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Full-Text Articles in Molecular Biology

Molecular Marker Analysis Of Iron Efficiency In Soybean (Glycine Max (L.) Merr.) , Shun-Fu Lin Jan 1996

Molecular Marker Analysis Of Iron Efficiency In Soybean (Glycine Max (L.) Merr.) , Shun-Fu Lin

Retrospective Theses and Dissertations

The objectives of the studies were to map genes affecting iron deficiency chlorosis in soybean, to test the hypothesis that two genetic mechanisms control iron deficiency chlorosis in soybean, and to determine the effectiveness of nutrient solution evaluation for iron deficiency chlorosis in soybean. Chlorosis symptoms of one hundred and twenty F2:4 lines from a Pride B216 x A15 population, and 92 F2:4 lines from an Anoka x A7 population grown in a field of calcareous soil in 1993 and 1994 were evaluated by visual scores and spectrometric chlorophyll determinations. Each population also was evaluated separately with two ...


Integration Specificity Of The Retrovirus-Like Transposable Element Ty5 Of Saccharomyces , Sige Zou Jan 1996

Integration Specificity Of The Retrovirus-Like Transposable Element Ty5 Of Saccharomyces , Sige Zou

Retrospective Theses and Dissertations

The retrotransposons and retroviruses display a strong preference in selecting integration sites. The molecular mechanisms underlying this process, however, are largely unknown. I have characterized a new retrotransposon, called Ty5, in diverse strains of Saccharomyces cerevisiae and related species. A functional element, Ty5-6p, was identified from S. paradoxus. To investigate Ty5 target preference, the locations of 13 native insertions were determined in S. cerevisiae and S. paradoxus. Twelve were found near the telomeres and the mating locus HMR. To determine if this distribution is a consequence of targeted integration, a Ty5 transposition assay was developed in S. cerevisiae using Ty5-6p ...


Genetic Construction And Biochemical Analysis Of Thermostability Mutants Of Glucoamylase From Aspergillus Awamori , Yuxing Li Jan 1996

Genetic Construction And Biochemical Analysis Of Thermostability Mutants Of Glucoamylase From Aspergillus Awamori , Yuxing Li

Retrospective Theses and Dissertations

To study the molecular basis of Aspergillus awamori glucoamylase (GA) thermostability, eighteen mutants were constructed by site-directed mutagenesis and expressed in Saccharomyces cerevisiae based on thermostability theories. Four lysine residues, K61, K279, K352 and K404, were replaced with arginine, with all but K404 well exposed to the solvent and far away from the enzyme activity site. Mutations K61F/D65E and H254W/E326Q were made to fill a packing void around the inner set of six [alpha]-helices of GA and to displace water molecules inside the void. Five residues (A27, A393, A435, Ser436 and Ser460) were replaced with proline. Two ...


Characterization Of Membrane-Associated Nuclease Activity In Mycoplasma Pulmonis , Karalee Jean Jarvill-Taylor Jan 1996

Characterization Of Membrane-Associated Nuclease Activity In Mycoplasma Pulmonis , Karalee Jean Jarvill-Taylor

Retrospective Theses and Dissertations

Mycoplasmas are deficient in numerous biosynthetic pathways. Consequently, growth is dependent upon the acquisition of structural and macromolecular precursors from the environment. These precursors include phospholipids, fatty acids and nucleotide bases. DNases and RNases are postulated to be involved in the acquisition of nucleotides. The goals of this study were to determine if nuclease activities are universally associated with mycoplasmas, characterize the nucleases by subcellular distribution and apparent molecular weight, and to identify and analyze the nuclease gene(s) in Mycoplasma pulmonis;Nuclease activity was present in all mycoplasma species tested. The level of activity did not correlate with virulence ...