Open Access. Powered by Scholars. Published by Universities.®

Molecular Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 29 of 29

Full-Text Articles in Molecular Biology

River Bank Inducement Influence On A Shallow Groundwater Microbial Community And Its Effects On Aquifer Reactivity, Natalie June Gayner Dec 2018

River Bank Inducement Influence On A Shallow Groundwater Microbial Community And Its Effects On Aquifer Reactivity, Natalie June Gayner

Theses and Dissertations

Placing groundwater wells next to riverbanks to draw in surface water, known as riverbank inducement (RBI), is common and proposed as a promising and sustainable practice for municipal and public water production across the globe. However, these systems require further investigation to determine risks associated with river infiltration especially with rivers containing wastewater treatment plant (WWTP) effluent. Since microbes drive biogeochemical transformations in groundwater and largely affect water quality, it is important to understand how the microbial communities in drinking water wells are affected by river infiltration. This study investigated if, and to what extent, the microbial community in a ...


New Insights Into The Role Of Antimicrobials Of Xenorhabdus In Interspecies Competition, Kristin Jean Ciezki Aug 2017

New Insights Into The Role Of Antimicrobials Of Xenorhabdus In Interspecies Competition, Kristin Jean Ciezki

Theses and Dissertations

Xenorhabdus spp. are symbionts of entomopathogenic nematodes and pathogens of susceptible insects. The nematodes penetrate the insect midgut to enter the hemocoel where Xenorhabdus bacteria are released, transitioning to their pathogenic stage. During nematode invasion microbes from the insect gut translocate into the hemocoel. In addition, different species of nematodes carrying specific strains of Xenorhabdus can invade a single insect. Xenorhabdus spp thereby engage in competition with both related strains and nonrelated gut microbes. In complex media Xenorhabdus spp produce diverse antimicrobial compounds whose functions in biological systems remain poorly understood. R-type bacteriocins are contractile phage-tail-like structures that are bactericidal ...


Sex Differences In Differential Fear Conditioning During The Acquisition And Consolidation Of Learned Safety, David Sylvan Reis Aug 2017

Sex Differences In Differential Fear Conditioning During The Acquisition And Consolidation Of Learned Safety, David Sylvan Reis

Theses and Dissertations

The ability to distinguish between threatening and non-threatening situations requires careful regulation of behavioral and physiological responses to stress and fear. Deficits in fear regulation are maladaptive and can lead to the development of anxiety disorders such as PTSD. Women are nearly twice as likely to develop PTSD as are men and laboratory animal studies have shown facilitated fear acquisition, resistance to fear extinction, deficits in extinction retention and impaired discrimination between danger and safety cues in females. Taken together this suggests a propensity for reduced inhibitory control over fear responding in females. Here we investigate the mechanisms underlying fear ...


Microbial Communities And The Diverse Ecology Of Fecal Indicators At Lake Michigan Beaches, Danielle Cloutier May 2017

Microbial Communities And The Diverse Ecology Of Fecal Indicators At Lake Michigan Beaches, Danielle Cloutier

Theses and Dissertations

Fecal indicator bacteria (FIB) such as Escherichia coli (E. coli) and enterococci are used to assess microbiological water quality in recreational waters. The use of FIB follows the assumption that their presence correlates with that of fecal-associated pathogens in recreational waters. The beach ecosystem is complex however and multiple factors can influence the concentration of E. coli and enterococci in the beach environment. Microbial communities within beach sand play a key role in nutrient cycling and are important to the nearshore ecosystem function. E. coli and enterococci, two common indicators of fecal pollution, have been shown to persist in the ...


