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Full-Text Articles in Molecular Biology

An Enzymology And Structural Biology Approach Towards Elucidation Of E. Coli Fatty Acid Biosynthesis And Its Application To The Production Of Bio-Renewables, Aaron Michael Marcella Jan 2017

An Enzymology And Structural Biology Approach Towards Elucidation Of E. Coli Fatty Acid Biosynthesis And Its Application To The Production Of Bio-Renewables, Aaron Michael Marcella

Graduate Theses and Dissertations

Prokaryotic fatty acid biosynthesis is an important process to study due to its necessity for cell survival and production of highly reduced carbon chains. A characterization of several proteins involved in E. coli fatty acid biosynthesis is presented. This work provides a method for generating large amounts of pure malonyl acyl-carrier-protein using both the wild type malonyl-CoA:holo-ACP transacylase (FabD) and a variant studied further in our lab. This preparation overcomes the phenomena of a 50:50 mixture of malonyl and acetyl-ACP in the reaction and is crucial for the efficacy of future assays involving malonyl-ACP. A new assay has ...


Investigations Into Nutritional Quality, Commercial Productivity, And Genetic Purity Of Maize Grain For Organic Production Systems, Ryan Huffman Jan 2017

Investigations Into Nutritional Quality, Commercial Productivity, And Genetic Purity Of Maize Grain For Organic Production Systems, Ryan Huffman

Graduate Theses and Dissertations

Consumer demand for organic maize grain has steadily increased in the past decade which has resulted in an increased interest for this market class by plant breeders and geneticist. Each chapter in this dissertation investigates areas of concern to the organic community and possible solutions for improvement. Chapter 2 seeks to understand which combinations of genetic mechanisms are capable of a further increase in the methionine concentration of maize grain over individual mechanisms. Based on the genetic mechanisms evaluated, it was concluded that crosses combining dzr1 and recurrent selection in hybrid combination can elevate methionine concentration and overall grain nutritional ...


Insights Into Retinal Cell Fate Determination In Vertebrates Using Transcriptomic Profiling And Genome Editing, Rebecca Chowdhury Jan 2017

Insights Into Retinal Cell Fate Determination In Vertebrates Using Transcriptomic Profiling And Genome Editing, Rebecca Chowdhury

Graduate Theses and Dissertations

Deciphering the mechanisms of development of retinal neurons is not only of immense interest to developmental biologists, but is also vital for regenerative therapeutic applications. To attain this goal, it is critical to understand how specific intrinsic factors control cell fate decisions and neuronal maturation processes. In the retina, Atoh7 is a highly conserved transcription factor that is essential for retinal ganglion cell development in the developing mouse and zebrafish. Atoh7 labels a subset of cells in the developing retina that are progressing from a progenitor to a differentiated state. To capture cells during the window when the cell fate ...


Mitochondrial Heteroplasmy Contributes To The Dynamic Atovaquone Resistance Response In Plasmodium Falciparum, Sasha Victoria Siegel Nov 2016

Mitochondrial Heteroplasmy Contributes To The Dynamic Atovaquone Resistance Response In Plasmodium Falciparum, Sasha Victoria Siegel

Graduate Theses and Dissertations

Of the considerable challenges researchers face in the control and elimination of malaria, the development of antimalarial drug resistance in parasite populations remains a significant hurdle to progress worldwide. Atovaquone is used in combination with proguanil (Malarone) as an antimalarial treatment in uncomplicated malaria, but is rendered ineffective by the rapid development of atovaquone resistance during treatment. Previous studies have established that de novo mutant parasites confer resistance to atovaquone with a substitution in amino acid 268 in the cytochrome b gene encoded by the parasite mitochondrial genome, yet much is still unknown about how this resistance develops, and whether ...


