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University of Massachusetts Medical School Faculty Publications

2000

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Full-Text Articles in Molecular Biology

Rnai: Double-Stranded Rna Directs The Atp-Dependent Cleavage Of Mrna At 21 To 23 Nucleotide Intervals, Phillip D. Zamore, Thomas Tuschl, Phillip A. Sharp, David P. Bartel Mar 2000

Rnai: Double-Stranded Rna Directs The Atp-Dependent Cleavage Of Mrna At 21 To 23 Nucleotide Intervals, Phillip D. Zamore, Thomas Tuschl, Phillip A. Sharp, David P. Bartel

University of Massachusetts Medical School Faculty Publications

Double-stranded RNA (dsRNA) directs the sequence-specific degradation of mRNA through a process known as RNA interference (RNAi). Using a recently developed Drosophila in vitro system, we examined the molecular mechanism underlying RNAi. We find that RNAi is ATP dependent yet uncoupled from mRNA translation. During the RNAi reaction, both strands of the dsRNA are processed to RNA segments 21-23 nucleotides in length. Processing of the dsRNA to the small RNA fragments does not require the targeted mRNA. The mRNA is cleaved only within the region of identity with the dsRNA. Cleavage occurs at sites 21-23 nucleotides apart, the same interval ...