Towards Autonomous Microcystin Detection: Investigating Methods For Automation, Maureen Anne Schneider May 2017

Towards Autonomous Microcystin Detection: Investigating Methods For Automation, Maureen Anne Schneider

Theses and Dissertations

Due to increased anthropogenic activity, severe eutrophication is occurring in bodies of water around the world. Effects include decreased water quality, decreased value of surrounding land and recreational use (estimated loss in revenue of 0.67 and 3.96 U.S. billion dollars per year), and increased occurrence of toxin producing Harmful Algal Blooms (HABs). Microcystins are cyclic peptides made up of 7 amino acids and 800-1100 Daltons in size. They are one of the most predominantly produced of these toxins, and therefore was the focus of this study. Numerous structural variants of microcystin (referred to as congeners) exist, but ...


Molecular And Functional Analysis Of The Pixb Gene In Xenorhabdus Nematophila, John Lucas Dec 2016

Molecular And Functional Analysis Of The Pixb Gene In Xenorhabdus Nematophila, John Lucas

Theses and Dissertations

Xenorhabdus nematophila and the entomopathogenic nematode Steinernema carpocapsae form a mutualistic relationship facilitating the infection, death and consumption of an insect host. The infective juvenile (IJ) form of S. carpocapsae invades the insect host through natural openings and proceeds to the hemocoel where exposure to hemolymph stimulates the release of X. nematophila from the anterior vesicle. Excreted X. nematophila releases immunosuppressive compounds and insect toxins into the insect hemolymph that facilitates death of the host. As X. nematophila reaches high cell density it secretes exoenzymes that degrade insect tissues and produces antibiotics that reduce microbial competition. S. carpocapsae utilizes the ...


Characterization And Use Of Folate Receptor Isoforms For Targeting Of Epithelial And Myeloid Cells, Sreya Biswas Dec 2016

Characterization And Use Of Folate Receptor Isoforms For Targeting Of Epithelial And Myeloid Cells, Sreya Biswas

Theses and Dissertations

ABSTRACT

CHARACTERIZATION AND USE OF FOLATE RECEPTOR ISOFORMS FOR TARGETING OF EPITHELIAL AND MYELOID CELLS

by

Sreya Biswas

The University of Wisconsin-Milwaukee, 2016

Under the Supervision of Professor Douglas A. Steeber

Folate receptor (FR) is a GPI-anchored glycoprotein with high binding affinity for folic acid. FR has two membrane-associated isoforms, α and β, that are overexpressed on epithelial and myeloid tumors, respectively. Normal cells may also exhibit FR expression at very low levels but interestingly, FR-α on normal cells is restricted to the apical surface i.e., away from the blood stream. This differential expression and orientation of the FR-α ...


Lateral Replacement Of The Lux Operon In A Vibrio Isolated From The Intestine Of A Coral Reef Fish, Melissa Lee Whyte Aug 2016

Lateral Replacement Of The Lux Operon In A Vibrio Isolated From The Intestine Of A Coral Reef Fish, Melissa Lee Whyte

Theses and Dissertations

In a screening of bioluminescent bacteria isolated from the intestines of coral reef fish, two strains (designated D6 and M1) were identified that have a luxA gene sequence significantly different from those of other Vibrio species. Phylogenetic analysis of several housekeeping genes, as well as toxR, shows that D6 and M1 branch within a bioluminescent clade (designated the “D1 group,” isolated at the same time and place as D6 and M1) that is a close sister group to Vibrio harveyi. However, whereas the luxA genes of the D1 group are >98% identical to V. harveyi luxA, the luxA genes of ...


Discovery And Characterization Of A New Group Of Is10 Insertion Sequences, Rachel Marie Kinzelman Aug 2016

Discovery And Characterization Of A New Group Of Is10 Insertion Sequences, Rachel Marie Kinzelman

Theses and Dissertations

Insertion sequences (ISs) are small mobile genetic elements that can have significant impact on the genotype and phenotype of a host organism. Previous work in this laboratory revealed an insertion sequence that disrupted the luxA gene in Vibrio harveyi strain BCB451, knocking out light production. Phylogenetic analysis of this insertion sequence, dubbed IS451, reveals that it is in the IS10 family, but represents a novel variant that is only 79% identical to other known IS10 sequences. Twelve copies of IS451 were isolated from a genomic library and sequenced, and were found to be essentially identical, but located in dispersed chromosomal ...


