Molecular Biology Commons^™

Functions Of Mitoneet: What The Protein-Protein Interactions Reveal, Fran Hirschfelder

Masters Theses

MitoNEET is a [2Fe-2S] cluster protein found on the outer mitochondrial membrane. First discovered as a binding site for pioglitazone, an anti-diabetic drug, it was the first protein described containing a CDGSH iron-sulfur domain (CISD). However, the function(s) of mitoNEET in both normal cellular physiology and the physiology in type-2 diabetes remains unclear. The proposed functions of mitoNEET, include cluster transfer, electron transport and oxidative respiration. This thesis describes the use of proteomic pull-down techniques to identify possible protein binding partners of mitoNEET from both mouse and human liver cell lysates. In order to accomplish this, the expression and purification …