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UMass Metabolic Network Publications

Biochemistry

ATPase

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Full-Text Articles in Molecular Biology

Fine-Tuning Of Substrate Affinity Leads To Alternative Roles Of Mycobacterium Tuberculosis Fe2+-Atpases, Sarju J. Patel, Brianne E. Lewis, Jarukit E. Long, Subhalaxmi Nambi, Christopher M. Sassetti, Timothy L. Stemmler, Jose M. Arguello May 2016

Fine-Tuning Of Substrate Affinity Leads To Alternative Roles Of Mycobacterium Tuberculosis Fe2+-Atpases, Sarju J. Patel, Brianne E. Lewis, Jarukit E. Long, Subhalaxmi Nambi, Christopher M. Sassetti, Timothy L. Stemmler, Jose M. Arguello

UMass Metabolic Network Publications

Little is known about iron efflux transporters within bacterial systems. Recently, the participation of Bacillus subtilis PfeT, a P1B4-ATPase, in cytoplasmic Fe(2+) efflux has been proposed. We report here the distinct roles of mycobacterial P1B4-ATPases in the homeostasis of Co(2+) and Fe(2+) Mutation of Mycobacterium smegmatis ctpJ affects the homeostasis of both ions. Alternatively, an M. tuberculosis ctpJ mutant is more sensitive to Co(2+) than Fe(2+), whereas mutation of the homologous M. tuberculosis ctpD leads to Fe(2+) sensitivity but no alterations in Co(2+) homeostasis. In vitro, the three enzymes are activated by both ...