Protective Effects Of Pigmentation In Pseudomonas Aeruginosa: Insights On Pyomelanin Production And Inhibition By Ntbc, Laura Marie Ketelboeter May 2016

Protective Effects Of Pigmentation In Pseudomonas Aeruginosa: Insights On Pyomelanin Production And Inhibition By Ntbc, Laura Marie Ketelboeter

Theses and Dissertations

Pyomelanin is an extracellular, reddish-brown pigment produced by several environmental and pathogenic bacteria and fungi. It is derived from the phenylalanine/tyrosine catabolism pathway and is produced when homogentisate (HGA) is secreted from the cell, auto-oxidized, and self-polymerized. Point mutations or chromosomal deletions in hmgA, which encodes homogentisate 1,2-dioxygenase, result in the accumulation of HGA and subsequent pyomelanin production. My work showed that the pyomelanogenic Pseudomonas aeruginosa clinical isolate PA1111 had a chromosomal deletion of hmgA, while a second pyomelanogenic clinical isolate, DKN343, had a loss of function mutation in HmgA. The 4-hydroxyphenylpyruvate dioxygenase (Hpd) inhibiting compound 2-(2-nitro-4-trifluoromethylbenzoyl ...


Role Of Protein Phosphatase Reg2-Glc7 In The Regulation Of The Yeast Stress Response Kinase, Snf1, Marcin Maziarz May 2016

Role Of Protein Phosphatase Reg2-Glc7 In The Regulation Of The Yeast Stress Response Kinase, Snf1, Marcin Maziarz

Theses and Dissertations

Kinases of the AMP-activated protein kinase (AMPK) family are conserved in eukaryotes and play central roles in responses to reduced energy availability. AMPK, nicknamed the “fuel gauge” of the cell, monitors cellular energy status via the ratio of AMP to ATP nucleotides. AMPK restores energy homeostasis by reducing energy “spending” and increasing energy “income”. Correspondingly, defects in AMPK signaling have been implicated in diseases including type II diabetes, obesity, and cancer.

In yeast, the AMPK homolog is Snf1 protein kinase. Glucose is the preferred carbon/energy source of yeast, and thus limitation for glucose similarly activates Snf1. Snf1 activation requires ...


Transport Mechanisms For Human Fecal Indicator Bacteria In An Urban Stormwater Basin In Southeastern Wisconsin, Chelsea M. Corson Dec 2015

Transport Mechanisms For Human Fecal Indicator Bacteria In An Urban Stormwater Basin In Southeastern Wisconsin, Chelsea M. Corson

Theses and Dissertations

Discharge of stormwater runoff to receiving waters is a known source of human pathogens; however the primary mechanisms by which these pathogens enter the stormwater system have yet to be quantified. This study builds upon and utilizes prior research findings in an attempt to explain the influence of the age of the pipes within stormwater and sanitary conveyance systems, rainfall and hydrogeological characteristics, and select infrastructure variables that contribute to the observed contamination of an urban stormwater basin in Southeastern Wisconsin.

Over the course of approximately two years from 2012 to 2014, a total of 260 samples from 22 stormwater ...


Role Of Non-Muscle Myosin Ii And Calcium In Zebrafish Midbrain-Hindbrain Boundary Morphogenesis, Srishti Upasana Sahu May 2015

Role Of Non-Muscle Myosin Ii And Calcium In Zebrafish Midbrain-Hindbrain Boundary Morphogenesis, Srishti Upasana Sahu

Theses and Dissertations

Elucidating the molecular mechanisms that play a role in cellular morphogenesis is critical to our understanding of brain development and function. The midbrain-hindbrain boundary (MHB) is one of the first folds in the vertebrate embryonic brain and is highly conserved across species. We used the zebrafish MHB as a model for determining the molecular mechanisms that regulate these cell shape changes. Cellular morphogenesis is tightly regulated by signaling pathways that rearrange the cytoskeleton and produce mechanical forces that enable changes in cell and tissue morphology. The generation of force within a cell often depends on motor proteins, particularly non-muscle myosins ...


