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Molecular Biology Commons

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UMass Metabolic Network Publications

Biochemistry

Mycobacterium tuberculosis

Publication Year

Articles 1 - 3 of 3

Full-Text Articles in Molecular Biology

Role Of Granulocyte-Macrophage Colony-Stimulating Factor Production By T Cells During Mycobacterium Tuberculosis Infection, Alissa C. Rothchild, Britni L. Stowell, Girija Goyal, Claudio Nunes-Alves, Qianting Yang, Kadamba Papavinasasundaram, Christopher M. Sassetti, Glenn Dranoff, Xinchun Chen, Jinhee Lee, Samuel M. Behar Oct 2017

Role Of Granulocyte-Macrophage Colony-Stimulating Factor Production By T Cells During Mycobacterium Tuberculosis Infection, Alissa C. Rothchild, Britni L. Stowell, Girija Goyal, Claudio Nunes-Alves, Qianting Yang, Kadamba Papavinasasundaram, Christopher M. Sassetti, Glenn Dranoff, Xinchun Chen, Jinhee Lee, Samuel M. Behar

UMass Metabolic Network Publications

Mice deficient for granulocyte-macrophage colony-stimulating factor (GM-CSF(-/-)) are highly susceptible to infection with Mycobacterium tuberculosis, and clinical data have shown that anti-GM-CSF neutralizing antibodies can lead to increased susceptibility to tuberculosis in otherwise healthy people. GM-CSF activates human and murine macrophages to inhibit intracellular M. tuberculosis growth. We have previously shown that GM-CSF produced by iNKT cells inhibits growth of M. tuberculosis However, the more general role of T cell-derived GM-CSF during infection has not been defined and how GM-CSF activates macrophages to inhibit bacterial growth is unknown. Here we demonstrate that, in addition to nonconventional T cells, conventional T ...


Structural And Genetic Analyses Of The Mycobacterium Tuberculosis Protein Kinase B Sensor Domain Identify A Potential Ligand-Binding Site, Daniil M. Prigozhin, Kadamba Papavinasasundaram, Christina E. Baer, Kenan C. Murphy, Alisa Moskaleva, Tony Y. Chen, Tom Alber, Christopher M. Sassetti Oct 2016

Structural And Genetic Analyses Of The Mycobacterium Tuberculosis Protein Kinase B Sensor Domain Identify A Potential Ligand-Binding Site, Daniil M. Prigozhin, Kadamba Papavinasasundaram, Christina E. Baer, Kenan C. Murphy, Alisa Moskaleva, Tony Y. Chen, Tom Alber, Christopher M. Sassetti

UMass Metabolic Network Publications

Monitoring the environment with serine/threonine protein kinases is critical for growth and survival of Mycobacterium tuberculosis, a devastating human pathogen. Protein kinase B (PknB) is a transmembrane serine/threonine protein kinase that acts as an essential regulator of mycobacterial growth and division. The PknB extracellular domain (ECD) consists of four repeats homologous to penicillin-binding protein and serine/threonine kinase associated (PASTA) domains, and binds fragments of peptidoglycan. These properties suggest that PknB activity is modulated by ECD binding to peptidoglycan substructures, however, the molecular mechanisms underpinning PknB regulation remain unclear. In this study, we report structural and genetic characterization ...


Fine-Tuning Of Substrate Affinity Leads To Alternative Roles Of Mycobacterium Tuberculosis Fe2+-Atpases, Sarju J. Patel, Brianne E. Lewis, Jarukit E. Long, Subhalaxmi Nambi, Christopher M. Sassetti, Timothy L. Stemmler, Jose M. Arguello May 2016

Fine-Tuning Of Substrate Affinity Leads To Alternative Roles Of Mycobacterium Tuberculosis Fe2+-Atpases, Sarju J. Patel, Brianne E. Lewis, Jarukit E. Long, Subhalaxmi Nambi, Christopher M. Sassetti, Timothy L. Stemmler, Jose M. Arguello

UMass Metabolic Network Publications

Little is known about iron efflux transporters within bacterial systems. Recently, the participation of Bacillus subtilis PfeT, a P1B4-ATPase, in cytoplasmic Fe(2+) efflux has been proposed. We report here the distinct roles of mycobacterial P1B4-ATPases in the homeostasis of Co(2+) and Fe(2+) Mutation of Mycobacterium smegmatis ctpJ affects the homeostasis of both ions. Alternatively, an M. tuberculosis ctpJ mutant is more sensitive to Co(2+) than Fe(2+), whereas mutation of the homologous M. tuberculosis ctpD leads to Fe(2+) sensitivity but no alterations in Co(2+) homeostasis. In vitro, the three enzymes are activated by both ...