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Molecular Biology Commons

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Theses/Dissertations

And developmental biology

1992

Articles 1 - 4 of 4

Full-Text Articles in Molecular Biology

Genetic And Biochemical Characterization Of The P21[Superscript Ras]-Related Protein Rho1p In The Yeast Saccharomyces Cerevisiae , Joni Marie Seeling Johnson Jan 1992

Genetic And Biochemical Characterization Of The P21[Superscript Ras]-Related Protein Rho1p In The Yeast Saccharomyces Cerevisiae , Joni Marie Seeling Johnson

Retrospective Theses and Dissertations

Rho1p is a guanine nucleotide binding protein coded for by the Saccharomyces cerevisiae gene RHO1, which is homologous to p21[superscript] ras and other members of the ras superfamily of small molecular weight GTP binding proteins. This protein family is highly conserved in evolution and in some instances is known to be involved in control of cell proliferation. The precise physiological functions of most p21[superscript] ras-related proteins, however, are not known. In order to gain further insight into the physiological function of rho proteins, this research addressed the genetic and biochemical characterization of Rho1p, as well as the subcellular ...


Cloning And Characterization Of Mink Plasminogen Activator Inhibitor Type 1 (Pai-1) Cdna And The Regulation Of Mink Pai-1 Expression At Mrna Level , Tsung-Hsien Chuang Jan 1992

Cloning And Characterization Of Mink Plasminogen Activator Inhibitor Type 1 (Pai-1) Cdna And The Regulation Of Mink Pai-1 Expression At Mrna Level , Tsung-Hsien Chuang

Retrospective Theses and Dissertations

Mink plasminogen activator inhibitor type 1 (PAI-1) is a glycoprotein with molecular weight about 50 kD and is highly regulated in cultured cells. In mink lung CCL64 epithelial cells, epidermal growth factor (EGF), transforming growth factor-[beta] (TGF-[beta]), and 12-O-tetradecanoyl phorbol-13-acetate (TPA) increase the synthesis of PAI-1. Epidermal growth factor and TGF-[beta] synergistically stimulate the synthesis of PAI-1. The synergistic effect and factors which govern the PAI-1 expression in mink lung CCL64 cells were studied by using a mink PAI-1 cDNA as a probe to detect PAI-1 mRNA;The mink PAI-1 cDNA was cloned from a cDNA library ...


Characterization Of The Saccharomyces Cerevisiae Gene Mgp1, A Novel Member Of The Ras Gene Superfamily That Functions In The Mitochondrial Biogenesis , Shu-Hwa Lee Tzeng Jan 1992

Characterization Of The Saccharomyces Cerevisiae Gene Mgp1, A Novel Member Of The Ras Gene Superfamily That Functions In The Mitochondrial Biogenesis , Shu-Hwa Lee Tzeng

Retrospective Theses and Dissertations

This dissertation characterizes Mgp1p, a mitochondrial protein that functions in mitochondrial biogenesis. Mgp1p is the first protein related to the p21[superscript] H-ras oncoprotein shown to function in mitochondria;The Saccharomyces cerevisiae gene MGP1 was isolated based on its ability to restore respiratory competence to a strain containing the nuclear petite mutation msg1-1. MGP1 codes for a protein of approximately 58 kDa, with an amino terminal sequence typical of a mitochondrial targeting peptide. A 190 residue region of Mgp1p shares approximately 30% amino acid sequence identity with any member of p21[superscript] ras-related protein superfamily, with particularly strong homology in ...


Studies On The Role Of Expression Of Phosphoenolpyruvate Carboxykinase Gene In The Development Of Bovine Lactation Ketosis , Sowmya Moorthi Jan 1992

Studies On The Role Of Expression Of Phosphoenolpyruvate Carboxykinase Gene In The Development Of Bovine Lactation Ketosis , Sowmya Moorthi

Retrospective Theses and Dissertations

Changes in expression of the phosphoenolpyruvate carboxykinase (PEPCK) gene, which codes for a key regulatory enzyme of gluconeogenesis, were studied in bovine liver tissue. Induction of ketosis was attempted in cows by a protocol of restricting feed intake (FR) and supplementing with 1,3-butanediol (BD), a ketogenic precursor, beginning at 15 d postpartum (FRBD protocol). Twenty Holstein cows were distributed in equal numbers among four treatments: normal control, obese control, normal cows given FRBD, and obese cows given FRBD. Liver biopsies were obtained at nine different times during early lactation: 10 d prepartum, 5 and 14 d postpartum (just before ...