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Theses/Dissertations

1998

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Articles 1 - 24 of 24

Full-Text Articles in Molecular Biology

In Vitro Assessment Of The Toxicity Of Cocaine And Its Metabolites In The Human Umbilical Artery, Tessa L. Long Aug 1998

In Vitro Assessment Of The Toxicity Of Cocaine And Its Metabolites In The Human Umbilical Artery, Tessa L. Long

Electronic Theses and Dissertations

An in vitro model was used to assess the effect of cocaine and its metabolites on the umbilical artery. Objectives were to pharmacologically confirm the presence of adrenergic innervation using tyramine, evaluate the ability of cocaine, benzoylecgonine, norcocaine and cocaethylene to potentiate vasoconstriction by serotonin and norepinephrine, examine the ability of ketanserin to block the enhanced vasoconstriction produced by cocaine, and determine displacement of 3 H-ketanserin by serotonin, norepinephrine, tyramine and mianserin. The vasoconstrictive effect of tyramine (100 μM) was enhanced in the presence of cocaine by 257%. Vasoconstrictive effects of serotonin and norepinephrine were significantly enhanced by cocaine by ...


Characterization Of The Biological Functions Of Human Recombinant Zona Pellucida Protein 3, Yu Wen Juan Jul 1998

Characterization Of The Biological Functions Of Human Recombinant Zona Pellucida Protein 3, Yu Wen Juan

Theses and Dissertations in Biomedical Sciences

Recombinant human zona pellucida protein 3 (rhZP3), expressed. isolated and purified from PA-1 cells, is characterized for its biological activity and the role in the signal transduction pathway. Characterization of the biological activity of rhZP3 was detected by hemizona assay and immunofluorescence staining of acrosome reaction The results indicated that rhZP3 exhibited an inhibition in the binding assay (HZI 43.6 +/-3.3; n = 9; 30 ng/mL rhZP3) and induction of acrosome reaction (198.6% +/- 77.2% increase from baseline; n = 29; 30 ng/mL rhZP3). It was further confirmed by the transmission electron microscopy that there was no ...


Expression, Isolation And Purification Of Human Zona Pellucida Protein 3, Ting-Fung Chi Jul 1998

Expression, Isolation And Purification Of Human Zona Pellucida Protein 3, Ting-Fung Chi

Theses and Dissertations in Biomedical Sciences

Mammalian fertilization involves interactions of sperm surface receptors with the ligands of the zona pellucida, an extracellular matrix surrounding the ovulated oocytes. In humans, the zona pellucida is composed of three major glycoproteins. One of them, ZP3, participates in the primary sperm binding and in the subsequent triggering of the spermatozoa's acrosome reaction. Studies on the role of this specific protein in the human fertilization process are hampered by the limited amount of available biologically functional proteins.

By use of a pcDNA 3.1(+) expression vector, a transfecting-vector was constructed containing a 1.3 kb histidine tagged hZP3 cDNA ...


Leukemia Inhibitory Factor (Lif): Murine Preimplantation Embryo Development, Implantation Rates, And Skeletal Development, Michael Hayes Mitchell Jul 1998

Leukemia Inhibitory Factor (Lif): Murine Preimplantation Embryo Development, Implantation Rates, And Skeletal Development, Michael Hayes Mitchell

Theses and Dissertations in Biomedical Sciences

Leukemia inhibitory factor (LIF) is a pleiotropic cytokine which demonstrates perplexing physiological effects. It has been demonstrated that LIF is essential for implantation in mice. Little is known relating to the manner by which LIF effects pre-implantation and post-implantation development. The objectives of this project were to determine the effects LIF on pre-implantation development, to determine the effects that it may have on implantation rates, successful pregnancy rates, and resorption rates, and to determine the effects that LIF has on the skeletal development of mice. For the embryo transfer experiments, embryos were exposed to test compounds in the transfer medium ...


The Influence Of A Human Repetitive Dna On Genome Stability, Eugenia L. Posey May 1998

The Influence Of A Human Repetitive Dna On Genome Stability, Eugenia L. Posey

Electronic Theses and Dissertations

A uniquely human interspersed repetitive DNA sequence family, the L2Hs, are highly polymorphic in human genomes. Several features of interspersed repeated DNA may contribute to the instability observed. Certain motifs (direct repeats, palindromes, and inverted repeats) comprising L2Hs elements may adopt unusual secondary structures such as cruciforms or hairpins. These motifs have been associated with features of genome instability in recombination, insertions and deletions. The L2Hs elements also are AT-rich (76%) compared to the bulk of human DNA (52%). That their dynamic nature (i.e. polymorphisms) may arise from recombination, insertions and deletions has led to the hypothesis that the ...