Molecular Identification Of Mycobacterium Tuberculosis In The Milwaukee County Institution Grounds Cemetery, Helen Marie Werner May 2015

Molecular Identification Of Mycobacterium Tuberculosis In The Milwaukee County Institution Grounds Cemetery, Helen Marie Werner

Theses and Dissertations

The possibility of identifying Mycobacterium tuberculosis in skeletal remains has been a debated topic for many years. This study utilizes the remains from the 1991 and 1992 excavations of the Milwaukee County Institution Grounds Cemetery, a collection of human skeletons ranging from 1882 to 1925, of various ages and sexes, to address that possibility. To test the utility of previously used methods of osteological identification of tuberculosis, the collection has been analyzed for the IS6110 repetitive element marker using molecular biological techniques, such as Polymerase Chain Reaction (PCR). Eighty-six skeletons from the collection have been analyzed, with nine of them ...


Novel Protein Secretion And Chitin Utilization Machinery Of Flavobacterium Johnsoniae, Sampada Suresh Kharade Dec 2014

Novel Protein Secretion And Chitin Utilization Machinery Of Flavobacterium Johnsoniae, Sampada Suresh Kharade

Theses and Dissertations

Flavobacterium johnsoniae, a member of phylum Bacteroidetes, is a gliding bacterium that digests insoluble chitin. A novel protein secretion system, the Type IX secretion system (T9SS), secretes the motility adhesins SprB and RemA and is also required for chitin utilization. In order to understand F. johnsoniae chitin utilization and the role of the T9SS, Fjoh_4555 (chiA) was targeted for analysis. Disruption of chiA resulted in cells that failed to digest chitin and complementation restored this ability. Antisera raised against ChiA were used to characterize its secretion. ChiA was secreted in soluble form by wild-type cells but remained cell-associated in T9SS ...


Interaction Of Rhizobium Sp. Strain Irbg74 With A Legume (Sesbania Cannabina) And A Cereal (Oryza Sativa), Shubhajit Mitra Dec 2014

Interaction Of Rhizobium Sp. Strain Irbg74 With A Legume (Sesbania Cannabina) And A Cereal (Oryza Sativa), Shubhajit Mitra

Theses and Dissertations

Rhizobium sp. IRBG74 (IRBG74) develops a classical nitrogen-fixing symbiosis with the legume Sesbania cannabina and also promotes the growth of rice (Oryza sativa), but not much is known about the rhizobial determinants important for these interactions. We hypothesize that Rhizobium sp. IRBG74 utilizes similar mechanisms to endophytically colonize both legume and cereal hosts. In this study, we analyzed the colonization of rice and S. cannabina using a strain of IRBG74 marked with β-glucuronidase (GUS) and Green Fluorescent Protein (GFP). IRBG74 infected both of the host plants through crack entry under submerged conditions, but showed root hair mediated infection under aerobic ...


Gene Regulatory Pathways Driving Central Nervous System Regeneration In Zebrafish, Ishwariya Venkatesh Dec 2014

Gene Regulatory Pathways Driving Central Nervous System Regeneration In Zebrafish, Ishwariya Venkatesh

Theses and Dissertations

Damage to the central nervous system (CNS) circuitry of adult mammals results in permanent disability. In contrast, the ability to regenerate damaged CNS nerves and achieve functional recovery occurs naturally in fish. The ability of fish to successfully regrow damaged CNS nerves is in part a consequence of their ability to re-express key neuronal growth-associated genes/proteins in response to CNS injury. On such protein is Growth-Associated Protein-43 (Gap43), a protein which is highly enriched in axonal growth cones during CNS development and regeneration. Experiments conducted in mammals have demonstrated that ectopic expression of GAP-43 improves axonal re-growth after injury ...


