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Full-Text Articles in Molecular Biology

Identification And Characterization Of A Novel Structural Protein Of Porcine Reproductive And Respiratory Syndrome Virus, The Replicase Nonstructural Protein 2, Matthew Allan Kappes Jan 2014

Identification And Characterization Of A Novel Structural Protein Of Porcine Reproductive And Respiratory Syndrome Virus, The Replicase Nonstructural Protein 2, Matthew Allan Kappes

Graduate Theses and Dissertations

Porcine reproductive and respiratory syndrome virus (PRRSV) is a rapidly mutating pathogen eliciting respiratory and reproductive disease of high economic consequence. The PRRSV non-structural protein 2 (nsp2) is a large multifunctional protein encoded by the most genetically diverse region of the genome - the selective pressure potentiating mutation within this region is unknown. Here we report the identification of a unique function of nsp2 as a structural component of the PRRSV virion; the first PRRSV structural protein identified which is not expressed from a sub-genomic RNA or regulated via the discontinuous transcription pathway. Through the use of a set of custom ...


Aphid Small Rnas And Viruses, Diveena Vijayendran Jan 2014

Aphid Small Rnas And Viruses, Diveena Vijayendran

Graduate Theses and Dissertations

Aphids are important agricultural pests with about 250 species known to cause damage by feeding on plant phloem and transmitting plant viruses. Current management relies primarily on the application of chemical insecticides, which can be deleterious to the environment, and the use of aphid resistant cultivars hampered by the presence of resistant aphid biotypes. We aim to develop novel biotechnology based approaches to better manage pestiferous aphids. To this end, we (i) identified and characterized aphid viruses, and (ii) analyzed a class of small RNA, microRNAs specific to aphids. We used transcriptome and small RNA sequencing datasets to identify viruses ...