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Transcriptional Regulation Of Proapoptotic Kinase Pkcî´ Expression In Dopaminergic Neurons: Relevance To Gene-Gene And Gene-Environment Interactions In Neurodegeneration, Huajun Jin
Graduate Theses and Dissertations
We investigated the mechanisms of transcriptional regulation of the PKCδ gene. By deletion analysis of the ~1.4 kb (-1448 to +1, relative to transcription start site) 5'-flanking sequence of the mouse PKCδ gene, we have identified a basal promoter region, nucleotide -148 to +1, required for sufficient PKCδ transcription in NIE115, MN9D, and N2a cells. We further identified two NFκB binding sites (κB 1, κB 2) as well as a NERF1a site within the basal promoter as key regulatory elements in the mouse PKCδ TATA-less promoter. Subsequent functional studies using site-directed mutation analysis revealed that κB 1, but ...