Jan 1995

The Role(S) Of Arginine-Specific Mono(Adp-Ribosyl)Transferase In Skeletal Muscle Cells , Hui-Yu Huang

Retrospective Theses and Dissertations

The overall goal of this research project was to examine the biological function of arginine-specific mono(ADP-ribosyl) transferase in skeletal muscle cells. To better understand the function of this enzyme in skeletal muscle, the experimental approach to accomplish this goal consisted of two specific objectives. The first objective was to identify the protein substrates for this enzyme in myogenic cell cultures and cellular fractions. Embryonic chick primary muscle cell cultures were used as a working system for this purpose. In membrane fractions of 96-h myotube cultures incubated with [[superscript]32 P]NAD under conditions selected for mono(ADP-ribosyl)ation, proteins ...


Investigation Of The Gtp-Binding Consensus Sequences In Escherichia Coli Adenylosuccinate Synthetase And The Enzyme's Reaction Mechanism , Chulhun Kang Jan 1995

Investigation Of The Gtp-Binding Consensus Sequences In Escherichia Coli Adenylosuccinate Synthetase And The Enzyme's Reaction Mechanism , Chulhun Kang

Retrospective Theses and Dissertations

In this dissertation, on the basis of the similarities in sequence and structure between GTP-binding proteins and AMPSase, it was of interest to determine whether or not the GTP-binding consensus sequences have similar functions. It will be shown that the GTP-binding consensus sequences found in AMPSase play similar roles to those of the GTPase superfamily. The kinetic results show that Lys331 interacts with GTP through hydrophobic interactions between its linear side chain and the aromatic ring of the guanine base of GTP. Also, structural characterization of the G15V mutant using circular dichroism (CD) spectrometry, NMR spectroscopy, and spectrofluorometry indicated that ...


Regulation Of Mitogen Regulated Protein/Proliferin Gene Expression In 3t3 Cells By Basic Fibroblast Growth Factor , Manzoor Ali P. K. Mohideen Jan 1994

Regulation Of Mitogen Regulated Protein/Proliferin Gene Expression In 3t3 Cells By Basic Fibroblast Growth Factor , Manzoor Ali P. K. Mohideen

Retrospective Theses and Dissertations

Mitogen regulated protein/proliferin (MRP/PLF) is a murine uterine growth factor belonging to the prolactin/growth hormone gene superfamily. Basic fibroblast growth factor (bFGF) regulates its expression in 3T3 cells and is speculated to be among its regulators in vivo. In order to gain insights into its regulation, experiments were performed to elucidate the mechanisms by which bFGF regulates mrp/plf gene expression in 3T3 cells. RT-PCR analyses demonstrated that of the three different forms of MRP/PLF proteins only one, PLF1, is expressed in these cells in response to bFGF. PLF1 could be the product of either plf42 ...


Structure And Function Of Unmodified E. Coli Valine-Trna , Dongxian Yue Jan 1994

Structure And Function Of Unmodified E. Coli Valine-Trna , Dongxian Yue

Retrospective Theses and Dissertations

The effects of nucleoside modifications on E. coli tRNA[superscript]Val structure have been probed by imino [superscript]1H NMR. The NMR data shows that the structure of in vitro transcribed (unmodified) and native (modified) tRNA[superscript] Val are very similar in 15 mM Mg[superscript]2+. Temperature dependence of the spectra reveals that nucleoside modifications stabilize tertiary interactions between T and D loops. On removal of Mg[superscript]2+, unmodified tRNA[superscript] Val undergoes remarkable structural changes which are not observed in native tRNA[superscript] Val. There is near total disruption of the D stem and of tertiary interactions ...


Genetic Analysis Of Pseudohyphal Growth In The Budding Yeast Saccharomyces Cerevisiae , Melissa Jo Blacketer Jan 1994

Genetic Analysis Of Pseudohyphal Growth In The Budding Yeast Saccharomyces Cerevisiae , Melissa Jo Blacketer

Retrospective Theses and Dissertations

The budding yeast Saccharomyces cerevisiae is a dimorphic organism that can assume either a yeast-like or pseudohyphal form. Nitrogen limitation induces pseudohyphal growth, which is characterized by branched chains of elongated cells. Pseudohyphal cells can grow invasively in agar medium, whereas yeast-like cells do not. To identify factors involved in morphologic differentiation, S. cerevisiae mutants exhibiting a constitutive cell elongation morphology were isolated. Genetic analysis identified 28 recessive and 2 semi-dominant mutations that cause abnormal morphology, and placed these in 14 distinct gene loci, termed ELM, for ELongated Morphology. Many elm mutations cause multiple aspects of pseudohyphal growth, and thus ...


