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Studying The Replication Mechanism Of The Yeast Retrotransposon Ty5 By Molecular And Computational Approaches, Xiang Gao
Retrospective Theses and Dissertations
The yeast retrotransposon Ty5 is a Ty1/copia element. It is in the Hemivirus genus of the Pseudoviridae family. The ability to genetically manipulate retrotransposons and the yeast host cell was taken advantage of to explore replication mechanisms unique to Ty5 and common to most retrotransposons. Because of the abundance and diversity of retroelement sequences, along with the fact that many retroelement enzymes have evolved unique functional specificities, computational approaches were also developed to study functional divergence in replication. By screening a randomly mutagenized Ty5 library, two mutations (Y68C, D252N) causing higher transposition frequencies were identified. Both mutations increased Ty5 ...