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Full-Text Articles in Molecular Biology

Investigation Of The Molecular Mechanism Of Fertility Restoration In Texas Cytoplasm Maize , David Stewart Skibbe Jan 2004

Investigation Of The Molecular Mechanism Of Fertility Restoration In Texas Cytoplasm Maize , David Stewart Skibbe

Retrospective Theses and Dissertations

Cytoplasmic male sterility (cms) is a maternally inherited inability to produce viable pollen. In Texas (T)-cytoplasm maize, the combined action of two restorer of fertility genes, rfl and rfla , can overcome the male sterile phenotype. Previous research established rf2a encodes a mitochondrial aldehyde dehydrogenase (ALDH) with broad substrate specificity. This broad substrate specificity complicates determining the molecular mechanism of rf2a-mediated fertility restoration. In response, a combination of transgenic, biochemical, reverse genetic, and genomic approaches were utilized. Three additional functional maize ALDH genes (rf2b, rf2c, and rf2d) with differing substrate specificities were identified. Each ALDH gene was characterized via in ...


Molecular And Functional Analysis Of Drosophila Hillarin And Its Leech Homolog , Yun Ji Jan 2004

Molecular And Functional Analysis Of Drosophila Hillarin And Its Leech Homolog , Yun Ji

Retrospective Theses and Dissertations

This thesis describes the molecular and functional analysis of the leech Hillarin and its Drosophila homolog D-hil. Leech Hillarin was identified by screening a leech expression library with the mAb Lan3-15, which specifically labels the axon hillock region of leech neurons. Database searches revealed that leech Hillarin has a number of potential homologs in a variety of organisms such as yeast, cyanobacterium, fly, nematode, mouse and human and that these proteins share two novel protein domains, the W180 domain and the H domain. The W180 domain is enriched with tryptophans whereas the H domain shares high number of invariant residues ...


Structural And Molecular Analysis Of Leech Neuronal Proteins, Calsensin And Filamin , Deepa V. Venkitaramani Jan 2004

Structural And Molecular Analysis Of Leech Neuronal Proteins, Calsensin And Filamin , Deepa V. Venkitaramani

Retrospective Theses and Dissertations

This thesis describes the structural and molecular analysis of two leech neuronal proteins, Calsensin and Filamin. Calsensin is an EF-hand calcium-binding protein expressed by a subset of peripheral sensory neurons which fasciculate into a single tract in the leech central nervous system. Calsensin is a 9 kD protein with two EF-hand calcium-binding motifs. Calsensin can multimerize via disulfide bridge formation involving the two cysteine residues in vitro. Furthermore, Calsensin shows differential gel migration depending on the presence or absence of calcium suggesting a conformational change upon calcium binding. Using multidimensional NMR spectroscopy we have determined the solution structure and backbone ...