Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 5 of 5
Full-Text Articles in Molecular Biology
The F-Box Protein Fbw7 Negatively Regulates The Stability Of Erk3 Protein, Nicole Walters
The F-Box Protein Fbw7 Negatively Regulates The Stability Of Erk3 Protein, Nicole Walters
Browse all Theses and Dissertations
Extracellular signal-regulated kinase 3 (ERK3) is a member of the atypical mitogen-activated protein kinase (MAPK) subfamily, whose members have been shown to play important roles in a number of cellular processes including proliferation, differentiation, migration, and apoptosis. While signals regulating ERK3 kinase activity remain unclear, ERK3 is known to be an unstable protein with function tightly regulated via ubiquitination and proteasomal turnover. The deubiquitinating enzyme USP20 has been shown to regulate ERK3 by stabilizing the kinase, but presently, no destabilizing ubiquitin ligases have been identified. The SKP1-CUL1-F-box protein (SCF) E3 ligases are a subfamily of ubiquitin E3 ligases composed of …
Erk3 Negatively Regulates The Il-6/Stat3 Signaling Via Socs3, Astha Shakya
Erk3 Negatively Regulates The Il-6/Stat3 Signaling Via Socs3, Astha Shakya
Browse all Theses and Dissertations
Mitogen activated protein kinases (MAPKs) are Ser/Thr kinases that relay the extracellular signal into intracellular responses and regulate several biological responses. They are classified into conventional MAPKs and atypical MAPKs. Extracellular signal regulated kinase 3 (ERK3) is an atypical MAPK that has a single phospho-acceptor site (Ser 189) in its activation motif instead of the canonical Thr-Xaa-Tyr (TXY) motif of conventional MAPK like ERK1/2. ERK3 comprises of a unique C terminal tail and a central C34 domain that further distinguishes it from ERK1/2. Moreover, compared to ERK1/2, much less is known about the upstream activators and the downstream targets of …
Role Of Erk3 In Regulating Rhogdi1-Paks Signaling Axis, Hitham Abdulrahman Aldharee
Role Of Erk3 In Regulating Rhogdi1-Paks Signaling Axis, Hitham Abdulrahman Aldharee
Browse all Theses and Dissertations
Extracellular signal-regulated kinase 3 (ERK3) is an atypical protein kinase of the mitogen-activated protein kinase (MAPK) family. In comparison to well-investigated ERK1/2 (classical) MAPKs, much less has been discovered about ERK3 signaling and its cellular functions. Recent studies have shown that ERK3 is overexpressed in various types of cancers such as lung cancer and breast cancer and that ERK3 promotes cancer cell migration and invasion. How ERK3 regulates cancer cell motility and invasiveness, however, is still largely unknown. RhoGTPases, including Rho, Cdc42 and Rac1, play critical roles in regulating cell motility and invasiveness through activating downstream effectors such as p21-activated …
Erk3 As A Braf-Regulated Tumor Suppressor Is A New Potential Cancer Target In Melanoma, Minyi Chen
Erk3 As A Braf-Regulated Tumor Suppressor Is A New Potential Cancer Target In Melanoma, Minyi Chen
Browse all Theses and Dissertations
Melanoma is the highest mortality rate skin cancer and the sixth most common cancer in the U.S..[1, 2] Arising from melanocytes, melanoma is known for frequent mutation of BRAF to its constitutive activation state BRAFV600E, thus over-activating its downstream MAPK targets ERK1/2 and contributing to melanoma progression.[3, 4, 5] ERK3, a less studied MAPK family protein which contributes to promoting lung, breast, cervical, head and neck (HNC) cancer metastasis, has also shown a correlation with upregulation of BRAF.[6, 7, 8, 9] However, the BRAF-ERK3 regulatory mechanism and the function of ERK3 in melanoma are still unclear. Here, we first elucidate …
Functional Characterization Of Cancer-Related Mutations Of Erk3, Hadel Mohammed Alsaran
Functional Characterization Of Cancer-Related Mutations Of Erk3, Hadel Mohammed Alsaran
Browse all Theses and Dissertations
Extracellular signal-regulated kinase 3 (ERK3) is an atypical member of the mitogen-activated protein kinase (MAPK) family. Recent studies have shown that ERK3 is highly upregulated in multiple cancers, such as lung cancer and colon cancer. Importantly, ERK3 promotes cancer cell migration and invasion by phosphorylating steroid receptor activator 3 (SRC-3), hence upregulating pro-invasive matrix metalloproteinase genes. While the link between ERK3 and cancers has been recognized, little is known about ERK3 mutations in cancer progression. In this study, we have investigated ERK3 mutations on arginine 64 (arginine 64 mutated to cysteine or histidine, R64C or R64H) and leucine 290 (leucine …