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Full-Text Articles in Molecular Biology

Comparison And Evaluation Of Two Rapid Diagnostic Assays For The Identification Of Mycoplasma Species, Aric James Mcdaniel Jan 2018

Comparison And Evaluation Of Two Rapid Diagnostic Assays For The Identification Of Mycoplasma Species, Aric James Mcdaniel

Graduate Theses and Dissertations

Members of the class Mollicutes are unique among bacteria in that they are unable to synthesize a cell wall. These organisms are also difficult to culture and very slow-growing with some species requiring up to 40 days to culture. Many of these bacteria are also significant pathogens in humans and animals, so rapid identification of isolates is a necessary diagnostic step. Many different tests are used for identification of pathogenic Mollicutes (primarily Mycoplasma and Ureaplasma species), however most tests are not cost or time efficient. Two relatively recent diagnostic tools are matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry and ...


Microbial Community Diversity In Freshwater Sponges From Lake Baikal/Protein Size In Three Domains Of Life And Examples Of Unusually Large Proteins In Porifera: A Review, Katrina Agno Lutap Jan 2015

Microbial Community Diversity In Freshwater Sponges From Lake Baikal/Protein Size In Three Domains Of Life And Examples Of Unusually Large Proteins In Porifera: A Review, Katrina Agno Lutap

Graduate Theses and Dissertations

Sponges (phylum Porifera) are sessile, filter-feeding animals that are found in both marine and freshwater environments. They are known to be associated with highly diverse and stable microbial communities. The first component of this thesis presents a study on the diversity of the microbial communities associated with freshwater sponges collected from Lake Baikal, Baikalospongia intermedia profundalis (BIP) and Lubomirskia baicalensis (LB). Based on previous studies, microbial communities associated with freshwater sponges are not as diverse as those from marine sponges. However, one of the potential reasons for this observation is the limitation posed by conventional cloning and sequencing methods used ...


Engineering Cry4aa For Toxicity Against The Soybean Aphid, Aphis Glycines Matsumura, Benjamin Robert Deist Jan 2015

Engineering Cry4aa For Toxicity Against The Soybean Aphid, Aphis Glycines Matsumura, Benjamin Robert Deist

Graduate Theses and Dissertations

Soybean aphid poses a significant threat to soybean agriculture in North America. Current management strategies are heavily dependent on chemical insecticides, which while presently effective, may have negative side effects. Bt toxins have been successfully used in various transgenic crops for almost 20 years to manage lepidopteran and coleopteran pests. However, this strategy has not been used in the management of aphids and other hemipterans due to their low susceptibility to Bt toxins, potentially arising from a lack of binding to the hemipteran gut. To circumvent this limitation in soybean aphids, a phage display library was screened in vivo against ...


Development Of An In Vitro Model And Fluorescent Protein Expression System For The Study Of Highly Abortigenic Campylobacter Jejuni, Moria Ann Borys Jan 2014

Development Of An In Vitro Model And Fluorescent Protein Expression System For The Study Of Highly Abortigenic Campylobacter Jejuni, Moria Ann Borys

Graduate Theses and Dissertations

Campylobacter spp. are a leading cause of sheep abortions worldwide; Campylobacter fetus ssp. fetus has historically been the major culprit. Increasingly, however, C. jejuni has been isolated from cases of sheep abortion, and it has now replaced C. fetus ssp. fetus as the predominant cause of Campylobacter-related ovine abortion in the United States. Emergence of a single tetracycline resistant clone (clone SA) has been implicated as the primary reason for this shift. Virulence factors for clone SA have still not been completely elucidated, and it is not known how this bacterium reaches the uterine and placental tissue from the ...


Identification And Characterization Of A Novel Structural Protein Of Porcine Reproductive And Respiratory Syndrome Virus, The Replicase Nonstructural Protein 2, Matthew Allan Kappes Jan 2014

Identification And Characterization Of A Novel Structural Protein Of Porcine Reproductive And Respiratory Syndrome Virus, The Replicase Nonstructural Protein 2, Matthew Allan Kappes

Graduate Theses and Dissertations

Porcine reproductive and respiratory syndrome virus (PRRSV) is a rapidly mutating pathogen eliciting respiratory and reproductive disease of high economic consequence. The PRRSV non-structural protein 2 (nsp2) is a large multifunctional protein encoded by the most genetically diverse region of the genome - the selective pressure potentiating mutation within this region is unknown. Here we report the identification of a unique function of nsp2 as a structural component of the PRRSV virion; the first PRRSV structural protein identified which is not expressed from a sub-genomic RNA or regulated via the discontinuous transcription pathway. Through the use of a set of custom ...


Aphid Small Rnas And Viruses, Diveena Vijayendran Jan 2014

Aphid Small Rnas And Viruses, Diveena Vijayendran

Graduate Theses and Dissertations

Aphids are important agricultural pests with about 250 species known to cause damage by feeding on plant phloem and transmitting plant viruses. Current management relies primarily on the application of chemical insecticides, which can be deleterious to the environment, and the use of aphid resistant cultivars hampered by the presence of resistant aphid biotypes. We aim to develop novel biotechnology based approaches to better manage pestiferous aphids. To this end, we (i) identified and characterized aphid viruses, and (ii) analyzed a class of small RNA, microRNAs specific to aphids. We used transcriptome and small RNA sequencing datasets to identify viruses ...


The Panicum Mosaic Virus-Like 3' Cap-Independent Translation Element: A Translation Enhancer That Functions In Mammalian Systems, Mariko Sada Peterson Jan 2013

The Panicum Mosaic Virus-Like 3' Cap-Independent Translation Element: A Translation Enhancer That Functions In Mammalian Systems, Mariko Sada Peterson

Graduate Theses and Dissertations

In canonical eukaryotic translation initiation, the recognition of mRNA by the translation machinery is facilitated through the binding of the 5' m7G cap structure to eukaryotic initiation factor 4E (eIF4E). This recognition is necessary for efficient translation of the message to occur. In contrast, some viruses lack the 5' cap structure but have alternative mechanisms to initiate translation and express the viral proteins necessary for replication. The positive-strand RNA plant viruses of the panico-, carmo-, umbra-, (1-3) and aureusviruses (4) contain an RNA structure in their 3' UTRs that binds eIF4E (2) and through communication with the 5' UTR, are ...


New Tools For Studying Mal Gene Regulation In Yersinia Pestis, Carrie Jo Oster Jan 2012

New Tools For Studying Mal Gene Regulation In Yersinia Pestis, Carrie Jo Oster

Graduate Theses and Dissertations

Y. pestis, the etiologic agent of plague, is a significant human pathogen because of its historic role as the cause of three major pandemics and its current role as a potential bioterrorism agent. Gene regulation in this organism is complex and multiple microarray studies have shown that the expression of several genes is affected by quorum sensing as well as temperature. Maltose utilization genes are of particular interest because they are regulated by both of these systems. In order to perform genetic studies on mal genes, and to better understand gene expression in general in Y. pestis, we developed a ...