Transcriptional Regulation Of A Human H4 Histone Gene Is Mediated By Multiple Elements Interacting With Similar Transcription Factors: A Dissertation, Thomas J. Last May 1998

Transcriptional Regulation Of A Human H4 Histone Gene Is Mediated By Multiple Elements Interacting With Similar Transcription Factors: A Dissertation, Thomas J. Last

GSBS Dissertations and Theses

Synthesis of histone proteins occurs largely during the S phase of the cell cycle and coincides with DNA replication to provide adequate amounts of histones necessary to properly package newly replicated DNA. Controlling transcription from cell cycle dependent and proliferation specific genes, including histone H4, is an important level of regulation in the overall governance of the cell growth process. Coordination of histone gene transcription results from the cumulative effects of cell signaling pathways, dynamic chromatin structure and multiple transcription factor interactions. The research of this dissertation focused on the characterization and identification of transcription factors interacting on the human ...


The Phylogeny Of Coleoid Cephalopods Inferred From Molecular Evolutionary Analyses Of The Cytochrome C Oxidase I, Muscle Actin, And Cytoplasmic Actin Genes, David Bruno Carlini Jan 1998

The Phylogeny Of Coleoid Cephalopods Inferred From Molecular Evolutionary Analyses Of The Cytochrome C Oxidase I, Muscle Actin, And Cytoplasmic Actin Genes, David Bruno Carlini

Dissertations, Theses, and Masters Projects

Although the fossil record of early cephalopods is rich and demonstrates the dominance of the group in Paleozoic times, the mainly soft-bodied coleoids (Cephalopoda: Coleoidea) are poorly represented. Therefore, little is known of the evolutionary history of coleoids through paleontology and current classifications of the subclass are based primarily on the morphology of extant representatives. A molecular phylogenetic analysis of the Coleoidea was therefore warranted. Phylogenetic relationships within the Coleoidea were constructed using molecular sequence data from one mitochondrial and two nuclear genes: cytochrome c oxidase I (COI) and two unlinked actin genes (Actin I and Actin II, respectively). A ...


Genetic Analysis Of Quantitative Trait Loci With The Early Generations Of An Elite, Single-Cross Maize Population , Dwi Asmono Jan 1998

Genetic Analysis Of Quantitative Trait Loci With The Early Generations Of An Elite, Single-Cross Maize Population , Dwi Asmono

Retrospective Theses and Dissertations

The first objective of this study was to detect QTLs affecting agronomically important traits in the F2 plants of an elite, single-cross of maize (Zea mays L.). The second objective was to compare QTL detection in the F2 and F2:3 generations. The third objective was to compare QTL detection in two samples of F2:3 lines derived from the same F2 population;Thirty-eight QTLs affecting five traits were identified in the F2 plants. The QTLs were associated with 6 to 43% of the phenotypic variation. QTLs explaining more than 40% of the variation were observed for plant height and ...


Reversible Palmitoylation Of H-Ras Is Affected By Oxidants And Activation State , Tara Lynn Baker Jan 1998

Reversible Palmitoylation Of H-Ras Is Affected By Oxidants And Activation State , Tara Lynn Baker

Retrospective Theses and Dissertations

Signal transduction organizes cell growth, differentiation, and apoptosis. One component of several signaling pathways leading to these outcomes is the Ras protein. Ras is a small GTPase, binding GTP in an active conformation and GDP in an inactive conformation. Upon nucleotide binding, the conformation of Ras changes so that its effector region can bind to proteins directly downstream in several pathways. Several GTP-bound mutants have been discovered, and an activated Ras mutant has been found in at least 50% of human tumors;Ras must reside at the membrane in order to interact with effector proteins. Binding at the plasma membrane ...


