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Articles 1 - 4 of 4

Full-Text Articles in Molecular Biology

Theoretical Simulations And Ultrafast Pump-Probe Spectroscopy Experiments In Pigment-Protein Photosynthetic Complexes , Daniel Richard Buck Jan 1998

Theoretical Simulations And Ultrafast Pump-Probe Spectroscopy Experiments In Pigment-Protein Photosynthetic Complexes , Daniel Richard Buck

Retrospective Theses and Dissertations

Theoretical simulations and ultrafast pump-probe laser spectroscopy experiments were used to study photosynthetic pigment-protein complexes and antennae found in green sulfur bacteria such as Prosthecochloris aestuarii, Chloroflexus aurantiacus, and Chlorobium tepidum. The work focused on understanding structure-function relationships in energy transfer processes in these complexes through experiments and trying to model that data as we tested our theoretical assumptions with calculations;Theoretical exciton calculations on tubular pigment aggregates yield electronic absorption spectra that are superimpositions of linear J-aggregate spectra. The electronic spectroscopy of BChl c/d/e antennae in light harvesting chlorosomes from Chloroflexus aurantiacus differs considerably from J-aggregate spectra ...


The Poplar-Leaf Rust Pathosystem: Inheritance Of Resistance And Pathogenic Variability , Girma Melesse Tabor Jan 1998

The Poplar-Leaf Rust Pathosystem: Inheritance Of Resistance And Pathogenic Variability , Girma Melesse Tabor

Retrospective Theses and Dissertations

Among diseases that cause serious yield reduction on Populus deltoides is leaf rust caused by Melampsora medusae. The objectives of this project were to study the pathogenic variability of M. medusae, the genetic basis of inheritance of M. medusae resistance in a family of P. deltoides; and to identify molecular markers linked to a leaf-rust resistant locus. Three pathotypes of M. medusae were identified on the basis of their differential reaction to three Populus clones. Pathotypes D-93, F-93, and IL-48 were compatible with clone 1-488 (P. X euramericana). F-93 and IL48 were also compatible with clone 57-276 (P. deltoides X ...


An Integrated Multiple Capillary Array Electrophoresis System For High-Throughput Dna Sequencing , Xiandan Lu Jan 1998

An Integrated Multiple Capillary Array Electrophoresis System For High-Throughput Dna Sequencing , Xiandan Lu

Retrospective Theses and Dissertations

Capillary array electrophoresis system was chosen to perform DNA sequencing because of several advantages such as rapid heat dissipation, multiplexing capabilities, gel matrix filling simplicity, and the mature nature of the associated manufacturing technologies;There are two major concerns for the multiple capillary systems. One concern is inter-capillary cross-talk and the other concern is excitation and detection efficiency. Cross-talk is eliminated through proper optical coupling, good focusing and immersing capillary array into index matching fluid. A side-entry excitation scheme with orthogonal detection was established for large capillary array. Two 100 capillary array formats were used for DNA sequencing. One format ...


Comparative Evolution Of Molecular Markers: An Analysis Of Genetic Variation Within The Blue Marlin (Makaira Nigricans), Vincent Patrick Buonaccorsi Jan 1998

Comparative Evolution Of Molecular Markers: An Analysis Of Genetic Variation Within The Blue Marlin (Makaira Nigricans), Vincent Patrick Buonaccorsi

Dissertations, Theses, and Masters Projects

Blue marlin diversity was assessed at mtDNA, scnDNA, microsatellite DNA, and allozyme molecular markers. Hierarchical analysis of molecular variance (AMOVA) revealed that most genetic variation was maintained within populations, with a non-significant fraction attributable to variation among temporal replicates and between locations within oceans. In contrast, inter-ocean divergence was highly significant for a majority of loci within each marker class. Previous studies of mitochondrial DNA (mtDNA; n = 104) genetic variation within the blue marlin revealed two distinct clades of haplotypes, one of which was present only in the Atlantic (the 'Atlantic clade'), at a frequency of 40% &(F\sb{lcub ...