The Effects Of Phytohormones And Isoprenoids In Dihydroartemisinin-Induced Dormancy In The Erythrocytic Stages Of Plasmodium Falciparum, Marvin Duvalsaint Duvalsaint Nov 2016

The Effects Of Phytohormones And Isoprenoids In Dihydroartemisinin-Induced Dormancy In The Erythrocytic Stages Of Plasmodium Falciparum, Marvin Duvalsaint Duvalsaint

Graduate Theses and Dissertations

Our ability to control malaria has been challenged by increasing antimalarial resistance. Plasmodium falciparum undergoes dormancy in the blood stages which is hypothesized to be a means by which they are able to survive under drug pressure. This helps select for resistant parasites which grow following removal of drug. The mechanisms behind dormancy and the subsequent recrudescence are not fully understood but translating knowledge from related organisms which undergo a similar phenomenon might shed some light. Higher plants utilize dormancy during the early development stages to survive under unfavorable conditions, increasing fitness of the seedling and ensuring viability when this ...


Gene Expression Profiling And The Role Of Hsf1 In Ovarian Cancer In 3d Spheroid Models, Trillitye Paullin Nov 2016

Gene Expression Profiling And The Role Of Hsf1 In Ovarian Cancer In 3d Spheroid Models, Trillitye Paullin

Graduate Theses and Dissertations

Ovarian cancer is the most lethal gynecological cancer, with over 200,000 women diagnosed each year and over half of those cases leading to death. These poor statistics are related to a lack of early symptoms and inadequate screening techniques. This results in the cancer going undetected until later stages when the tumor has metastasized through a process that requires the epithelial to mesenchymal transition (EMT). In lieu of traditional monolayer cell culture, EMT and cancer progression in general is best characterized through the use of 3D spheroid models. In this study, we examine gene expression changes through microarray analysis ...


Significance Of Pten Phosphorylation And Its Nuclear Function In Lung Cancer, Prerna Malaney Nov 2016

Significance Of Pten Phosphorylation And Its Nuclear Function In Lung Cancer, Prerna Malaney

Graduate Theses and Dissertations

Phosphorylation mediated inactivation of PTEN leads to multiple malignancies with increased severity. However, the consequence of such inactivation on downstream functions of PTEN are poorly understood. Therefore, the objective of my thesis is to ascertain the molecular mechanisms by which PTEN phosphorylation drives lung cancer. PTEN phosphorylation at the C-terminal serine/threonine cluster abrogates its tumor suppressor function. Despite the critical role of the PTEN C-tail in regulating its function, the crystal structure of the C-tail remains unknown. Using bioinformatics and structural analysis, I determined that the PTEN C-tail is an intrinsically disordered region and is a hot spot for ...


Contribution Of Epithelial Hypoxia Signaling To Pulmonary Fibrosis: Role Of Fak1 And Galectin-1 As Driver Molecules, Jaymin J. Kathiriya Oct 2016

Contribution Of Epithelial Hypoxia Signaling To Pulmonary Fibrosis: Role Of Fak1 And Galectin-1 As Driver Molecules, Jaymin J. Kathiriya

Graduate Theses and Dissertations

Idiopathic Pulmonary Fibrosis (IPF) is a deadly disease of unknown origin, which causes 80,000 deaths every year in the US and Europe combined. Unknown etiology and late diagnosis, combined with limited treatment options, contribute to a dismal survival rate of 3-5 years post diagnosis. Although molecular mechanisms underlying IPF pathogenesis and progression have been studied for over two decades, lack of in vivo models that recapitulate chronic, progressive, and irreversible nature of IPF have contributed to limited therapeutic success in clinical trials. Currently, only two drugs, Pirfenidone and Nintedanib, are approved for IPF treatment in the US, with their ...


