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Theses/Dissertations

2000

Articles 1 - 17 of 17

Full-Text Articles in Molecular Biology

Molecular Evolution Of Insecticidal Spore-Forming Bacteria, John Pool Apr 2000

Molecular Evolution Of Insecticidal Spore-Forming Bacteria, John Pool

Honors Theses

Molecular methods are increasingly being used to determine the phylogeny of microorganisms. This research was intended to determine phylogenetic relationships for bacteria of the species Bacillus thuringiensis and other members of the Bacillus cereus group. Each strain was analyzed by its sasp-B gene sequence to determine its species classification and relation to other strains studied. Results of this study indicated that according to the sasp-B gene tree, the species Bacillus thuringiensis is a paraphyletic with respect to both Bacillus cereus and Bacillus anthracis. Some unexpected results and implications for species designations are also discussed.


Characterization Of Human T-Cell Leukemia Virus 1 Reverse Transcriptase, Pinky Gundayao Agbuya Apr 2000

Characterization Of Human T-Cell Leukemia Virus 1 Reverse Transcriptase, Pinky Gundayao Agbuya

Theses and Dissertations in Biomedical Sciences

Human T-cell leukemia virus 1 (HTLV-1) is a type C human retrovirus which has been the causative agent of Adult T-cell leukemia. Replication of the retrovirus; requires a reverse transcriptase which converts the retroviral RNA into DNA which is later incorporated into the host's genome. Very little is known about the reverse transcriptase of HTLV-1. Researchers have attempted to purify HTLV-1 RT by isolating the enzyme from human cell lines. Because large amounts of protein could not be produced by this isolation method, the reverse transcriptase cannot be fully characterized. In this research, a recombinant protein expressed in E ...


Carbohydrate Regulation Of Leaf Development: Investigating The Role Of Source Strength, Carbon Partitioning And Hexokinase Signaling In Regulating Leaf Senescence , Adam Christopher Miller Jan 2000

Carbohydrate Regulation Of Leaf Development: Investigating The Role Of Source Strength, Carbon Partitioning And Hexokinase Signaling In Regulating Leaf Senescence , Adam Christopher Miller

Retrospective Theses and Dissertations

For many plants, growth in elevated CO2 leads to reduced rates of photosynthesis. To examine the role leaf ontogeny plays in acclimation; we monitored photosynthesis and some related parameters throughout the development of tobacco leaves under ambient and high CO2 conditions. The pattern of photosynthetic rate over time was consistent with the expected pattern for a typical dicot leaf for both. However, the photosynthesis pattern in high-CO2-grown tobacco was shifted temporally to an earlier maximum and subsequent senescent decline. Rubisco activity appeared to be the main factor regulating photosynthetic rates in both treatments. Therefore, we propose a new model for ...


Mechanisms Of Negative Regulation Of Protein Prenylation: Investigation Of Murine Guanylate-Binding Protein 1 , John Thomas Stickney Jan 2000

Mechanisms Of Negative Regulation Of Protein Prenylation: Investigation Of Murine Guanylate-Binding Protein 1 , John Thomas Stickney

Retrospective Theses and Dissertations

Covalent attachment of isoprenoid lipids to proteins is a post-translational modification that occurs on approximately 2% of all cellular proteins. Proteins that contain a CaaX motif as their final four amino acids are presumed to be targets for the addition of C15 or C20 isoprenoids by farnesyl transferase (FTase) or geranylgeranyl transferase I (GGTase I) respectively. This modification is generally believed to occur stoichiometrically, and irreversibly, in vivo. Thus, if prenylation is to be regulated, this control must occur prior to lipid addition. At the moment, there is little evidence that the enzymatic activity of prenyl transferases can be modulated ...


