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Ray Enke Ph.D.

Articles 1 - 12 of 12

Full-Text Articles in Molecular Biology

In Silico Modeling Of Epigenetic-Induced Changes In Photoreceptor Cis-Regulatory Elements, Reafa A. Hossain, Nicholas R. Dunham, Raymond A. Enke, Christopher E. Berndsen Dec 2017

In Silico Modeling Of Epigenetic-Induced Changes In Photoreceptor Cis-Regulatory Elements, Reafa A. Hossain, Nicholas R. Dunham, Raymond A. Enke, Christopher E. Berndsen

Ray Enke Ph.D.

No abstract provided.

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Rna Sequencing Analysis Of The Developing Chicken Retina, Christophe Langouet-Astrie*, Annamarie Meinsen*, Emily R. Grunwald*, Stephen Turner, Raymond A. Enke Nov 2016

Rna Sequencing Analysis Of The Developing Chicken Retina, Christophe Langouet-Astrie*, Annamarie Meinsen*, Emily R. Grunwald*, Stephen Turner, Raymond A. Enke

Ray Enke Ph.D.

RNA sequencing transcriptome analysis using massively parallel next generation sequencing technology provides the capability to understand global changes in gene expression throughout a range of tissue samples. Development of the vertebrate retina requires complex temporal orchestration of transcriptional activation and repression. The chicken embryo (Gallus gallus) is a classic model system for studying developmental biology and retinogenesis. Existing retinal transcriptome projects have been critical to the vision research community for studying aspects of murine and human retinogenesis, however, there are currently no publicly available data sets describing the developing chicken retinal transcriptome. Here we used Illumina RNA sequencing …

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Connecting Common Genetic Polymorphisms To Protein Function: A Modular Project Sequence For Lecture Or Lab, Christopher E. Berndsen, Byron H. Young, Quinlin Mccormick*, Raymond A. Enke Oct 2016

Connecting Common Genetic Polymorphisms To Protein Function: A Modular Project Sequence For Lecture Or Lab, Christopher E. Berndsen, Byron H. Young, Quinlin Mccormick*, Raymond A. Enke

Ray Enke Ph.D.

Single nucleotide polymorphisms (SNPs) in DNA can result in phenotypes where the biochemical basis may not be clear due to the lack of protein structures. With the growing number of modeling and simulation software available on the internet, students can now participate in determining how small changes in genetic information impact cellular protein structure and function. We have developed a modular series of activities to engage lab or lecture students in examining the basis for common phenotypes. The activities range from basic phenotype test- ing/observation to DNA sequencing and simulation of protein structure and dynamics. We provide as an exam- …

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Qpcr Analysis Of Differential Gene Expression (Wet Lab), Raymond A. Enke May 2016

Qpcr Analysis Of Differential Gene Expression (Wet Lab), Raymond A. Enke

Ray Enke Ph.D.

This class tested protocol will guide students through the steps for the following activities:
  • Analyze qPCR data to determine normalized gene expression for candidate genes identified from an RNA-Seq data set
  • Set up additional or repeat experimental qPCR reactions
  • Create a draft poster outlining the methods & data collected during the 6-week RNA-Seq analysis lab module

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1st Strand Cdna Synthesis From Total Rna (Wet Lab), Raymond A. Enke May 2016

1st Strand Cdna Synthesis From Total Rna (Wet Lab), Raymond A. Enke

Ray Enke Ph.D.

This class tested protocol will guide students through the steps for the following activities:
• Construct a cDNA library representative of total cellular messenger RNAs

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Dissection & Rna Isolation From Embryonic Chicken Retina (Wet Lab), Raymond A. Enke May 2016

Dissection & Rna Isolation From Embryonic Chicken Retina (Wet Lab), Raymond A. Enke

Ray Enke Ph.D.

