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Theses/Dissertations

Graduate Theses and Dissertations

2014

EHEC

Discipline

Articles 1 - 2 of 2

Full-Text Articles in Molecular Biology

Genetic Basis For The Virulence Of Enterohemorrhagic Escherichia Coli Strain Tw14359, Jason Kyle Morgan May 2014

Genetic Basis For The Virulence Of Enterohemorrhagic Escherichia Coli Strain Tw14359, Jason Kyle Morgan

Graduate Theses and Dissertations

Enterohemorrhagic Escherichia coli (EHEC) is a virulent pathotype of E. coli that is associated with major outbreaks of hemorrhagic colitis and the life-threatening kidney disease hemolytic uremic syndrome. For successful host colonization and attachment to the intestinal mucosa, EHEC requires the locus of enterocyte effacement (LEE) pathogenicity island, which encodes a type III secretion system (TTSS) responsible for secreting and translocating effector proteins into host colonocytes. Regulation of the LEE is primarily directed through the first operon, LEE1, encoding the locus encoded regulator (Ler), and occurs through the direct and indirect action of several regulators. The 2006 U.S. spinach ...


Sigma Factor N: A Novel Regulator Of Acid Resistance And Locus Of Enterocyte Effacement In Escherichia Coli O157:H7, Avishek Mitra Mar 2014

Sigma Factor N: A Novel Regulator Of Acid Resistance And Locus Of Enterocyte Effacement In Escherichia Coli O157:H7, Avishek Mitra

Graduate Theses and Dissertations

In enterohemorrhagic E. coli (EHEC) sigma factor N (σN) regulates glutamate-dependent acid resistance (GDAR) and the locus of enterocyte effacement (LEE), discrete genetic systems required for transmission and virulence of this intestinal pathogen. Regulation of these systems requires nitrogen regulatory protein C, NtrC, and is a consequence of NtrC/σN-dependent reduction in the activity of sigma factor S (σS). This study elucidates pathway components and stimuli for σN-directed regulation of GDAR and the LEE in EHEC. Deletion of fliZ, the product of which reduces σS activity, phenocopies rpoNN) and ntrC null ...