Cardiovascular Regulation By Kvβ1.1 Subunit, Jared Tur Oct 2016

Cardiovascular Regulation By Kvβ1.1 Subunit, Jared Tur

Graduate Theses and Dissertations

Heterologous expression systems such as COS-7 cells have demonstrated the profound effects of KCNAB1-3 or Kvβ1-3 proteins on voltage gated potassium channels (Kv) channels. Indeed, in the presence of these β-subunits transiently expressed Kv channels are often modulated in multiple ways. Kv channel membrane expression is often increased in the presence of β-subunits. In addition, non-inactivating Kv currents suddenly become fast-inactivating and fast-inactivating channels become even faster. While much research has demonstrated the profound effects the β-subunits in particular the Kvβ1 subunit have on transiently expressed Kv currents little to date is known of the physiological role it may play ...


Regulation Of Nachrs And Stemness By Nicotine And E-Cigarettes In Nsclc, Courtney Schaal Aug 2016

Regulation Of Nachrs And Stemness By Nicotine And E-Cigarettes In Nsclc, Courtney Schaal

Graduate Theses and Dissertations

Lung cancer is the leading cause of cancer-related death in both men and women, nationally and internationally and kills more people each year than breast, prostate, and colon cancers combined. Non-small cell lung carcinoma (NSCLC) is the most common histological subtype of lung cancer, and accounts for 85% of all cases. Cigarette smoking is the single greatest risk factor for lung cancer, and is correlated with 80-90% of all lung cancer deaths. Nicotine, the addictive component of tobacco smoke, is not a carcinogen and cannot initiate tumors itself; however, it is known to act as a tumor promoter, by enhancing ...


Computational Modeling Of Allosteric Stimulation Of Nipah Virus Host Binding Protein, Priyanka Dutta Jul 2016

Computational Modeling Of Allosteric Stimulation Of Nipah Virus Host Binding Protein, Priyanka Dutta

Graduate Theses and Dissertations

Nipah belongs to the family of paramyxoviruses that cause numerous fatal diseases in humans and farm animals. There are no FDA approved drugs for Nipah or any of the paramyxoviruses. Designing antiviral therapies that are more resistant to viral mutations require understanding of molecular details underlying infection. This dissertation focuses on obtaining molecular insights into the very first step of infection by Nipah. Such details, in fact, remain unknown for all paramyxoviruses. Infection begins with the allosteric stimulation of Nipah virus host binding protein by host cell receptors. Understanding molecular details of this stimulation process have been challenging mainly because ...


A Caenorhabditis Elegans Model To Detect Developmental Teratogens With Adverse Reproductive And Metabolic Effects, Alexis Ann Killeen Jul 2016

A Caenorhabditis Elegans Model To Detect Developmental Teratogens With Adverse Reproductive And Metabolic Effects, Alexis Ann Killeen

Graduate Theses and Dissertations

Obesity is a current epidemic in the United States. Greater than one third of adults and nearly one fifth of children are classified as obese (Ogden, 2014). Increased awareness of this problem has triggered interest into the potentially causal agents in our environment. These agents, termed obesogens, are a type of teratogen hypothesized to work during embryonic development by programming offspring to store excess fat. A type of teratogen, obesogens are also typically reprotoxic, affecting germline development and embryonic viability. These noxious teratogens span a variety of different chemical classes including estrogen mimics, metals and metalloids, biocides, and even voluntary ...


Cell Cycle Arrest By Tgfß1 Is Dependent On The Inhibition Of Cmg Helicase Assembly And Activation, Brook Samuel Nepon-Sixt Jun 2016

Cell Cycle Arrest By Tgfß1 Is Dependent On The Inhibition Of Cmg Helicase Assembly And Activation, Brook Samuel Nepon-Sixt

Graduate Theses and Dissertations

Tumorigenesis is a multifaceted set of events consisting of the deregulation of several cell-autonomous and tissue microenvironmental processes that ultimately leads to the acquisition of malignant disease. Transforming growth factor beta (TGFß) and its family members are regulatory cytokines that function to ensure proper organismal development and the maintenance of homeostasis by controlling cellular differentiation, proliferation, adhesion, and survival, as well as by modulating components of the cellular microenvironment and immune system. The pleiotropic control by TGFß of these cell intrinsic and extrinsic factors is intimately linked to the prevention of tumor formation, the specifics of which are dependent on ...