Epigenetic Effects Of Matrix Attachment Regions On Transgene Expression In A Maize Callus Line , Cory Ray Brouwer Jan 1998

Epigenetic Effects Of Matrix Attachment Regions On Transgene Expression In A Maize Callus Line , Cory Ray Brouwer

Retrospective Theses and Dissertations

Matrix attachment regions (MARs) are DNA sequences that bind non-histone proteins in the chromatin scaffold and define discrete loops of DNA containing expressed genes in vivo. I have been studying the effects of flanking transgenes with MARs on transgene expression levels in BMS maize callus. Three MAR elements, two from maize (Adhl 5' MAR and Mhal 5' MAR) and one from yeast (ARS1), have very different effects on transgene expression that bear no relation to their affinity for the nuclear matrix in vitro. The main effect of MARs is to prevent silencing of some (but not all) transgenes, at least ...


Theoretical Simulations And Ultrafast Pump-Probe Spectroscopy Experiments In Pigment-Protein Photosynthetic Complexes , Daniel Richard Buck Jan 1998

Theoretical Simulations And Ultrafast Pump-Probe Spectroscopy Experiments In Pigment-Protein Photosynthetic Complexes , Daniel Richard Buck

Retrospective Theses and Dissertations

Theoretical simulations and ultrafast pump-probe laser spectroscopy experiments were used to study photosynthetic pigment-protein complexes and antennae found in green sulfur bacteria such as Prosthecochloris aestuarii, Chloroflexus aurantiacus, and Chlorobium tepidum. The work focused on understanding structure-function relationships in energy transfer processes in these complexes through experiments and trying to model that data as we tested our theoretical assumptions with calculations;Theoretical exciton calculations on tubular pigment aggregates yield electronic absorption spectra that are superimpositions of linear J-aggregate spectra. The electronic spectroscopy of BChl c/d/e antennae in light harvesting chlorosomes from Chloroflexus aurantiacus differs considerably from J-aggregate spectra ...


Isolation And Characterization Of Genes Belonging To Developmental Regulatory Gene Classes In Potato (Solanum Tuberosum L.) , Jennifer Krulic Hart Jan 1998

Isolation And Characterization Of Genes Belonging To Developmental Regulatory Gene Classes In Potato (Solanum Tuberosum L.) , Jennifer Krulic Hart

Retrospective Theses and Dissertations

Although plant growth is an important topic, the regulation of genes involved in growth is poorly understood. To contribute to current information about plant growth regulation, an early tuberization cDNA library from Solanum tuberosum L., expected to contain genes involved in cell proliferation, was screened for members of developmental regulatory gene families. The two objectives of this study were to isolate genes that might be involved in plant growth regulation, and to characterize the RNA accumulation patterns of these genes to gain insight into their function. The AAA-protein superfamily ATPase POTATP1 and the homeobox gene POTH1 were isolated to accomplish ...


Potato Lipoxygenase Genes: Their Role In Tuber Formation And Defense Responses Against Pathogens , Mikhailo V. Kolomiets Jan 1998

Potato Lipoxygenase Genes: Their Role In Tuber Formation And Defense Responses Against Pathogens , Mikhailo V. Kolomiets

Retrospective Theses and Dissertations

Lipoxygenase (LOX) is involved in the synthesis of jasmonic acid, methyl jasmonate, traumatin, and C-6 volatile compounds that serve hormone-like regulatory and defense-related roles in plants. To study possible lipoxygenase involvement in potato tuberization and defense responses against pests, we have isolated, sequenced, and characterized three lipoxygenase cDNA clones that represent distinct LOX genes. Two of these genes, designated POTLX-1 and POTLX-2, have been isolated from an early tuber cDNA library. Northern hybridization studies indicated that accumulation of POTLX-1 and POTLX-2 mRNA was specific to developing tubers and roots and correlated positively with tuber growth. Hormonal treatment, pathogen infection, or ...


Analysis Of Viral Proteins Of Porcine Reproductive And Respiratory Syndrome Virus In Host Protection And Early Virus-Cell Interactions , Yanjin Zhang Jan 1998

Analysis Of Viral Proteins Of Porcine Reproductive And Respiratory Syndrome Virus In Host Protection And Early Virus-Cell Interactions , Yanjin Zhang

Retrospective Theses and Dissertations

The viral proteins of porcine reproductive and respiratory syndrome virus (PRRSV) were expressed in a baculovirus expression system. They were evaluated for protection of pigs against the respiratory disease and for function in virus-cell interactions. The open reading frames 2 to 6 products were confirmed to be membrane associated proteins as they were detected on the surface of viable insect cells by an immunofluorescence assay. Immunization of pigs with vaccines comprised of a combination of GP2, GP3 and GP4 or a combination of GP5, M and N proteins induced partial protection, but immunization with combination of GP5 and M proteins ...


