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Full-Text Articles in Molecular Biology

Interplay Between P53 And Epigenetic Pathways In Cancer, Jiajun Zhu Jan 2016

Interplay Between P53 And Epigenetic Pathways In Cancer, Jiajun Zhu

Publicly Accessible Penn Dissertations

The human TP53 gene encodes the most potent tumor suppressor protein p53. More than half of all human cancers contain mutations in the TP53 gene, while the majority of the remaining cases involve other mechanisms to inactivate wild-type p53 function. In the first part of my dissertation research, I have explored the mechanism of suppressed wild-type p53 activity in teratocarcinoma. In the teratocarcinoma cell line NTera2, we show that wild-type p53 is mono-methylated at Lysine 370 and Lysine 382. These post-translational modifications contribute to the compromised tumor suppressive activity of p53 despite a high level of wild-type protein in NTera2 ...


Determination Of A Comprehensive Alternative Splicing Regulatory Network And The Combinatorial Regulation By Key Factors During The Epithelial To Mesenchymal Transition, Yueqin Yang Jan 2016

Determination Of A Comprehensive Alternative Splicing Regulatory Network And The Combinatorial Regulation By Key Factors During The Epithelial To Mesenchymal Transition, Yueqin Yang

Publicly Accessible Penn Dissertations

The epithelial to mesenchymal transition (EMT) is a process by which epithelial cells transdifferentiate into mesenchymal cells. It is essential for embryonic development and implicated in cancer metastasis. While the transcriptional regulation of EMT has been well-studied, the role of post-transcriptional regulation, particularly alternative splicing (AS) regulation in EMT, remains relatively uncharacterized. We previously showed that the epithelial cell-type-specific proteins ESRP1 and ESRP2 are important for regulation of many AS events that altered during EMT. However, the contributions of the ESRPs and other splicing regulators to the splicing regulatory network in EMT require further investigation.

In the first part of ...