Intrinsic Disorder Where You Least Expect It: The Incidence And Functional Relevance Of Intrinsic Disorder In Enzymes And The Protein Data Bank, Shelly Deforte Jun 2016

Intrinsic Disorder Where You Least Expect It: The Incidence And Functional Relevance Of Intrinsic Disorder In Enzymes And The Protein Data Bank, Shelly Deforte

Graduate Theses and Dissertations

Intrinsically disordered proteins (IDPs) and intrinsically disordered protein regions (IDPRs) exist as interconverting conformational ensembles, without a single fixed three-dimensional structure in vivo. The focus in the literature up to this point has been primarily on IDPs that are mostly or entirely disordered. Therefore, we have an incomplete understanding of the incidence and functional relevance of IDPRs in proteins that have regions of both order and disorder. This work explores these populations, by examining IDPRs in the Protein Data Bank (PDB) and in enzymes. By applying disorder prediction methods combined with an analysis of missing regions in crystal structure data ...


Inhibiting The Interaction Between Grp94 And Myocilin To Treat Primary Open-Angle Glaucoma, Andrew Stothert Jun 2016

Inhibiting The Interaction Between Grp94 And Myocilin To Treat Primary Open-Angle Glaucoma, Andrew Stothert

Graduate Theses and Dissertations

Glaucoma is a neurodegenerative protein misfolding disorder classified by increases in IOP, damage to retinal ganglion cells (RGCs), optic nerve (ON) head damage, and progressive irreversible blindness. Primary open-angle glaucoma (POAG) is the most common form of glaucoma, constituting over 90% of clinical cases. POAG is observed in patients where normal outflow channels, mainly the trabecular meshwork (TM), are exposed at the angle formed by the iris and cornea. However, due to TM cellular dysfunction, aqueous outflow resistance is increased preventing normal circulation of aqueous humor. Recent studies have shown that in 2-4% of POAG cases, increased intracellular levels of ...


Cmg Helicase Assembly And Activation: Regulation By C-Myc Through Chromatin Decondensation And Novel Therapeutic Avenues For Cancer Treatment, Victoria Bryant Jun 2016

Cmg Helicase Assembly And Activation: Regulation By C-Myc Through Chromatin Decondensation And Novel Therapeutic Avenues For Cancer Treatment, Victoria Bryant

Graduate Theses and Dissertations

The CMG (Cdc45, MCM, GINS) helicase is required for cellular proliferation and functions to unwind double-stranded DNA to allow the replication machinery to duplicate the genome. Cancer cells mismanage helicase activation through a variety of mechanisms, leading to the potential for the development of novel anti-cancer treatments. Mammalian cells load an excess of MCM complexes that act as reserves for new replication origins to be created when replication forks stall due to stress conditions, such as drug treatment. Targeting the helicase through inhibition of the MCM complex has sensitized cancer cells to drugs that inhibit DNA replication, such as aphidicolin ...


Investigation Of Respiratory Syncytial Virus Structural Determinants And Exploitation Of The Host Ubiquitin System, Jillian Nicole Whelan Apr 2016

Investigation Of Respiratory Syncytial Virus Structural Determinants And Exploitation Of The Host Ubiquitin System, Jillian Nicole Whelan

Graduate Theses and Dissertations

Respiratory syncytial virus (RSV) is a globally circulating, non-segmented, negative sense (NNS) RNA virus that causes severe lower respiratory infections. This study explored several avenues to ultimately expand upon our understanding of RSV pathogenesis at the protein level. Evaluation of RSV intrinsic protein disorder increased the relatively limited description of the RSV structure-function relationship. Global proteomics analysis provided direction for further hypothesis-driven investigation of host pathways altered by RSV infection, specifically the interaction between the RSV NS2 protein and the host ubiquitin system. NS2 primarily acts to antagonize the innate immune system by targeting STAT2 for proteasomal degradation. The goal ...


