Molecular Biology Commons^™

Anti-Crispr Vs. Crispr: The Evolutionary Arms Race Between Microorganisms, Rachael M. St. Jacques

Masters Theses, 2010-2019

CRISPR arrays are a defense mechanism employed by bacteria against viral invaders. Cas proteins do the work in detecting, capturing, and integrating the viral DNA into the CRISPR array (Barrangou et al., 2007). Anti-CRISPR proteins are produced by phages, viruses that infect bacteria, to stop the bacterial host’s CRISPR-Cas complex from interrupting the phage life cycle (Bondy-Denomy, et al., 2015).

SEA-PHAGES is a course-based bacteriophage research network composed of 120 colleges and known at James Madison University as Viral Discovery. JMU uses the unsequenced Streptomyces griseus ATCC10137 as a host for bacteriophage discovery and propagation, and in this study we …

The Dna Dependent Protein Kinase Catalytic Subunit Maintains Genomic Stability, Brandon Lee Sklavanitis

Graduate Research Theses & Dissertations

GBM is the most common CNS derived malignancy diagnosed each year. The median survival time to date is between 14.6 to 16.7 months for patients suffering from the disease. Clinical trials and research has yet to yield a breakthrough treatment. The invasive nature of GBM to surrounding tissue typically poses a challenge for complete surgical resection of the tumor, and radiotherapy remains a predominant treatment modality. The NHEJ pathway is main repair pathway responsible for repairing lethal radiation damage. As a result, this study used the CRISPR-Cas9 system to genetically engineer two GBM cell lines lacking expression of an essential …