Characterization Of [Beta]-Methylcrotonyl-Coa Carboxylase Of Tomato: A Newly Identified Biotin Enzyme In Plants , Xun Wang Jan 1993

Characterization Of [Beta]-Methylcrotonyl-Coa Carboxylase Of Tomato: A Newly Identified Biotin Enzyme In Plants , Xun Wang

Retrospective Theses and Dissertations

Biotin-containing enzymes catalyze critical reactions in diverse metabolic processes. In contrast to the extensive characterizations of biotin-containing enzymes from animal and microbial sources, little is known about these enzymes from plants. I have isolated and sequenced cDNA clones coding for the biotin-containing subunit [beta]-methylcrotonyl-CoA carboxylase (MCCase) from tomato, the first molecular cloning of this enzyme from any organism, and the first molecular cloning of a plant biotin-containing enzyme. Comparison of the deduced amino acid sequence from these clones with other biotin-containing enzymes suggests that the 78 kD subunit carries biotin carboxylase and biotin carboxyl carrier functions. I have shown ...


Molecular Cloning And Characterization Of 3-Methylcrotonyl-Coa Carboxylase From Soybean , Jianping Song Jan 1993

Molecular Cloning And Characterization Of 3-Methylcrotonyl-Coa Carboxylase From Soybean , Jianping Song

Retrospective Theses and Dissertations

A cDNA clone and genes coding for the 85 kDa biotin-containing polypeptide of soybean has been isolated and sequenced. This 85 kDa polypeptide has been identified as the biotin-containing subunit of 3-methylcrotonyl-CoA carboxylase (MCCase). This is the first plant gene coding for a biotin-containing enzyme to be cloned. It is also the first time that the primary sequence of the biotin-containing subunit of MCCase has been determined;The deduced amino acid sequence of the biotin-containing subunit of MCCase of soybean shows high homology with other known biotin-containing enzymes. Amino acid sequence homology between the soybean MCCase and other biotin enzymes ...


Genetic Characterization And Molecular Cloning Of Genes Involved In Kernel Development In Zea Mays , Michael John Scanlon Jan 1993

Genetic Characterization And Molecular Cloning Of Genes Involved In Kernel Development In Zea Mays , Michael John Scanlon

Retrospective Theses and Dissertations

This dissertation describes the genetic and molecular characterization of maize genes that are required for kernel development. Maize stocks in which the Robertson's Mutator (Mu) transposable element system are especially active were used to generate 75 mutations affecting kernel development. Sixty-four kernel mutations were located to chromosome arm, and 17 were placed to more precise locations on the maize genetic map. Viability tests of kernels homozygous for defective kernel (dek) mutations revealed numerous pleiotropic effects on plants obtained from mutant kernels. Fifteen putative Mutator-induced mutations tested negative for allelism to any known gene required for kernel development, and thus ...


Molecular Cloning, Expression, And Protein Interaction Of Avian Muscle Titin , Kuan Onn Tan Jan 1993

Molecular Cloning, Expression, And Protein Interaction Of Avian Muscle Titin , Kuan Onn Tan

Retrospective Theses and Dissertations

Titin (also called connectin) is a megadalton protein that constitutes up to 10% of the myofibrillar protein in skeletal and cardiac muscles. The objectives of this study were: (1) to determine a portion of the primary structure of titin; and (2) to identify interactions of cloned titin fragments with other myofibrillar proteins;A 0.2 kb cDNA clone, initially isolated by antibody screening of a chick cardiac muscle cDNA library, was used to screen a cDNA library constructed from 16-day embryonic chick cardiac muscle RNA size-selected for large RNAs. Three overlapping clones, containing 2.4 kb of titin sequence, were ...


Genetic And Biochemical Characterization Of The P21[Superscript Ras]-Related Protein Rho1p In The Yeast Saccharomyces Cerevisiae , Joni Marie Seeling Johnson Jan 1992

Genetic And Biochemical Characterization Of The P21[Superscript Ras]-Related Protein Rho1p In The Yeast Saccharomyces Cerevisiae , Joni Marie Seeling Johnson

Retrospective Theses and Dissertations

Rho1p is a guanine nucleotide binding protein coded for by the Saccharomyces cerevisiae gene RHO1, which is homologous to p21[superscript] ras and other members of the ras superfamily of small molecular weight GTP binding proteins. This protein family is highly conserved in evolution and in some instances is known to be involved in control of cell proliferation. The precise physiological functions of most p21[superscript] ras-related proteins, however, are not known. In order to gain further insight into the physiological function of rho proteins, this research addressed the genetic and biochemical characterization of Rho1p, as well as the subcellular ...


