Open Access. Powered by Scholars. Published by Universities.®

Molecular Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 4 of 4

Full-Text Articles in Molecular Biology

Evaluation Of Methods For Generating Senecavirus A Virus-Like Particles Utilizing The Baculovirus Expression Vector System, Jennifer Lynn English Jan 2017

Evaluation Of Methods For Generating Senecavirus A Virus-Like Particles Utilizing The Baculovirus Expression Vector System, Jennifer Lynn English

Graduate Theses and Dissertations

A recombinant subunit vaccine against Seneca Valley virus (SVV) would be valuable to the swine industry. Recent SVV outbreaks have raised concerns with national biosecurity. This is due to the potential of foreign vesicular animal diseases entering the United States undetected because of confounding clinical signs of SVV. Research with the baculovirus expression vector system (BEVS) has produced virus-like particles (VLP) that elicited neutralizing antibodies and protected against challenge for many Picornaviridae viruses. Building on this previous research, attempts of SVV VLP assembly for a vaccine candidate were performed using the BEVS.

All baculovirus constructs were designed to encode the ...


Identification And Characterization Of A Novel Structural Protein Of Porcine Reproductive And Respiratory Syndrome Virus, The Replicase Nonstructural Protein 2, Matthew Allan Kappes Jan 2014

Identification And Characterization Of A Novel Structural Protein Of Porcine Reproductive And Respiratory Syndrome Virus, The Replicase Nonstructural Protein 2, Matthew Allan Kappes

Graduate Theses and Dissertations

Porcine reproductive and respiratory syndrome virus (PRRSV) is a rapidly mutating pathogen eliciting respiratory and reproductive disease of high economic consequence. The PRRSV non-structural protein 2 (nsp2) is a large multifunctional protein encoded by the most genetically diverse region of the genome - the selective pressure potentiating mutation within this region is unknown. Here we report the identification of a unique function of nsp2 as a structural component of the PRRSV virion; the first PRRSV structural protein identified which is not expressed from a sub-genomic RNA or regulated via the discontinuous transcription pathway. Through the use of a set of custom ...


Development Of An In Vitro Model And Fluorescent Protein Expression System For The Study Of Highly Abortigenic Campylobacter Jejuni, Moria Ann Borys Jan 2014

Development Of An In Vitro Model And Fluorescent Protein Expression System For The Study Of Highly Abortigenic Campylobacter Jejuni, Moria Ann Borys

Graduate Theses and Dissertations

Campylobacter spp. are a leading cause of sheep abortions worldwide; Campylobacter fetus ssp. fetus has historically been the major culprit. Increasingly, however, C. jejuni has been isolated from cases of sheep abortion, and it has now replaced C. fetus ssp. fetus as the predominant cause of Campylobacter-related ovine abortion in the United States. Emergence of a single tetracycline resistant clone (clone SA) has been implicated as the primary reason for this shift. Virulence factors for clone SA have still not been completely elucidated, and it is not known how this bacterium reaches the uterine and placental tissue from the ...


Gene Mapping Of Monogenic Disorders And Complex Diseases Via Genome Wide Association Studies, Xia Zhao Jan 2012

Gene Mapping Of Monogenic Disorders And Complex Diseases Via Genome Wide Association Studies, Xia Zhao

Graduate Theses and Dissertations

Inherited rickets, lower motor neuron disease and chondrodysplasia were recently reported genetic disorders that had been identified on sheep farms in New Zealand. Animals with rickets showed softening and weakening bone, and were characterized by decreased growth rate, thoracic lordosis and angular limb deformities. Sheep diagnosed with lower motor neuron disease were characterized by poor muscle tone and progressive weakness from about one week of age, leading to severe tetraparesis, recumbency and muscle atrophy. Chondrodysplasia in Texel sheep was characterized by dwarfism and angular deformities of the forelimbs. These three disorders were determined likely simple autosomal recessive by backcross breeding ...