Development Of Peripheral Innervation In The Frog Xenopus Laevis, Mitali A. Gandhi Aug 2014

Development Of Peripheral Innervation In The Frog Xenopus Laevis, Mitali A. Gandhi

Theses and Dissertations

The skin in Xenopus laevis is innervated by two different sets of mechanosensory neurons at different times during development. Rohon Beard (RB) neurons start differentiating during gastrulation, innervate the embryonic skin and mediate sensory function during hatching. Dorsal Root Ganglion (DRG) neurons start differentiating after neural crest migration, innervate adult epidermal targets and mediate mechanosensory function during larval and adult stages and eventually replace RB neurons. The change in sensory neurons occurs during the transformation of skin, sensory structures, and behavior from their embryonic to their larval forms. We hypothesized that developmental changes in either the sensory end organs or ...


The Role Of Antimicrobial Compounds In The Life Cycle Of The Symbiotic Bacterium, Xenorhabdus Nematophila, Swati Singh May 2014

The Role Of Antimicrobial Compounds In The Life Cycle Of The Symbiotic Bacterium, Xenorhabdus Nematophila, Swati Singh

Theses and Dissertations

The bacterium Xenorhabdus nematophila maintains a mutualistic relationship with the entomopathogenic nematode Steinernema carpocapsae and is also pathogenic towards insect larvae. X. nematophila possesses a large number of gene clusters potentially involved in antimicrobial production. Several antibiotics, including xenocoumacin (Xcn) produced at high levels in broth cultures, have been characterized. In this study I established that during nematode invasion of the insect body cavity (hemocoel) gut microbiota enter the hemocoel representing potential competitors for X. nematophila. As infection progressed some transient species, such as Staphylococcus saprophyticus disappeared early in infection, while other persistent species such as Enterococcus faecalis proliferated. S ...


Enzyme Activity, Maturation And Regulation Of Anaerobic Reductases In Shewanella Oneidensis Mr-1, Kenneth L. Brockman May 2014

Enzyme Activity, Maturation And Regulation Of Anaerobic Reductases In Shewanella Oneidensis Mr-1, Kenneth L. Brockman

Theses and Dissertations

Shewanella oneidensis MR-1 is a metal-reducing bacterium capable of using a wide range of terminal electron acceptors. These include oxygen, metal oxides and organic compounds such as dimethyl sulfoxide (DMSO) and fumarate. In addition, several nitrogen and sulfur based compounds can be used as terminal electron acceptors, including sulfite, for which the terminal reductase was recently identified as an octaheme c-type cytochrome that contains an atypical heme binding site. In this study, several additional components involved in sulfite reduction were identified. These include SirCD that form a membrane-bound electron-transferring complex with SirA, SirBI that appear to be involved in protein ...


Small Rna, Cyclic-Di-Gmp And Phenolic Compounds Regulate The Type Iii Secretion System In Bacterial Phytopathogens, Devanshi Khokhani May 2014

Small Rna, Cyclic-Di-Gmp And Phenolic Compounds Regulate The Type Iii Secretion System In Bacterial Phytopathogens, Devanshi Khokhani

Theses and Dissertations

Type III Secretion System (T3SS) is an essential virulence factor in many Gram-negative bacterial pathogens. Expression of T3SS consumes large amount of energy. Hence it is tightly regulated by bacteria through several mechanisms. In this work we screened a library of phenolic compounds and found several compounds that

dramatically downregulate T3SS in Erwinia amylovora 273. Additionally, the role of small RNA (sRNA) chaperone, Hfq, and a secondary messenger, cyclic-di-GMP in T3SS regulation in Dickeya dadantii 3937 was also examined. Chapter 1 provides a brief overview of the history and virulence mechanisms of two phytopathogens - Erwinia amylovora 273 and Dickeya dadantii ...


