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Full-Text Articles in Molecular Biology

The Role Of Molecular Motors In Peripheral Nerve Regeneration, Melissa D. Priest Jan 2018

The Role Of Molecular Motors In Peripheral Nerve Regeneration, Melissa D. Priest

Publicly Accessible Penn Dissertations

Following injury, axons of the peripheral nervous system have retained the capacity for regeneration. While it is well established that injury signals require molecular motors for their transport from the injury site to the nucleus, whether kinesin and dynein motors play additional roles in peripheral nerve regeneration is not well understood. Here we use genetic mutants of motor proteins in a zebrafish peripheral nerve regeneration model to visualize and define in vivo roles for kinesin and dynein. We find that both kinesin-1 and dynein are required for zebrafish peripheral nerve regeneration. While loss of kinesin-1 reduced the overall robustness of ...


Cellular Interactions During Motor Nerve Regeneration, Allison F. Rosenberg Jan 2014

Cellular Interactions During Motor Nerve Regeneration, Allison F. Rosenberg

Publicly Accessible Penn Dissertations

Vertebrate peripheral nerves can regenerate, enabling severed axons to reconnect with their original synaptic targets. The interactions between injured nerves with cells in their environment, as well as the functional significance of these interactions, have not been determined in vivo and in real time. Here we provide the first minute-by-minute account of cellular interactions between laser transected motor nerves, macrophages, and Schwann cells in live intact zebrafish using transgenic lines that label each cell type in vivo. We find that axon fragmentation triggers macrophage invasion into the nerve to engulf axonal debris, and that delaying nerve fragmentation in a Wlds ...


Multiple Conserved Enhancers Of The Osteoblast Master Transcription Factor, Runx2, Integrate Diverse Signaling Pathways To Direct Expression To Developing Bone, Christopher Weber Jan 2013

Multiple Conserved Enhancers Of The Osteoblast Master Transcription Factor, Runx2, Integrate Diverse Signaling Pathways To Direct Expression To Developing Bone, Christopher Weber

Publicly Accessible Penn Dissertations

The vertebrate skeleton forms via two distinct modes of ossification, membranous and endochondral. Osteoblasts are also heterogeneous in embryonic origin; bone formed by either mode can be derived from neural crest cells or mesoderm. In contrast, all bone develops via a common genetic pathway regulated by the transcription factor Runx2. Runx2 is required for bone formation, and haploinsufficiency in humans causes the skeletal syndrome cleidocranial dysplasia, demonstrating the importance of gene dosage. Despite the central role of Runx2 in directing bone formation, little is understood about how its expression is regulated in development. We took an unbiased approach to identify ...