Genetic Variation And Evolution Of Equine Infectious Anemia Virus Rev Quasispecies During Long Term Persistent Infection , Prasith Baccam Jan 2000

Genetic Variation And Evolution Of Equine Infectious Anemia Virus Rev Quasispecies During Long Term Persistent Infection , Prasith Baccam

Retrospective Theses and Dissertations

Genetic variation has been observed in many viruses. Viruses that carry their genetic information in the form of RNA exhibit high mutation rates because the viral polymerase lacks proof-reading mechanisms commonly found in DNA polymerase complexes. The combination of high mutation rates, small genome size, and high replication rates results in a population of closely related viral genotypes, which are commonly referred to as a quasispecies. A consequence of the genetic variation in viruses is possible variation in viral phenotype of the quasispecies population. Furthermore, changes in viral phenotype may be a biologically important factor in progression of disease. Here ...


Function And Regulation Of Alternative Isoforms Of The Transcription Factor Pax-5 During B Lymphocyte Differentiation, Marina A. Lowen Jan 2000

Function And Regulation Of Alternative Isoforms Of The Transcription Factor Pax-5 During B Lymphocyte Differentiation, Marina A. Lowen

Dissertations, Theses, and Masters Projects

No abstract provided.


The Specific Immune Response In Rainbow Trout: Somatic Hypermutation And Vh Gene Utilization, Teresa D. Lewis Jan 2000

The Specific Immune Response In Rainbow Trout: Somatic Hypermutation And Vh Gene Utilization, Teresa D. Lewis

Dissertations, Theses, and Masters Projects

The study of antibody responses in prominent aquaculture species such as the rainbow trout, Oncorhynchus mykiss, can facilitate vaccine development and contribute to producing useful paradigms of adaptive immunity in lower vertebrates. Thus, it is essential to identify genes responsible for antibody responses. In the mouse model, hybridoma technology allows for the association of monoclonal antibodies possessing various affinities for antigen with specific VH sequences, gene family utilization, and other molecular events (i.e. somatic hypermutation) that occur during the specific immune response. The absence of a comparable hybridoma technology in piscine systems has limited similar studies of fish immunogenetics ...


Two Dimensional Models Of Tumor Angiogenesis , Serdal Pamuk Jan 2000

Two Dimensional Models Of Tumor Angiogenesis , Serdal Pamuk

Retrospective Theses and Dissertations

Angiogenesis, the formation of new capillaries from pre-existing vessels, is essential for tumor progression. It is critical for the growth of primary cancers. In this thesis we present a new approach to angiogenesis, based on the theory of reinforced random walks, coupled with a Michaelis-Menten type mechanism. This views the endothelial cell receptors as the catalyst for transforming angiogenic factor into proteolytic enzyme in order to model the first stage. In our model we use a curvature-induced proliferation term for the endothelial cell equation. Our numerical results indicate that the proliferation of endothelial cells is high at the tip. Also ...


Characterization Of [Alpha]-Adrenoceptor-Mediated Contractile Responses In The Isolated Bovine Tail Artery And Vein And An Investigation Of Nerve-Mediated Contraction In The Bovine Tail Artery In Response To Electrical Field Stimulation , Marina Val Ioudina Jan 2000

Characterization Of [Alpha]-Adrenoceptor-Mediated Contractile Responses In The Isolated Bovine Tail Artery And Vein And An Investigation Of Nerve-Mediated Contraction In The Bovine Tail Artery In Response To Electrical Field Stimulation , Marina Val Ioudina

Retrospective Theses and Dissertations

The purpose of this study was to identify alpha-adrenoreceptor types and subtypes mediating contractile responses to adrenergic agonists in the bovine tail artery and vein and to investigate nerve-mediated contraction in the bovine tail artery in response to electrical field stimulation. Isolated tissue bath methods were used to study isometric contractile responses in vessel rings. Electrical field stimulation, under constant current conditions, was used to study vessel contraction as a result of neurotransmitter(s) release from sympathetic nerve terminals;Evidence was obtained for the presence of functional alpha1A- and alpha1D-adrenoceptors in the tail artery. Whether functional alpha 1B-adrenoceptors are present ...


