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Articles 1 - 10 of 10
Full-Text Articles in Molecular Biology
Resistance Screening And Association Mapping For Resistance To The Downy Mildew Pathogen Of Spinach, Dotun Olaoye
Resistance Screening And Association Mapping For Resistance To The Downy Mildew Pathogen Of Spinach, Dotun Olaoye
Graduate Theses and Dissertations
Spinach is an important cool leafy vegetable cultivated around the world, with large scale production in California and Arizona in the U.S. Spinach is a highly nutritious vegetable beneficial in the human diet. Spinach is affected by a number of biotic stressors. Downy mildew, caused by the oomycete pathogen Peronospora effusa, is a major threat to spinach as it affects the leaf quality and impacts the economic value of spinach. Several efforts have led to the development of resistant genotypes/cultivars to this pathogen. However, few studies have examined the genetics of resistance to the downy mildew pathogen in detail. This …
Taxonomic And Genetic Diversity, And Pathogenicity Of Diaporthe Species Associated With Soybean, Fakhir Eraheem Hameed Al Shuwaili
Taxonomic And Genetic Diversity, And Pathogenicity Of Diaporthe Species Associated With Soybean, Fakhir Eraheem Hameed Al Shuwaili
Graduate Theses and Dissertations
Diaporthe species (anamorph: Phomopsis) are associated with a wide range of plant hosts as plant pathogens, asymptomatic endophytes, and saprobes. One of these hosts is soybean, which is one of the most important crops in U.S. agriculture. Several Diaporthe species cause important diseases on soybean in the U.S., and specifically in Arkansas. The taxonomy, genetic diversity, and pathogenicity of Diaporthe species associated with asymptomatic infection of soybean are rarely studied with accurate molecular tools. Therefore, this dissertation aimed to assess the diversity and boundaries of Diaporthe associated with soybean in Arkansas. Furthermore, pathogenicity and alternative lifestyles were assessed among Diaporthe …
Dissecting The Cellular Control Of Septin Organization In A Global Cereal Killer, Nawaraj Dulal
Dissecting The Cellular Control Of Septin Organization In A Global Cereal Killer, Nawaraj Dulal
Graduate Theses and Dissertations
Rice blast disease, caused by the filamentous fungus Magnaporthe oryzae, destroys sufficient rice each year to feed 60 million people, and is a serious threat to global food security. A wheat-adapted lineage of M. oryzae now poses threat to global wheat production. Rice blast disease is currently controlled using limited fungicides, and the emergence of fungicide resistance within M. oryzae populations is a growing concern. There is a pressing need to identify new classes of fungicides to control the disease, which requires better understanding of the basic biology of the pathogen. To establish disease, M. oryzae forms a specialized dome …
Epidemiological Studies Of Soybean Vein Necrosis Virus And Potential Resistance Mechanisms To Its Vector Neohydatothrips Variabilis (Beach), Jing Zhou
Graduate Theses and Dissertations
Soybean (Glycine max (L.) Merrill) is one the most important crops in global agriculture with annual production of over 260 million metric tons. As the dependence of a growing global population to soybean has increased, so does the importance of soybean diseases and pests. Over 200 pathogens attack soybean; among them, viruses pose a major threat to the soybean industries accounting for approximately 10% of the annual yield reduction caused by diseases in the past two decades. Soybean vein necrosis virus (SVNV) is a relatively newly discovered virus causing the homonymous disease. The widespread occurrence of the disease in major …
Blackberry Virosome: A Micro And Macro Approach, Archana Khadgi
Blackberry Virosome: A Micro And Macro Approach, Archana Khadgi
Graduate Theses and Dissertations
Viruses pose a major concern for blackberry production around the world with more than 40 species known to infect the crop. Virus complexes have been identified recently as the major cause of plant decline with blackberry yellow vein disease (BYVD) being the most important disease of the crop in the Southern United States. The objective of this research was to study the blackberry virosome in both the macro and micro scale. The large scale approach involves identification of the major viruses known to be associated with BYVD in the Southern United States as well as the identification of other viruses …
Understanding The Causal Agent Of Rose Rosette Disease, Patrick Louis Di Bello