Nonreplicative Dna Helicases Involved In Maintaining Genome Stability, Salahuddin Syed Apr 2016

Nonreplicative Dna Helicases Involved In Maintaining Genome Stability, Salahuddin Syed

Graduate Theses and Dissertations

Double-strand breaks and stalled forks arise when the replication machinery encounters damage from exogenous sources like DNA damaging agents or ionizing radiation, and require specific DNA helicases to resolve these structures. Sgs1 of Saccharomyces cerevisiae is a member of the RecQ family of DNA helicases and has a role in DNA repair and recombination. The RecQ family includes human genes BLM, WRN, RECQL4, RECQL1, and RECQL5. Mutations in BLM, WRN, and RECQL4 result in genetic disorders characterized by developmental abnormalities and a predisposition to cancer. All RecQ helicases have common features including a helicase domain, an RQC domain, and a ...


In Vitro And In Vivo Antioxidant Capacity Of Synthetic And Natural Polyphenolic Compounds Identified From Strawberry And Fruit Juices, Marvin Abountiolas Mar 2016

In Vitro And In Vivo Antioxidant Capacity Of Synthetic And Natural Polyphenolic Compounds Identified From Strawberry And Fruit Juices, Marvin Abountiolas

Graduate Theses and Dissertations

Strawberries can be considered a functional food because their consumption has been associated with several health benefits. They are important sources of bioactive compounds, such as vitamins and polyphenolic compounds, with recognized antioxidant capacity (AOC). However, strawberry overall quality and bioactive content are greatly affected by environmental conditions during pre- and post-harvest and, little is known about the stability of its bioactive compounds, specifically ascorbic acid (AA) and polyphenolics compounds. Furthermore, additional research that addresses the impact of polyphenolic compounds on in vitro and in vivo models is needed to understand the mechanisms behind their potential health benefits. Therefore, the ...


Analysis Of The Induction Of Autophagy During Er Stress And The Vesicle Fusion Machinery At The Trans-Golgi Network In Arabidopsis Thaliana, Xiaochen Yang Jan 2016

Analysis Of The Induction Of Autophagy During Er Stress And The Vesicle Fusion Machinery At The Trans-Golgi Network In Arabidopsis Thaliana, Xiaochen Yang

Graduate Theses and Dissertations

The vacuole in plant cells occupies more than 80% of the cellular volume and is involved in development, detoxification, and degradation of proteins. In this dissertation, I focused on the two vesicle trafficking pathways, autophagy and vacuolar trafficking, which deliver cellular components to the vacuole.

Autophagy is an evolutionarily conserved mechanism in eukaryotic cells for degradation and recycling of cytoplasmic materials and damaged cell components during development or upon encountering stress conditions. Previous studies showed that autophagy is activated by endoplasmic reticulum (ER) stress and delivers ER fragments to the vacuole. ER stress is defined as accumulation of unfolded or ...


Strep-Tag Ii Fusion Technology For The Immobilization Of Lipase B From Candida Antarctica, Sumreet Singh Johar Jan 2016

Strep-Tag Ii Fusion Technology For The Immobilization Of Lipase B From Candida Antarctica, Sumreet Singh Johar

Graduate Theses and Dissertations

Fusion tags are genetically coded protein or peptides that can be expressed as attached moieties to the desired enzyme to improve downstream purification of the target protein. Fusion tags have the potential to provide specific immobilization with repeatable attachment of the enzyme, eliminate the need for purification and post-modification and enable dissociation of spent enzyme and regeneration of the support. Lipase is an industry-relevant enzyme utilized for the production of natural flavors, biodegradable polyesters for food packaging, structured lipids, antioxidant esters, sugar esters and several other important compounds used in food and agriculture industries. In this research work, we are ...


