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Wayne State University Dissertations

5-methylcytosine, Activation induced deaminase, APOBEC3A, APOBEC3G, cytosine deamination, DNA demethylation

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Genetic And Biochemical Studies Of Human Apobec Family Of Proteins, Priyanga Wijesinghe Jan 2012

Genetic And Biochemical Studies Of Human Apobec Family Of Proteins, Priyanga Wijesinghe

Wayne State University Dissertations

The AID/APOBEC family of proteins in higher vertebrates converts cytosines in DNA or RNA into uracil. These proteins have essential roles in either innate immunity or adaptive immunity. Recently, AID has also been implicated in DNA demethylation in the context of early embryogenesis in mammals. This is partly based on the reported ability of AID to deaminate 5-methyl cytosine to thymine (5mC to T). I reexamined this proposed new role of AID (5mC deamination) with two members of the APOBEC family in a novel Escherichia coli based genetic system. My results confirmed that while all three enzymes are strong ...