The Role Of Gap-43 Phosphorylation In Axon Behavior In The Developing Zebrafish Visual System, Jennifer Forecki Dec 2013

The Role Of Gap-43 Phosphorylation In Axon Behavior In The Developing Zebrafish Visual System, Jennifer Forecki

Theses and Dissertations

Developing neurons extend processes to specific targets and establish connections that are essential for future function of the nervous system. One of these processes, the axon, has a motile tip called a growth cone that rearranges its membrane-associated actin cytoskeleton to turn toward or away from environmental guidance cues. Growth associated protein 43 (GAP-43) is one of the most abundant proteins associated with axonal growth cone membranes and is known to modulate the formation and stability of the actin cytoskeleton during axon guidance. Protein kinase C (PKC)-mediated phosphorylation of GAP-43 on serine 42 regulates its interactions with actin. Phosphorylated ...


Transcending Microbial Source Tracking Techniques Across Geographic Borders: An Examination Of Human And Animal Microbiomes And The Integration Of Molecular Approaches In Pathogen Surveillance In Brazil And The United States, Amber Mae Koskey Dec 2013

Transcending Microbial Source Tracking Techniques Across Geographic Borders: An Examination Of Human And Animal Microbiomes And The Integration Of Molecular Approaches In Pathogen Surveillance In Brazil And The United States, Amber Mae Koskey

Theses and Dissertations

Waterborne illnesses, attributed to the ingestion or contact with contaminated water, present a significant global health concern. Surface water sources can be impacted by wide array of pollution inputs, but fecal pollution generates the most significant and acute threat to human health. Therefore, the detection of fecal bacteria in surface water sources remains an important public health objective. Current surface water monitoring employs the use of fecal indicator bacteria (FIB) including E. coli and enterococci as proxies for pathogenic organisms carried in fecal pollution. These traditional indicators, detected by culture-based microbiological methods, do not discriminate fecal sources from another. New ...


Evaluating The Influence Of Environmental Factors On The Rate Of Extra-Pair Matings In Tropical And Temperate Populations Of The House Wren (Troglodytes Aedon), Kaitlin Claire Mckenney Dec 2013

Evaluating The Influence Of Environmental Factors On The Rate Of Extra-Pair Matings In Tropical And Temperate Populations Of The House Wren (Troglodytes Aedon), Kaitlin Claire Mckenney

Theses and Dissertations

Considerable variation exists in the rate of extra-pair matings (EPMs) in birds. Environmental variability likely influences EPM rates within species, but the effects of local environmental factors on EPM rates are largely unpredictable. To determine whether broad-scale environmental factors might be better predictors of EPM rates within species, we quantified levels of extra-pair paternity in the house wren (Troglodytes aedon) in four populations spanning a range of latitude, elevation, and primary productivity (measured by actual evapotranspiration rates). Our results indicated an intermediate and variable level of EPM among populations (6 -31% extra-pair young) that was not significantly affected by 3 ...


Quorum Sensing In Vibrios And Cross-Species Activation Of Bioluminescence Lux Genes By Vibrio Harveyi Luxr In An Arabinose-Inducible Escherichia Coli Expression System, Anne Marie Wannamaker May 2013

Quorum Sensing In Vibrios And Cross-Species Activation Of Bioluminescence Lux Genes By Vibrio Harveyi Luxr In An Arabinose-Inducible Escherichia Coli Expression System, Anne Marie Wannamaker

Theses and Dissertations

Bacterial bioluminescence is observed in over twenty known species, primarily in the family Vibrionaceae. However, only Vibrio fischeri and Vibrio harveyi bioluminescence expression mechanisms are well studied. In V. harveyi, expression of the lux operon is activated by the transcription factor LuxR (LuxRVH), resulting in bioluminescence. Homologs of LuxRVH in other Vibrio species have been shown to regulate transcription of a variety of genes. Three parallel quorum sensing pathways co-regulate the expression of LuxRVH. The first objective was to assess possible quorum sensing regulation of lux operon expression in V. cholerae, V. chagasii, V. orientalis, and V ...


