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Full-Text Articles in Molecular Biology

33 - Identification Of Proteins That Regulate Crispr Dna Uptake Of Pyrococcus Furiosus, Landon Clark Nov 2018

33 - Identification Of Proteins That Regulate Crispr Dna Uptake Of Pyrococcus Furiosus, Landon Clark

Georgia Undergraduate Research Conference (GURC)

The CRISPR-Cas (Clustered Regularly Interspace Short Palindromic Repeats-CRISPR associated) system is a prokaryotic, adaptive immune system used by bacterial and archaeal organism to fight infections by viruses and other harmful invasive DNAs. These prokaryotic CRISPR-Cas immune systems have been exploited as powerful genome editing tools that work many different organisms and cells including humans. The newly developed CRISPR-based technologies are transforming medicine and science and have been used in research applications for developing cures for certain cancers, HIV, hemophilia, etc. The function of the CRISPR-Cas systems follow three basis steps: (1) adaptation (invading DNA is integrated into the host genome ...


Mechanisms Mediating The Pro-Fibrotic Effects Of Estradiol, Haley Fulton Nov 2018

Mechanisms Mediating The Pro-Fibrotic Effects Of Estradiol, Haley Fulton

Georgia Undergraduate Research Conference (GURC)

Scleroderma is a connective tissue disease which promotes fibrosis of the skin and internal organs. Scleroderma patients have upregulated production of fibrotic extracellular matrix proteins and upregulated serum estradiol levels compared to control subjects. Estradiol effects eukaryotic transcription and gene expression when it enters the nucleus via one of its nuclear hormone receptors. Estrogen receptor one/ estrogen receptor alpha (ESR1) is present on the nuclear membrane and binds the ligand estrogen. These experiments were designed to study the various isoforms of ESR1 and determine if gene expression of ESR1 was greater in fibroblasts and human skin stimulated with estradiol than ...