Understanding The Causal Agent Of Rose Rosette Disease, Patrick Louis Di Bello
Graduate Theses and Dissertations
A number viruses are known to infect roses, ranging from those in the genera Nepovirus, and Ilarvirus, which have been reported since the inception of rose virology, to recently discovered viruses in the genera Carmovirus, Closterovirus, Emaravirus, Luteovirus, Rosadnavirus, and Potyvirus. Of the viral diseases in rose, arguably the most damaging is Rose rosette (RRD), which is associated with the Emaravirus, Rose rosette virus (RRV). The objective of this thesis is to fill in the gaps in knowledge on the epidemiological aspects of RRD and RRV. There has been significant progress in the epidemiology of the RRD agent prior to …
Biology And Control Of Rice False Smut Caused By Ustilaginoidea Virens (Teleomorph Villosiclava Virens), Andrew Clayton Jecmen
Biology And Control Of Rice False Smut Caused By Ustilaginoidea Virens (Teleomorph Villosiclava Virens), Andrew Clayton Jecmen
Graduate Theses and Dissertations
Rice false smut (FS), a disease caused by Ustilaginoidea virens (Cke.) Takahashi (1896), was first reported in northeastern Arkansas counties in 1997. The first objective of this research was to establish a collection of U. virens isolates from geographically diverse regions of Arkansas. Three U. virens isolates and chlamydospores from `Templeton' and `Clearfield-151' rice cultivars were used to determine the effects of temperature and pH on mycelial growth and germination. A nested-PCR protocol and histological methods were used to determine if U. virens infects and colonizes rice seedlings and spikelets on panicles. The sensitivity of three U. virens isolates was …
Molecular And Biochemical Mechanisms Of Pathogenesis In The Maize Foliar Pathogen Cercospora Zeae-Maydis, Winfred-Peck Dorleku
Molecular And Biochemical Mechanisms Of Pathogenesis In The Maize Foliar Pathogen Cercospora Zeae-Maydis, Winfred-Peck Dorleku
Graduate Theses and Dissertations
GLS is a serious foliar disease of maize, a major staple crop grown commercially in the USA for both human and animal feed production, and increasingly, for ethanol production. The disease is caused by two Cercospora species, C. zeae-maydis and C. zeina, both of which infect maize in the USA and in other parts of the world, with yield losses potentially greater than 50%, depending on local conditions. In culture, C. zeae-maydis produces a phytotoxic, host non-specific perylenequinone, cercosporin, and abscisic acid (ABA), for which there is no known pathological or physiological function in the fungus. Experimental evidence indicates …
Characterization And Epidemiology Of Soybean Vein Necrosis Associated Virus, Jing Zhou
Characterization And Epidemiology Of Soybean Vein Necrosis Associated Virus, Jing Zhou
Graduate Theses and Dissertations
Soybean vein necrosis disease (SVND) is widespread in major soybean-producing areas in the U.S. The typical disease symptoms exhibit as vein clearing along the main vein, which turn into chlorosis or necrosis as season progresses. Double-stranded RNA isolation and shot gun cloning of symptomatic tissues revealed the presence of a new tospovirus, provisionally named as Soybean vein necrosis associated virus (SVNaV). The presence of the virus has been confirmed in 12 states: Arkansas, Illinois, Missouri, Kansas, Tennessee, Kentucky, Mississippi, Maryland, Delaware, Virginia and New York. Symptomatic samples collected from eight states (AR, IL, MO, MS, KS, TN, MD and DE), …
Gene Expression And Physiological Analyses To Study Metabolic Shifts In Medicago Truncatula Subjected To Biotic Stresses, Michelle Ryan Martin
Gene Expression And Physiological Analyses To Study Metabolic Shifts In Medicago Truncatula Subjected To Biotic Stresses, Michelle Ryan Martin
Graduate Theses and Dissertations
Plants are frequently under attack from pests and deploy various defense mechanisms to fight off predators. Many plant defenses are induced following herbivory or pathogen infection. An investment in defense could potentially lead to a diversion of metabolic resources away from primary metabolism, including reduction in plant commitment to photosynthesis. In this thesis, changes in expression levels of representative genes for primary and secondary metabolism were studied using real-time polymerase chain reaction (PCR) in Medicago truncatula Gaertner subjected to beet armyworm (Spodoptera exigua H¨¹bner) damage or Phoma medicaginis Malbr. & Roum. infection. Photosynthetic yield was also measured using chlorophyll fluorescence …