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Theses/Dissertations

Physical Sciences and Mathematics

Analytic

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Articles 1 - 3 of 3

Full-Text Articles in Molecular Biology

Optimization Of Separation And Detection Schemes For Dna With Pulsed Field Slab Gel And Capillary Electrophoresis , David Arthur Mcgregor Jan 1993

Optimization Of Separation And Detection Schemes For Dna With Pulsed Field Slab Gel And Capillary Electrophoresis , David Arthur Mcgregor

Retrospective Theses and Dissertations

The purpose of the Human Genome Project is outlined followed by a discussion of electrophoresis in both slab gels and capillaries and its application to deoxyribonucleic acid (DNA). Techniques used to modify electroosmotic flow in capillaries are also addressed. Several separation and detection schemes for DNA via gel and capillary electrophoresis are described. Emphasis is placed on the elucidation of DNA fragment size in real time as well as shortening separation times to approximate real time monitoring;First, the migration of DNA fragment bands through a slab gel can be monitored by UV absorption at 254 nm and imaged by ...


Fluorescence Detection For Gel And Capillary Electrophoresis , Barry L. Hogan Jan 1992

Fluorescence Detection For Gel And Capillary Electrophoresis , Barry L. Hogan

Retrospective Theses and Dissertations

Several separation and detection schemes for gel and capillary electrophoresis are described. Emphasis is placed upon direct and indirect fluorescence detection systems as utilized for the determination of proteins, peptides, and intracellular analytes;First, an indirect fluorescence detection system for the separation of proteins via gel electrophoresis is described. Quantities as low as 50 nanograms of bovine serum albumin and soybean trypsin inhibitor are separated and detected visually without the need for staining of the analytes. This is very similar to levels of protein commonly separated with gel electrophoresis;A separation and detection system utilizing indirect fluorescence detection with capillary ...


Novel Detection Schemes And Automated Image Analysis Algorithms For Planar Chromatography And Gel Electrophoresis , Lance Bryant Koutny Jan 1992

Novel Detection Schemes And Automated Image Analysis Algorithms For Planar Chromatography And Gel Electrophoresis , Lance Bryant Koutny

Retrospective Theses and Dissertations

After a detailed introduction to Charge Coupled Devices (CCDs) and a discussion of personal computer capabilities, examples of their applications involving novel analytical techniques are presented;A new laser-based indirect fluorometric detection method for thin-layer chromatography is demonstrated with digitally controlled two-dimensional scanning and computer-aided image acquisition and vision. Utilizing this mode, universal detection is possible without derivatization, with a detection limit of 6 pg; 100 times lower than with human vision;A detection scheme for DNA in agarose slab gel electrophoresis based on ultraviolet absorption allows for convenient in situ monitoring of conventional gels via a CCD camera system ...