Prolonged Glucose Deprivation Sensitizes Snf1 To Negative Regulation By Pka To Delay Entry Into Quiescence, Leah Bernadette Doughty May 2013

Prolonged Glucose Deprivation Sensitizes Snf1 To Negative Regulation By Pka To Delay Entry Into Quiescence, Leah Bernadette Doughty

Theses and Dissertations

AMPK, the fuel gauge of the cell, and its upstream kinase, LKB1, have been implicated in cancer prevention and stress response associated with energy exhaustion. In the yeast Saccharomyces cerevisiae, Snf1 is the ortholog of mammalian AMPK. In S. cerevisiae, Snf1 is activated by phosphorylation of its T–loop at Thr210, primarily by its upstream kinase Sak1, in absence of the preferred carbon source, glucose, or during some other stress responses. Cyclic AMP–dependent protein kinase A, PKA, is involved in nutrient signaling largely antagonistically to Snf1. Using yeast strains of the Sigma 1278b genetic background, which have a high ...


Cell Surface Adhesins, Exopolysaccharides And The Por (Type Ix) Secretion System Of Flavobacterium Johnsoniae, Abhishek Shrivastava May 2013

Cell Surface Adhesins, Exopolysaccharides And The Por (Type Ix) Secretion System Of Flavobacterium Johnsoniae, Abhishek Shrivastava

Theses and Dissertations

Flavobacterium johnsoniae, a member of the phylum Bacteroidetes, crawls rapidly over surfaces. Cell movement is thought to result from the action of the gliding motor, composed of Gld proteins, on the cell-surface adhesin SprB. Cells lacking SprB are partially defective in motility. Transposon mutagenesis of an sprB mutant resulted in the identification of remA, which encodes a motility adhesin that is partially redundant with SprB. Cells lacking SprB and RemA had more severe motility defects than did cells lacking just SprB. RemA moves on the cell-surface with a speed of 1 to 2 micrometer per sec, similar to SprB. RemA ...


Gene Expression Response In Early Developmental Stages Of Rainbow Trout Exposed To Ecologically Relevant Concentrations Of Malathion, Susan Miller Dec 2012

Gene Expression Response In Early Developmental Stages Of Rainbow Trout Exposed To Ecologically Relevant Concentrations Of Malathion, Susan Miller

Theses and Dissertations

Understanding the early life stage toxic effects of environmental organophosphate exposure on organism health is crucial to identifying biomarkers that can be used for preventative care. Malathion, a potent organophosphate, is one of the most widely used organophosphates in agriculture and pest eradication. Due to its widespread use, pesticide runoff into area bodies of water poses a great threat to aquatic life and human inhabitants. Acute exposure to high concentrations of malathion causes neurological abnormalities and can result in respiratory failure, muscle spasms, and mental confusion in humans.

In the present study, the effects of malathion are observed following acute ...


Assessment Of Dim Mutants Of The Vibrio Harveyi Species Isolated From The Gulf Of Mexico, Elizabeth Bolton Aug 2012

Assessment Of Dim Mutants Of The Vibrio Harveyi Species Isolated From The Gulf Of Mexico, Elizabeth Bolton

Theses and Dissertations

Isolating environmental dim and dark mutants of V. harveyi gives insight into the positive selection and maintenance of lux genes in a natural setting. Mutants were isolated from Boca Ciega Bay, FL., on the basis of the presence of lux genes without visible luminescence when plated on SWC agar. Of 600 isolates, four mutants were found to glow minimally on X-ray film. Of these four, luminescence was rescued in three isolates to a varying degree by addition of aldehyde, a component of the bacterial luminescence reaction. These four lux operons were sequenced, and two were shown to have a frameshift ...