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Ether Bridge Formation And Chemical Diversification In Loline Alkaloid Biosynthesis, Juan Pan
Ether Bridge Formation And Chemical Diversification In Loline Alkaloid Biosynthesis, Juan Pan
Theses and Dissertations--Plant Pathology
Loline alkaloids, found in many grass-Epichloë symbiota, are toxic or feeding deterrent to invertebrates. The loline alkaloids all share a saturated pyrrolizidine ring with a 1-amine group and an ether bridge linking C2 and C7. The steps in biosynthesis of loline alkaloids are catalyzed by enzymes encoded by a gene cluster, designated LOL, in the Epichloë genome. This dissertation addresses the enzymatic, genetic and evolutionary basis for diversification of these alkaloids, focusing on ether bridge formation and the subsequent modifications of the 1-amine to form different loline alkaloids.
Through gene complementation of a natural lolO mutant and comparison …