Open Access. Powered by Scholars. Published by Universities.®

Molecular Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 3 of 3

Full-Text Articles in Molecular Biology

Due-B In Chromatin And Nuclear Speckles, Nadia Katrangi Jan 2007

Due-B In Chromatin And Nuclear Speckles, Nadia Katrangi

Browse all Theses and Dissertations

The DNA unwinding element binding protein (DUE-B) was first identified by using a yeast one hybrid screen with the DNA unwinding element (DUE) from the c-myc origin as bait. DUE-B's orthologue in the yeast Saccharomyces cerevisiae lacks the last 60 C-terminal amino acids and has been identified as a D-tyrosyl-tRNA deacylase. A substantial group of evidence suggests a role for DUE-B in the regulation of replication initiation. Here we show that DUE-B is focused in nuclear speckles and colocalizes with spliceosome associated protein 145 (SAP145), an mRNA splicing factor 3B subunit. Mass spectrometry results show that SAP145 co-purifies with ...


Differential Effects Of Mutant Tap63Γ On Transactivation Of P53 And/Or P63 Responsive Genes And Their Effects On Global Gene Expression, Shama Khan Khokhar Jan 2007

Differential Effects Of Mutant Tap63Γ On Transactivation Of P53 And/Or P63 Responsive Genes And Their Effects On Global Gene Expression, Shama Khan Khokhar

Browse all Theses and Dissertations

p63, a member of the p53 gene family, known to play a role in development, has more recently also been implicated in cancer progression. Mice lacking p63 exhibit severe developmental defects such as limb truncations, abnormal skin, and absence of hair follicles, teeth, and mammary glands. Germline missense mutations of p63 have been shown to be responsible for several human developmental syndromes including SHFM, EEC and ADULT syndromes and are associated with anomalies in the development of organs of epithelial origin. The contrasting phenotypes associated with the different classes of p63 mutations might be in part due to the differential ...


Detection And Destruction Of Escherichia Coli Bacteria And Bacteriophage Using Biofunctionalized Nanoshells, Joseph E. Van Buren Jan 2007

Detection And Destruction Of Escherichia Coli Bacteria And Bacteriophage Using Biofunctionalized Nanoshells, Joseph E. Van Buren

Browse all Theses and Dissertations

The ability to detect chemical and biological agents is arguably one of the highest priority technical challenges today. The capability to obtain specific information at and near single-molecule resolution is the ultimate goal in chemical and biological agent detection. Metallic nanostructures, nanoshells and nanorods in particular, are attractive substrates because of their plasmonic properties. Combining the specificity of biomolecular recognition with these nanostructures might lead to increased sensitivity and selectivity. Localization of biological recognition motifs to the surface of these nanostructures could provide a mechanism for highly specific and directed energy transfer when bound to its target. This study utilizes ...