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Full-Text Articles in Molecular Biology
Production And Use Of Monoclonal Antibodies For Molecular Characterization, Affinity Purification, And Serological Detection Of Economically Significant Plant Viruses , Robert Diaco
Retrospective Theses and Dissertations
Hybridomas secreting monoclonal antibodies (M-Abs) against three isolates of barley yellow dwarf virus (BYDV) were established. Two M-Abs were generated against the MAV isolate, one against RPV, and six against P-PAV. None of the M-Abs reacted with healthy host components. Reactions of M-Abs, or unlabelled polyclonal antisera, in an indirect enzyme-linked immunosorbent assay (ELISA) indicate the presence of a common epitope on all three virus isolates. BYDV dissociated when incubated in carbonate-bicarbonate coating buffer at pH 9.6; stabilization was achieved by predialysis with either 2% formaldehyde or 2% glutaraldehyde. In indirect ELISA, unlabelled polyclonal antisera bound to both stabilized ...