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Publicly Accessible Penn Dissertations

2016

D-loop

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Full-Text Articles in Molecular Biology

Dna Disentangled: Roles For Sgs1 And Top3 In Rec-X Resolution And Replication Fork Restart, Mary Rebecca Glineburg Jan 2016

Dna Disentangled: Roles For Sgs1 And Top3 In Rec-X Resolution And Replication Fork Restart, Mary Rebecca Glineburg

Publicly Accessible Penn Dissertations

Homologous recombination (HR), becomes important for repair during replication where completion of DNA synthesis relies on recombination intermediate-mediated lesion bypass. For decades, Holliday Junctions (HJs) were considered the primary recombination intermediate utilized during this repair process, but increasing evidence points out two strong discrepancies: 1) X-structures, when present, are often biochemically inconsistent with being HJs, and 2) despite HR mutants being sensitive to numerous DNA damaging agents, most insults don’t result in X-structure accumulation, suggesting alternative HR pathways are at play. The Sgs1-Top3-Rmi1 (STR) complex, in Saccharomyces cerevisiae, is vital for maintaining genome integrity, and is known to resolve ...