Introducing Multiple Sites Of Acetylation To Histone H3 Via Nonsense Suppression, Isaac Young Jan 2016

Introducing Multiple Sites Of Acetylation To Histone H3 Via Nonsense Suppression, Isaac Young

Graduate Theses and Dissertations

A common post-translational modification (PTM) of proteins is lysine acetylation. This is an especially ubiquitous PTM in the histones of chromatin, and is important for helping to regulate both structural and mechanistic aspects of chromatin. The fundamental unit of chromatin is called the nucleosome and is made up of DNA that wraps around a histone protein octamer. Protruding from the nucleosome are 10 unstructured “tails” which protrude into the aqueous environment. A number of strategies exist for generating acetylated nucleosomes for the in-vitro study of chromatin including: Purification from eukaryotic organisms, chemical acetylation, amino acid analog incorporation, enzyme mediated acetylation ...


Investigation Of Snare Mediated Membrane Fusion And Its Regulation By Optimized Single Molecule Method, Yicheng Zhu Jan 2016

Investigation Of Snare Mediated Membrane Fusion And Its Regulation By Optimized Single Molecule Method, Yicheng Zhu

Graduate Theses and Dissertations

Neurotransmitter release going through synaptic vesicle cycle is one key step how signal is transported in our brains. The mechanism on molecular level has been under development and debated for decades. Many milestones have been made including, the identification of SNARE as core assembly machinery, the clarification of synaptotagmin as calcium sensor, the recognition of NSF and SNAP as disassembly apparatus, the determination of complexin and SM protein as regulatory protein. However, the sequence of their involvement in synaptic vesicle cycle, the relationship between the structure and psychological function, microscale fusion mechanism and are under further investigation. This puzzle is ...


Role Of Non-Catalytic Ligands In Macromolecule Function, Muneaki Watanabe Jan 2016

Role Of Non-Catalytic Ligands In Macromolecule Function, Muneaki Watanabe

Graduate Theses and Dissertations

Human hexokinase Type-I (HKI) binds to the outer mitochondrial membrane, and in so doing protects the mitochondrion, controls adenine nucleotide flux through the membrane and blocks mitochondrion-linked apoptosis. Adenosine 5’-triphosphate (ATP) releases HKI from the outer mitochondrial membrane, but the mechanism of ATP release is unclear. ATP-release is not due to the generation of glucose 6-phosphate (G6P) from ATP and glucose or by the binding of ATP to HKI at its catalytic or regulatory domains. Instead, the voltage dependent anion channel (VDAC) has a high affinity binding site for ATP as revealed by titrations using ATP and fluorescent analogs ...


On The Molecular Biology And Evolution Of Plant Parasitism By Nematodes, Jason Brett Noon Jan 2016

On The Molecular Biology And Evolution Of Plant Parasitism By Nematodes, Jason Brett Noon

Graduate Theses and Dissertations

Plant-parasitic nematodes (PPN) are among the most devastating plant pathogens. However, our understanding of how nematodes adapted to plant parasitism, and the molecular mechanisms that PPN use during infection is limited. Among the most important genomic changes that occurred in the free-living nematode ancestors of PPN were multiple horizontal gene transfer (HGT) events from bacteria. Though it is clear that HGT helped shape the genomes of many PPN, how this process occurred is unknown. Also, it is evident that successful parasitism occurs from the delivery of proteinaceous effectors into plant roots to hijack and modify host cellular processes.

The research ...


Characterization Of P73 And Stat5b Genes That Are Susceptible To Manganese Exposure In Dopaminergic Neurons, Dongsuk Kim Jan 2016

Characterization Of P73 And Stat5b Genes That Are Susceptible To Manganese Exposure In Dopaminergic Neurons, Dongsuk Kim

Graduate Theses and Dissertations

Manganese (Mn) is an essential trace element found in most living organisms. Chronic exposure to Mn has been linked to the pathogenesis of manganism, which displays neurological abnormalities somewhat similar to those associated with Parkinson's disease resulting from dysfunction of the extrapyramidal motor system within the basal ganglia. However, the exact cellular and molecular mechanisms underlying Mn induced neurotoxicity have not been defined. Oxidative stress mediated dopaminergic neuronal apoptosis is considered to be the prime mechanisms of Mn neurotoxicity. Thus, we sought to identify the genes that are altered during Mn exposure and that lead us to elucidate the ...


