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Articles 1 - 5 of 5

Full-Text Articles in Molecular Biology

Therapeutic Modulation Of Cancer Metabolism With Dichloroacetate And Metformin, Nathan Patrick Ward Apr 2017

Therapeutic Modulation Of Cancer Metabolism With Dichloroacetate And Metformin, Nathan Patrick Ward

Graduate Theses and Dissertations

The robust glycolytic metabolism of glioblastoma multiforme (GBM) has proven them susceptible to increases in oxidative metabolism induced by the pyruvate mimetic dichloroacetate (DCA). Recent reports demonstrate that the anti-diabetic drug metformin enhances the damaging oxidative stress associated with DCA treatment in cancer cells. We sought to elucidate the role of metformin’s reported activity as a mitochondrial complex I inhibitor in the enhancement of DCA cytotoxicity in the VM-M3 model of GBM. We demonstrated that metformin potentiated DCA-induced superoxide production and that this was required for enhanced cytotoxicity towards VM-M3 cells with the combination. Similarly, rotenone enhanced oxidative stress ...


A Caenorhabditis Elegans Model To Detect Developmental Teratogens With Adverse Reproductive And Metabolic Effects, Alexis Ann Killeen Jul 2016

A Caenorhabditis Elegans Model To Detect Developmental Teratogens With Adverse Reproductive And Metabolic Effects, Alexis Ann Killeen

Graduate Theses and Dissertations

Obesity is a current epidemic in the United States. Greater than one third of adults and nearly one fifth of children are classified as obese (Ogden, 2014). Increased awareness of this problem has triggered interest into the potentially causal agents in our environment. These agents, termed obesogens, are a type of teratogen hypothesized to work during embryonic development by programming offspring to store excess fat. A type of teratogen, obesogens are also typically reprotoxic, affecting germline development and embryonic viability. These noxious teratogens span a variety of different chemical classes including estrogen mimics, metals and metalloids, biocides, and even voluntary ...


Characterization Of P73 And Stat5b Genes That Are Susceptible To Manganese Exposure In Dopaminergic Neurons, Dongsuk Kim Jan 2016

Characterization Of P73 And Stat5b Genes That Are Susceptible To Manganese Exposure In Dopaminergic Neurons, Dongsuk Kim

Graduate Theses and Dissertations

Manganese (Mn) is an essential trace element found in most living organisms. Chronic exposure to Mn has been linked to the pathogenesis of manganism, which displays neurological abnormalities somewhat similar to those associated with Parkinson's disease resulting from dysfunction of the extrapyramidal motor system within the basal ganglia. However, the exact cellular and molecular mechanisms underlying Mn induced neurotoxicity have not been defined. Oxidative stress mediated dopaminergic neuronal apoptosis is considered to be the prime mechanisms of Mn neurotoxicity. Thus, we sought to identify the genes that are altered during Mn exposure and that lead us to elucidate the ...


Modification Of The Bt Toxin Cry4aa For Improved Toxin Processing In The Gut Of The Pea Aphid (Acyrthosiphon Pisum), Michael Allen Rausch Jan 2014

Modification Of The Bt Toxin Cry4aa For Improved Toxin Processing In The Gut Of The Pea Aphid (Acyrthosiphon Pisum), Michael Allen Rausch

Graduate Theses and Dissertations

Aphids are sap-sucking insects (order: Hemiptera) that cause extensive damage to a wide range of agricultural crops. Our goal is to produce a "designer" toxin based on naturally occurring insecticidal crystalline (Cry) toxins produced by the soil-dwelling bacterium Bacillus thuringiensis for use against the pea aphid, Acyrthosiphon pisum. On the basis that activation of the Cry4Aa protoxin could be a rate-limiting factor contributing to the relatively low aphicidal activity of this toxin, we introduced cathepsin L and cathepsin B cleavage sites into Cry4Aa for rapid activation and generation of the active toxin in the aphid gut environment. Incubation of modified ...


Specificity Of The Cocaine Aptamer And Its Application In Measurement Of Cocaine And Metabolites In Homogeneous Samples For Forensic Analysis, Ashish Sachan Jan 2013

Specificity Of The Cocaine Aptamer And Its Application In Measurement Of Cocaine And Metabolites In Homogeneous Samples For Forensic Analysis, Ashish Sachan

Graduate Theses and Dissertations

Detection and quantification of molecules has an enormous importance and potential in the field of scientific development and basic discovery research. Advances in colorimetric techniques, fluorescent labeled probes and biosensor based technologies have resulted in the specific detection and precise quantification of analytes. The effective development of a particular detection method needs optimization in terms of overall speed, efficiency, and accuracy of the sensing system. Current biosensor approaches often rely on cumbersome procedural steps and reagent requirements. Along with the complexity of the assay, the size of the analytical instrument is a deterrent factor for on-site applications. To overcome such ...