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Wright State University

Theses/Dissertations

Biochemistry

Publication Year

Articles 1 - 3 of 3

Full-Text Articles in Molecular Biology

Novel Insight Into The Role Of Lxrα In Metabolic Regulation Via Dna Binding As A Heterodimer With Pparα And As A Homodimer, Andrea M. Klingler Jan 2016

Novel Insight Into The Role Of Lxrα In Metabolic Regulation Via Dna Binding As A Heterodimer With Pparα And As A Homodimer, Andrea M. Klingler

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Liver X receptor a (LXRa) plays a critical role in the maintenance of energy homeostasis within a cell through tight transcriptional regulation of genes involved in metabolism of lipids, glucose, and cholesterol. Although LXRa has been established to function as a heterodimer with the retinoid X receptor a (RXRa), recent studies have determined that LXRa also interacts directly with peroxisome proliferator-activated receptor a (PPARa). However, little is known regarding the functionality of this heterodimer, if any exists at all. This study determined that a heterodimer of PPARa and LXRa is capable of binding to candidate response elements in vitro with ...


Differences In Fecal Metabolite Profiles From Geographically Distinct Populations Of Adolescents, Jessica Moncivaiz Jan 2015

Differences In Fecal Metabolite Profiles From Geographically Distinct Populations Of Adolescents, Jessica Moncivaiz

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Microbiota of the gastrointestinal tract have a variety of functions within the human body. They participate in protection of the host from pathogens, aid in immune system development and regulation, and carry out a variety of metabolic functions. This study focuses on the ability of gut microbiota to create metabolites through the degradation of food products. Using 1H NMR on fecal water extracts, I compared the metabolite profiles of two geographically distinct cohorts: healthy adolescents from Egypt (n=28) and healthy adolescents from the United States (n=14). Multivariate statistical analyses of binned NMR data confirmed that samples separated into ...


Lipase-Kinase Associations Involving Pld2, Jak3 And Fes That Underlie Cancer Cell Proliferation And Invasion, Qing Ye Jan 2012

Lipase-Kinase Associations Involving Pld2, Jak3 And Fes That Underlie Cancer Cell Proliferation And Invasion, Qing Ye

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Phospholipase D (PLD) is an enzyme that breaks down phospholipids in the cell membrane. It has been suggested that PLD may play a role during cell proliferation and cell invasion of cancer cells. The objective of this thesis was to define new molecular signaling pathways in which PLD2 might be involved in terms of cell proliferation (first part) and cell invasion (second part). To this, I compared molecular and biochemical aspects between untransformed cell lines with highly invasive, transformed breast cancer cells. In the first part, I investigated the interaction of two tyrosine kinases with PLD2 and the effect of ...