Novel Functions Of The Survival Motor Neuron Protein, Eric William Ottesen Jan 2016

Novel Functions Of The Survival Motor Neuron Protein, Eric William Ottesen

Graduate Theses and Dissertations

The Survival Motor Neuron (SMN) protein is a multi-functional protein that participates in a wide variety of critical pathways. Low levels of SMN cause spinal muscular atrophy (SMA), the most common genetic cause of infant mortality. While the role of SMN in the assembly of small nuclear ribonucleoproteins (snRNPs) has been well characterized, many of its other diverse functions have not been thoroughly explored. Here, we examine the critical role of SMN in the growth and development of male mammalian sex organs. We show that low levels of SMN in a mild mouse model of SMA cause impaired testis development ...


Exploring The Genetic Redundancy Of Zea Mays Fatty Acid Elongase (Fae), Kayla S. Flyckt Jan 2016

Exploring The Genetic Redundancy Of Zea Mays Fatty Acid Elongase (Fae), Kayla S. Flyckt

Graduate Theses and Dissertations

Very-long-chain fatty acids (VLCFAs) consist of greater than 18 carbon atoms and have diverse biological functions including energy storage, protection from biotic and abiotic stresses, signaling, and are components of the plasma membrane. The biosynthesis of VLCFAs occurs by the iterative extension of fatty acyl-CoAs through a series of reactions catalyzed by four enzyme components known as fatty acid elongase (FAE). The FAE enzyme 3-ketoacyl CoA synthase (KCS) is hypothesized to be responsible for the substrate specificity of FAE, controlling the amount and identity of VLCFAs produced in specific cell types and tissues. The KCS gene family consists of multiple ...


Mechanisms Of Communication In The Filarial Worm, Brugia Malayi, At The Mosquito Vector-Mammalian Host Interface, Lisa Marie Fraser Jan 2016

Mechanisms Of Communication In The Filarial Worm, Brugia Malayi, At The Mosquito Vector-Mammalian Host Interface, Lisa Marie Fraser

Graduate Theses and Dissertations

Lymphatic Filariasis (LF) is a Neglected Tropical Disease, caused by mosquito-borne filarial nematodes including Brugia malayi, which infects over 120 million people. Mass drug administration is used for LF control but limited efficacy of the drugs combined with the threat of resistance drives the need for novel control strategies. Chemosensation is an essential behavior used by nematodes, including parasitic species, has roles in development, avoidance of noxious stimuli and finding food/mates/hosts, making genes involved in this system attractive targets for parasite control. Little, however, is known about the chemosensory system in parasitic nematodes. Here we address this knowledge ...


Prokineticin-2: A Novel Anti-Apoptotic And Anti-Inflammatory Signaling Protein In Parkinson’S Disease, Matthew Neal Jan 2016

Prokineticin-2: A Novel Anti-Apoptotic And Anti-Inflammatory Signaling Protein In Parkinson’S Disease, Matthew Neal

Graduate Theses and Dissertations

The mechanisms behind the development and progression of Parkinson’s disease (PD) are still not fully understood. Several pathophysiological mechanisms have been implicated in the progression of the disease including oxidative stress, mitochondrial dysfunction, excitotoxicity, protein aggregation, and neuroinflammation. Since current therapeutic options for PD treat only the symptoms without influencing disease progression, recent translational studies have focused on identifying factors that protect against putative pathophysiological mechanisms of PD. We show herein, that the chemokine-like signaling protein, Prokineticin-2 (PK2), is expressed and secreted at higher levels in dopaminergic neurons during the acute phase of inflammatory or neurotoxic stress. Furthermore, we ...