This class tested protocol will guide students through the steps for the following activities:
  • Dissect retina & cornea from developing chicken embryos
  • Extract total cellular RNA from tissues for downstream gene expression analysis

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Rna Gel & Spectrophotometer Analysis (Wet Lab), Raymond A. Enke May 2016

Rna Gel & Spectrophotometer Analysis (Wet Lab), Raymond A. Enke

Ray Enke Ph.D.

This class tested protocol will guide students through the steps for the following activities:
  • Determine quantity & purity of total RNA using a spectrophotometer
  • Determine the quality of total RNA using native agarose gel electrophoresis (no formaldehyde required)

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Bitter Taste Receptor Genotype/Phenotype Lab: A Modular Project Sequence For Lecture Or Lab, Raymond A. Enke May 2016

Bitter Taste Receptor Genotype/Phenotype Lab: A Modular Project Sequence For Lecture Or Lab, Raymond A. Enke

Ray Enke Ph.D.


Single nucleotide polymorphisms (SNPs) in DNA can result in phenotypes where the biochemical basis may not be clear due to the lack of protein structures. With the growing number of modeling and simulation software available on the internet, students can now participate in determining how small changes in genetic information impact cellular protein structure and function. We have developed a modular series of activities to engage lab or lecture students in examining the basis for common phenotypes.  The activities range from basic phenotype testing/observation to DNA sequencing and simulation of protein structure and dynamics. We provide as an example study …

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The Demethylating Agent 5-Aza Reduces The Growth, Invasiveness, And Clonogenicity Of Uveal And Cutaneous Melanoma, F. Rajaii, L. Asnaghi, Raymond Enke, S. Merbs, J. Handa, C. Eberhart Jul 2014

The Demethylating Agent 5-Aza Reduces The Growth, Invasiveness, And Clonogenicity Of Uveal And Cutaneous Melanoma, F. Rajaii, L. Asnaghi, Raymond Enke, S. Merbs, J. Handa, C. Eberhart

Ray Enke Ph.D.

No abstract provided.

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Jmu 4-Va Grant: Characterizing Epigenetic Regulation Of Gene Experession During Development Of The Vertebrate Retina, Raymond A. Enke Dec 2013

Jmu 4-Va Grant: Characterizing Epigenetic Regulation Of Gene Experession During Development Of The Vertebrate Retina, Raymond A. Enke

Ray Enke Ph.D.

4-VA Funded Research Grant: Characterizing epigenetic regulation of gene experession during development of the vertebrate retina

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Conditional Knockdown Of Dna Methyltransferase-1 (Dnmt1) Reveals A Key Role Of Retinal Pigment Epithelium In Mammalian Photoreceptor Differentiation, I. O. Nasonkin, S. L. Merbs, K. Lazo, V. F. Oliver, M. Brooks, K. Patel, Raymond Enke, J. Nellissery, M. Jamrich, Y. Z. Le, K. Bharti, R. N. Fariss, R. A. Rachel, D. J. Zack, E. Rodriguez-Boulan, A. Swaroop Dec 2012

Conditional Knockdown Of Dna Methyltransferase-1 (Dnmt1) Reveals A Key Role Of Retinal Pigment Epithelium In Mammalian Photoreceptor Differentiation, I. O. Nasonkin, S. L. Merbs, K. Lazo, V. F. Oliver, M. Brooks, K. Patel, Raymond Enke, J. Nellissery, M. Jamrich, Y. Z. Le, K. Bharti, R. N. Fariss, R. A. Rachel, D. J. Zack, E. Rodriguez-Boulan, A. Swaroop

Ray Enke Ph.D.

No abstract provided.

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Small Rnas Prevent Transcription-Coupled Erosion Of Histone H3 Lysine 9 Methylation In Arabidopsis Thaliana, Raymond Enke, Z. Dong, J. Bender Dec 2010

Small Rnas Prevent Transcription-Coupled Erosion Of Histone H3 Lysine 9 Methylation In Arabidopsis Thaliana, Raymond Enke, Z. Dong, J. Bender

Ray Enke Ph.D.

No abstract provided.

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