Tissue Specific Expression And Regulation Of The Mitogen Regulated Protein Genes , John Tollen Fassett Jan 2000

Tissue Specific Expression And Regulation Of The Mitogen Regulated Protein Genes , John Tollen Fassett

Retrospective Theses and Dissertations

Mitogen Regulated Protein (MRP; also called Proliferin; or MRP/PLF) is a member of the Prolactin/Growth Hormone gene family. There is evidence that MRP/PLF is a uterine growth factor and an angiogenesis factor. Between four and six highly homologous MRP/PLF genes (mrp/plf ) exist in the mouse, but the significance of multiple genes has not been determined. MRP/Plf is produced at high levels during mid-gestation by the placenta, where expression is restricted to the trophoblastic giant cells. To better understand the regulation of the mrp/ plf genes in vivo, we have developed an assay to analyze ...


Bacteriophage Therapy: A Novel Method Of Lytic Phage Delivery , Ratree Platt Jan 2000

Bacteriophage Therapy: A Novel Method Of Lytic Phage Delivery , Ratree Platt

Retrospective Theses and Dissertations

The widespread emergence of multi-antibiotic resistant bacteria has increased the need for alternatives to conventional antibiotic therapy. Accordingly, a significant amount of effort has been made to investigate the potential use of bacteriophages as prophylactic and therapeutic agents for bacterial infections. In this study, molecular biological techniques were applied to construct a lysogen of lytic bacteriophage lambda in an attempt to combat with multi-antibiotic resistant bacteria by a novel method of lytic phage delivery;To accomplish this goal, two plasmid-based site-specific recombination (SSR) systems for integration and recovery of DNA constructs from Escherichia coli and Salmonella typhimurium chromosomes were developed ...


Biochemical And Molecular Genetic Studies Of The Metabolic Role Of Methylcrotonyl-Coa Carboxylase , Ping Che Jan 2000

Biochemical And Molecular Genetic Studies Of The Metabolic Role Of Methylcrotonyl-Coa Carboxylase , Ping Che

Retrospective Theses and Dissertations

The physiological metabolic roles of methylcrotonyl-CoA carboxylase (MCCase) have been investigated. Radiotracer metabolic studies using extracts from isolated mitochondria and incubated with [U-14C]leucine and NaH14CO3, demonstrate that plant mitochondria can catabolize leucine via the following scheme: leucine ⇒ alpha-ketoisocaproate ⇒ isovaleryl-CoA ⇒ 3-methylcrotonyl-CoA ⇒ 3-methylglutaconyl-CoA ⇒ 3-hydroxy-3-methylglutaryl-CoA ⇒ acetoacetate + acetyl-CoA. These findings demonstrate for the first time that the enzymes responsible for leucine catabolism are present in plant mitochondria. We conclude that one of the primary metabolic roles of MCCase in plants is the catabolism of leucine;A single-copy gene encoding the Arabidopsis MCC-A subunit was isolated and characterized. It contains 15 exons separated by ...


Structure And Function Of A Novel Cap-Independent Translation Element In The 3' Untranslated Region Of A Viral Rna , Liang Guo Jan 2000

Structure And Function Of A Novel Cap-Independent Translation Element In The 3' Untranslated Region Of A Viral Rna , Liang Guo

Retrospective Theses and Dissertations

This research focuses on a cap-independent translation element (TE) we identified in the RNA of barley yellow dwarf virus (BYDV). Assisted by the TE, all messenger RNAs of BYDV, which include BYDV genomic RNA and subgenomic RNAs, are translated cap-independently. This allows uncapped, nonpolyadenylated BYDV RNAs to compete with capped and polyadenylated host mRNAs for the translation machinery. The core of the TE is a 105 nt sequence (TE105) located in the 3' UTR of BYDV genomic RNA. Due to its 3' position, at least two functions are necessary for TE105: 3 '--5' communication, and recruitment of translation machinery. TE105 ...