Cloning And Characterization Of Mink Plasminogen Activator Inhibitor Type 1 (Pai-1) Cdna And The Regulation Of Mink Pai-1 Expression At Mrna Level , Tsung-Hsien Chuang Jan 1992

Cloning And Characterization Of Mink Plasminogen Activator Inhibitor Type 1 (Pai-1) Cdna And The Regulation Of Mink Pai-1 Expression At Mrna Level , Tsung-Hsien Chuang

Retrospective Theses and Dissertations

Mink plasminogen activator inhibitor type 1 (PAI-1) is a glycoprotein with molecular weight about 50 kD and is highly regulated in cultured cells. In mink lung CCL64 epithelial cells, epidermal growth factor (EGF), transforming growth factor-[beta] (TGF-[beta]), and 12-O-tetradecanoyl phorbol-13-acetate (TPA) increase the synthesis of PAI-1. Epidermal growth factor and TGF-[beta] synergistically stimulate the synthesis of PAI-1. The synergistic effect and factors which govern the PAI-1 expression in mink lung CCL64 cells were studied by using a mink PAI-1 cDNA as a probe to detect PAI-1 mRNA;The mink PAI-1 cDNA was cloned from a cDNA library ...


Characterization Of The Saccharomyces Cerevisiae Gene Mgp1, A Novel Member Of The Ras Gene Superfamily That Functions In The Mitochondrial Biogenesis , Shu-Hwa Lee Tzeng Jan 1992

Characterization Of The Saccharomyces Cerevisiae Gene Mgp1, A Novel Member Of The Ras Gene Superfamily That Functions In The Mitochondrial Biogenesis , Shu-Hwa Lee Tzeng

Retrospective Theses and Dissertations

This dissertation characterizes Mgp1p, a mitochondrial protein that functions in mitochondrial biogenesis. Mgp1p is the first protein related to the p21[superscript] H-ras oncoprotein shown to function in mitochondria;The Saccharomyces cerevisiae gene MGP1 was isolated based on its ability to restore respiratory competence to a strain containing the nuclear petite mutation msg1-1. MGP1 codes for a protein of approximately 58 kDa, with an amino terminal sequence typical of a mitochondrial targeting peptide. A 190 residue region of Mgp1p shares approximately 30% amino acid sequence identity with any member of p21[superscript] ras-related protein superfamily, with particularly strong homology in ...


Fluorescence Studies And Consequences Of Amino Acid Substitutions With Escherichia Coli Adenylosuccinate Synthetase , Chandrasen Soans Jan 1992

Fluorescence Studies And Consequences Of Amino Acid Substitutions With Escherichia Coli Adenylosuccinate Synthetase , Chandrasen Soans

Retrospective Theses and Dissertations

Probes were introduced into the active site of adenylosuccinate synthetase from Escherichia coli for fluorescence energy transfer measurements and to measure enzyme conformational changes attending ligand binding. The distances from fluorescent reporter groups covalently bound to a highly reactive cysteinyl residue (Cys[superscript]291) of adenylosuccinate synthetase, to active site binding ligands were determined. Energy transfer from formycin 5[superscript]'-monophosphate, a competitive inhibitor of IMP, to Cys[superscript]291, modified with the fluorescent chromophore N-(iodoacetylaminoethyl)-5-naphthylamine-1-sulfonate, indicated that the chromophores are 17 A apart in the presence or absence of GTP. Analogous experiments using tetramethylrhodamine maleimide to modify ...


Molecular Analysis Of Two Adjacent Transcription Terminators In Yeast , Julie A. Peterson Jan 1992

Molecular Analysis Of Two Adjacent Transcription Terminators In Yeast , Julie A. Peterson

Retrospective Theses and Dissertations

RHO1, a member of the ras superfamily of genes, is located on chromosome XIII adjacent to MRP2, a gene coding for mitochondrial ribosomal protein (Madaule and Axel, 1985, Myers, et al. 1987) in Saccharomyces cerevisiae. Nucleotide sequence analysis demonstrated that RHO1 and MRP2 are transcribed convergently and the 3[superscript]' ends of the two coding sequences are separated by only 281 nucleotides (Madaule et al., 1987);The physical relationship between RHO1 and MRP2 was investigated at the transcriptional level by mapping the termini of both transcripts. RHO1 transcripts were found to possess extensive nontranslated regions with jagged initiation and polyadenylation ...