Characterization Of Starch Debranching Enzymes Of Maize Endosperm , Afroza Rahman Jan 1998

Characterization Of Starch Debranching Enzymes Of Maize Endosperm , Afroza Rahman

Retrospective Theses and Dissertations

The goal of this research is to understand the biochemical lesion of the sugary1 mutation in maize. The sugary1 gene is a determinant of starch structure. Homozygous su1-mutant endosperms accumulate a highly-branched water-soluble polysaccharide, phytoglycogen, at the expense of amylopectin. These suggest that both branched polysaccharides share a common biosynthetic pathway, and that SU1 participates in the biosynthesis of kernel starch. Specific antibodies were used to demonstrate that SU1 is a 79-kD protein that accumulates in endosperm during starch biosynthesis. Recombinant near full-length SU1 (SU1r) was expressed in E. coli and purified to apparent homogeneity. Determination of substrate specificity of ...


Comparative Evolution Of Molecular Markers: An Analysis Of Genetic Variation Within The Blue Marlin (Makaira Nigricans), Vincent Patrick Buonaccorsi Jan 1998

Comparative Evolution Of Molecular Markers: An Analysis Of Genetic Variation Within The Blue Marlin (Makaira Nigricans), Vincent Patrick Buonaccorsi

Dissertations, Theses, and Masters Projects

Blue marlin diversity was assessed at mtDNA, scnDNA, microsatellite DNA, and allozyme molecular markers. Hierarchical analysis of molecular variance (AMOVA) revealed that most genetic variation was maintained within populations, with a non-significant fraction attributable to variation among temporal replicates and between locations within oceans. In contrast, inter-ocean divergence was highly significant for a majority of loci within each marker class. Previous studies of mitochondrial DNA (mtDNA; n = 104) genetic variation within the blue marlin revealed two distinct clades of haplotypes, one of which was present only in the Atlantic (the 'Atlantic clade'), at a frequency of 40% &(F\sb{lcub ...


Characterization Of Three Root Hair Mutants In Maize , Tsui-Jung Wen Jan 1998

Characterization Of Three Root Hair Mutants In Maize , Tsui-Jung Wen

Retrospective Theses and Dissertations

Root hairs are thought to play an important role in the uptake of water and nutrients from the environment. Several root hair defective mutants were isolated from Mutator-tagged stocks and EMS-mutagenized lines of maize. These mutants all condition either non-elongated root-hair initials (rth1, rth3-1, rth3-2, rth3-3, rth4, rth5, rth6) or shorter-than-normal root hairs (rth2). The morphology of rth1, rth2, and rth3 root hairs were analyzed via SEM. The rth1, rth2, and rth3 genes map to chromosomes 1L, 5L, and 1S, respectively. The rth3 gene was cloned using Mu-tagged allele. The partial cDNA sequence of this gene suggests that it may ...


Partial Sequencing Of A Balbiani Ring Gene And Development Of A Molecular Probe, Kerri Ann Kurzeja Jan 1998

Partial Sequencing Of A Balbiani Ring Gene And Development Of A Molecular Probe, Kerri Ann Kurzeja

Seton Hall University Dissertations and Theses (ETDs)

The Environmental Protection Agency is currently developing sediment quality criteria in order to protect the nation's water sources. In order to assist in the development of standards for the disposal of heavy metals, our laboratory is undergoing sediment toxicity studies on Chironomus riparius. It is an EPA approved test organism that spends most of its life in the sediment. Following a previous study (Cochrane et al., 1994), the organism's response to heavy metals will be monitored on the genetic level. Several genes, including a Balbiani ring (BR) gene, which was the focus of this thesis, will be studied ...


Identification Of An Amylase-Like Gene In Chironomus Riparius, Loriann Mahurter Jan 1998

Identification Of An Amylase-Like Gene In Chironomus Riparius, Loriann Mahurter

Seton Hall University Dissertations and Theses (ETDs)

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The Poplar-Leaf Rust Pathosystem: Inheritance Of Resistance And Pathogenic Variability , Girma Melesse Tabor Jan 1998

The Poplar-Leaf Rust Pathosystem: Inheritance Of Resistance And Pathogenic Variability , Girma Melesse Tabor

Retrospective Theses and Dissertations

Among diseases that cause serious yield reduction on Populus deltoides is leaf rust caused by Melampsora medusae. The objectives of this project were to study the pathogenic variability of M. medusae, the genetic basis of inheritance of M. medusae resistance in a family of P. deltoides; and to identify molecular markers linked to a leaf-rust resistant locus. Three pathotypes of M. medusae were identified on the basis of their differential reaction to three Populus clones. Pathotypes D-93, F-93, and IL-48 were compatible with clone 1-488 (P. X euramericana). F-93 and IL48 were also compatible with clone 57-276 (P. deltoides X ...