Characterization Of Intracellular Survival Of Mycobacterium Avium Subspecies Paratuberculosis In J774 Murine Macrophages , Jesse Michael Hostetter Jan 2000

Characterization Of Intracellular Survival Of Mycobacterium Avium Subspecies Paratuberculosis In J774 Murine Macrophages , Jesse Michael Hostetter

Retrospective Theses and Dissertations

Mycobacterium avium subspecies paratuberculosis (M. a. ptb) is the causative agent of Johne's disease, a progressive diarrheal disease of wild and domestic ruminants. Like other pathogenic species of mycobacteria, M. a. ptb is an intracellular pathogen of monocytes and monocyte-derived macrophages. In this dissertation we tested the hypothesis that M. a. ptb survives within macrophages by inhibition of acidification and maturation of the phagosomal compartment in which it resides;Chapter 1 consists of a general introduction and a literature review of recent reports concerning normal phagosomal maturation and maturation of the mycobacterial phagosome. Chapter 2 characterizes acidification of the ...


Expression Of Bovine Mammary Gland Ca2+Atpases , Siriwan Prapong Jan 2000

Expression Of Bovine Mammary Gland Ca2+Atpases , Siriwan Prapong

Retrospective Theses and Dissertations

Control of transcellular Ca2+ fluxes associated with lactation are critical to mammary function and Ca2+ homeostasis. Mammary Ca2+ transport and storage may contribute to the pathological hypocalcemia seen in dairy cows at parturition. RT-PCR and Western Blotting were used to identify which Ca2+ ATPases are present in the bovine mammary gland and in milk fat globule membranes (MFGM). PMCA1, PMCA2 and PMCA4 are plasma membrane Ca2+ ATPases expressed in the bovine mammary gland. A putative Golgi Ca2+ ATPase (bSPCA) is also found in significant amounts in the mammary gland. The expression of Ca2+ ATPases in the bovine mammary gland is ...


Characterization Of Skeletor: A Novel Nuclear Protein Implicated In Mitotic Spindle Assembly , Diana Lee Walker Jan 2000

Characterization Of Skeletor: A Novel Nuclear Protein Implicated In Mitotic Spindle Assembly , Diana Lee Walker

Retrospective Theses and Dissertations

The mAb 2A labels Drosophila embryos in a novel, dynamic staining pattern that identifies a nuclear meshwork during interphase, which is remodeled to form a spindle structure at metaphase. This intriguing pattern made the antigens recognized by mAb 2A interesting targets for cloning and analysis, Skeletor was found to be encoded by a complex locus. Two alternatively spliced transcripts from the Skeletor locus were identified, Orf1b/Orf2 and Orf1a. Orf1b/Orf2 has an unusual structure, containing two overlapping open reading frames with coding potentials of 87 kD and 81 kD respectively. Analysis of Orf2 amino acid content reveals overall high ...


Human Gonadotropin-Releasing Hormone (Hgnrh) Gene Expression And Hormone Regulation In Human Placental Jeg-3 Cells, Heming Zheng Jan 2000

Human Gonadotropin-Releasing Hormone (Hgnrh) Gene Expression And Hormone Regulation In Human Placental Jeg-3 Cells, Heming Zheng

Theses and Dissertations in Biomedical Sciences

Using the human placental choriocarcinoma JEG-3 cell line as an in vitro human placental model, I studied the mechanisms of the tissue-specific expression and steroid hormone regulation of the hGnRH gene in the human placenta. The results showed that all of the previously identified four elements are required for the full activity of the hGnRH upstream promoter in JEG-3 cells, while the element 4 (FP4, −987/−968) is the most important. Studies performed with 5′ end deletion of this region confirmed these observations. Further, supershift assay using Oct-1 antibody demonstrated the involvement of Oct-1 in the FP4 DNA-protein interaction in ...