Molecular Biology Commons^™

Structural And Biochemical Investigation Of The Molecular Mechanisms Of Dna Response And Repair In Humans And Escherichia Coli., Steven Michael Shell

Electronic Theses and Dissertations

The genomes of all living cells are under constant attack from both endogenous and exogenous agents that damage DNA. In order to maintain genetic integrity a variety of response pathways have evolved to recognize and eliminate DNA damage. Replication protein A (RPA), the eukaryotic single-stranded DNA (ssDNA) binding protein, is a required factor for all major DNA metabolisms. Although much work has been done to elucidate the nature of the interaction between RPA and ssDNA currently there is no structural information on how the full-length protein binds to ssDNA. This study presents a novel examination of the full nucleoprotein complex …