The Role Of Pit1 In The Putative Quantitative Trait Loci Region On Pig Chromosome 13 , Tun-Ping Yu Jan 1998

The Role Of Pit1 In The Putative Quantitative Trait Loci Region On Pig Chromosome 13 , Tun-Ping Yu

Retrospective Theses and Dissertations

PIT1 is an essential regulatory gene of growth hormone (GH), prolactin (PRL) and thyrotropin beta subunit. Previously, PIT1 polymorphisms and the chromosomal region near PIT1 in multiple pig populations is significantly associated with fatness and growth quantitative trait loci (QTLs) on pig chromosome 13. The purpose of this study is to confirm these previous results and clarify the role of the PIT1 gene in the putative QTL region;Cloning of the full length PIT1 cDNA was completed. The pig PIT1 cDNA and its deduced amino acids have approximately 90% and 95% sequence similarity respectively with the PIT1 cDNA and amino ...


An Integrated Multiple Capillary Array Electrophoresis System For High-Throughput Dna Sequencing , Xiandan Lu Jan 1998

An Integrated Multiple Capillary Array Electrophoresis System For High-Throughput Dna Sequencing , Xiandan Lu

Retrospective Theses and Dissertations

Capillary array electrophoresis system was chosen to perform DNA sequencing because of several advantages such as rapid heat dissipation, multiplexing capabilities, gel matrix filling simplicity, and the mature nature of the associated manufacturing technologies;There are two major concerns for the multiple capillary systems. One concern is inter-capillary cross-talk and the other concern is excitation and detection efficiency. Cross-talk is eliminated through proper optical coupling, good focusing and immersing capillary array into index matching fluid. A side-entry excitation scheme with orthogonal detection was established for large capillary array. Two 100 capillary array formats were used for DNA sequencing. One format ...


Investigating The Mechanism Of Mrp/Plf Gene Expression By Bfgf , Alesia Marie Hruska-Hageman Jan 1998

Investigating The Mechanism Of Mrp/Plf Gene Expression By Bfgf , Alesia Marie Hruska-Hageman

Retrospective Theses and Dissertations

The mitogen-regulated protein/proliferin (mrp/ plf) genes belong to the prolactin/growth hormone gene superfamily and encode at least four closely related proteins. Identified functions of these proteins include stimulation of uterine proliferation and endothelial angiogenesis. In 3T3 cells, basic fibroblast growth factor (bFGF) stimulates the production of mrp/plf mRNAs with a resulting increase in the protein products. Although the three cloned mrp/ plf gene promoters (mrp3, plf42, and plf149 ) are over 97% identical in sequence, only mrp3 is transcriptionally activated by bFGF. We have identified a sequence in the mrp3 promoter, which we have named the "bFGF-responsive element ...


Biophysical Studies Of Catalytic And Starch Binding Domains Of Wild-Type And Mutant Glucoamylases From Aspergillus Awamori , Saber Malek Abdullah Khan Jan 1998

Biophysical Studies Of Catalytic And Starch Binding Domains Of Wild-Type And Mutant Glucoamylases From Aspergillus Awamori , Saber Malek Abdullah Khan

Retrospective Theses and Dissertations

Aspergillus awamori catalytic and starch binding domains were prepared, purified and characterized. Sedimentation equilibrium experiments and MALDI-TOF spectra reveals that GA470 and SBD contains ~12% and 5% carbohydrate, respectively. Temperature denaturation experiments indicate that catalytic domain unfolds irreversibly whereas that of SBD is reversible with DeltaH of ~410 and 71 kcal/mol, respectively, and T m of 60.9° and 51.3°C, respectively. Analysis of scan rate dependency data suggest that the thermal unfolding of GA470 was partially under kinetic control and do not follow a single two-step model. Comparison of Tm of isolated and intact GA470 and SBD ...