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Molecular Biology Commons

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Theses/Dissertations

And developmental biology

Genetics

1997

Articles 1 - 2 of 2

Full-Text Articles in Molecular Biology

Genetic And Biochemical Analysis Of Aspergillus Awamori Glucoamylase Thermostability , Martin John Allen Jan 1997

Genetic And Biochemical Analysis Of Aspergillus Awamori Glucoamylase Thermostability , Martin John Allen

Retrospective Theses and Dissertations

Aspergillus awamori glucoamylase (GA) is an enzyme involved in industrial corn starch processing. Genetic and biochemical approaches were used to study the mechanisms governing GA thermostability. Three proline substitution (Xaa → Pro) mutations were constructed that were predicted to increase the enzyme's stability by decreasing its conformational entropy of unfolding. When expressed in Saccharomyces cerevisiae Ser30 → Pro increased, Asp345 → Pro did not alter and Glu408 → Pro greatly decreased GA stability as measured by resistance to irreversible thermoinactivation relative to the wild-type enzyme. The Ser30 → Pro mutation was combined with other previously identified stabilizing mutations to examine whether combining such mutations ...


Suppressor Analysis Of The Protein Kinase Elm1p, An Enzyme Involved In The Regulation Of Yeast Morphology , Nicholas Patrick Edgington Jan 1997

Suppressor Analysis Of The Protein Kinase Elm1p, An Enzyme Involved In The Regulation Of Yeast Morphology , Nicholas Patrick Edgington

Retrospective Theses and Dissertations

Saccharomyces cerevisiae is an ascomycete that has several developmental options, with concomitant morphological changes, that depend on cell type and nutrient status. In response to these signals, vegetative Saccharomyces cerevisiae cells assume one of two distinct morphologies, the yeast form or the filamentous form. Molecules affecting this morphogenetic response were identified by analyzing transposon-induced or spontaneous suppressor mutations that restore yeast form growth to a strain that constitutively exhibits filamentous form characteristics owing to mutation of the protein kinase Elm1p. One transposon-tagged mutation was isolated and is allelic to the negative regulatory subunit Bcy1p, thus activation of cAMP dependent protein ...