Studies On The Role Of Expression Of Phosphoenolpyruvate Carboxykinase Gene In The Development Of Bovine Lactation Ketosis , Sowmya Moorthi Jan 1992

Studies On The Role Of Expression Of Phosphoenolpyruvate Carboxykinase Gene In The Development Of Bovine Lactation Ketosis , Sowmya Moorthi

Retrospective Theses and Dissertations

Changes in expression of the phosphoenolpyruvate carboxykinase (PEPCK) gene, which codes for a key regulatory enzyme of gluconeogenesis, were studied in bovine liver tissue. Induction of ketosis was attempted in cows by a protocol of restricting feed intake (FR) and supplementing with 1,3-butanediol (BD), a ketogenic precursor, beginning at 15 d postpartum (FRBD protocol). Twenty Holstein cows were distributed in equal numbers among four treatments: normal control, obese control, normal cows given FRBD, and obese cows given FRBD. Liver biopsies were obtained at nine different times during early lactation: 10 d prepartum, 5 and 14 d postpartum (just before ...


Characterization Of A Potato Proteinase Inhibitor Ii Gene Whose Expression In Not Wound-Responsive , Sanggyu Park Jan 1991

Characterization Of A Potato Proteinase Inhibitor Ii Gene Whose Expression In Not Wound-Responsive , Sanggyu Park

Retrospective Theses and Dissertations

A potato Proteinase Inhibitor II gene (pin2T) was isolated and characterized both at the molecular level and the functional level. The open reading frame as well as the 5[superscript]' and 3[superscript]' flanking regions were sequenced. The coding region and 3[superscript]' flanking region showed 86% and 87% identity with those of the previously isolated wound-inducible potato Proteinase Inhibitor IIK gene (pin2K) sequence, respectively. However, the 5[superscript]' flanking region of the pin2T is highly homologous (91% identity) with that of pin2K from -767 to +29, relative to the transcription start site of the wound-inducible pin2K. The 5[superscript ...


Regulation Of Protein Expression By Transforming Growth Factor Beta And Other Growth Modulators , Frederic William Thalacker Jan 1990

Regulation Of Protein Expression By Transforming Growth Factor Beta And Other Growth Modulators , Frederic William Thalacker

Retrospective Theses and Dissertations

The main goal of this project was to understand the signal transduction pathways through which growth modulators such as TGF-[beta], PMA and retinoic acid mediate protein expression and DNA synthesis. Transforming growth factor [beta] (TGF-[beta]) induces synthesis and secretion of a 48,000 M r protein that we have found to be type I plasminogen activator inhibitor (PAI-1). TGF-[beta] induces PAI-1 in every cell line that we have studied including near term mink lung epithelial CCL64 cells, mouse embryo fibroblast AKR-2B 84A cells, African green monkey kidney epithelial BSC-1 cells, and normal rat kidney fibroblast NRK 49F ...


Analysis And Characterization Of The Major Histocompatibility Complex Of Domestic Animals , Yuanxin Xu Jan 1990

Analysis And Characterization Of The Major Histocompatibility Complex Of Domestic Animals , Yuanxin Xu

Retrospective Theses and Dissertations

The major histocompatibility complex (MHC) is a family of genes coding for molecules which play important roles in the immune response as well as many other cellular processes of a vertebrate organism. The understanding of the immune system of some of the domestic animals will have both practical value and theoretical significance. The purpose of this work was to study the MHC of the chicken (B) and the swine (SLA). First, in order to study the MHC class II gene structure of the chicken, a genomic library was constructed from an inbred chicken line G-B2 (MHC haplotype B[superscript]6 ...


The Crystal Structure Of Adenylosuccinate Synthetase From Escherichia Coli , Kyung Hyun Kim Jan 1990

The Crystal Structure Of Adenylosuccinate Synthetase From Escherichia Coli , Kyung Hyun Kim

Retrospective Theses and Dissertations

The crystal structure of adenylosuccinate synthetase from Eschericha coli has been pursued to a resolution of 3.7 A. Using isomorphous and anomalous (SIRAS) phases from a HgI[subscript]4[superscript]2- derivative, electron density maps were generated that clearly revealed the envelope of the enzyme dimer and the two-fold molecular symmetry of the dimer;Since the enzyme is active as a dimer consisting of two identical monomers, the method of density averaging (Bricogne, 1976) was employed to improve the electron density map based on SIRAS phases. The non-crystallographic two-fold operator, which relates two monomers, was refined against an SIRAS ...