Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Molecular Biology
A Reliable And High Yielding Method For Isolation Of Genomic Dna From Ammi Majus., Sandip S. Magdum
A Reliable And High Yielding Method For Isolation Of Genomic Dna From Ammi Majus., Sandip S. Magdum
Sandip S. Magdum
The developed protocol describes a cheaper, quicker and reliable method for the isolation of pure DNA from medicinal herbs, such as Ammi majus, which produces the secondary metabolites xanthotoxin and berganpectane having immense medicinal importance. Use of CTAB, liquid nitrogen and EDTA in different isolation protocols analyzed for A. majus, all were ended with polysaccharide and protein contamination with low purity of DNA (A260/280 = 1.3 – 1.6), revealed a need for method modification for the inexpensive and rapid isolation of pure DNA. Developed reliable and competent protocol isolated enough pure DNA (A260/280 = 1.81) without following time consuming lengthy …
Effect Of Agrobacterium Induced Necrosis, Antibiotic Induced Phytotoxicity And Other Factors In Successful Plant Transformation., Sandip S. Magdum
Effect Of Agrobacterium Induced Necrosis, Antibiotic Induced Phytotoxicity And Other Factors In Successful Plant Transformation., Sandip S. Magdum
Sandip S. Magdum
Agrobacterium tumefaciens infection and antibiotic wash are the critical steps of Agrobacterium mediated plant transformation procedure, most time responsible for lower transformation efficiency due to necrosis and phytotoxicity caused by biotic stress of Agrobacterium and abiotic stress by antibiotics respectively. Ammi majus Egyptian origin medicinal plant and Pearl millet cereal grain crop were studied for their stress responses to Agrobacterium mediated transformation (AMT). Agrobacterium strains LBA4404 (O.D.=0.6-0.8) and EHA105 (O.D.=0.2-0.4) were used for transformation experiments to infect calli of Ammi majus and embryogenic calli of Pearl millet respectively. Incase of antibiotic wash, Cefotaxime 500 mg L-1 was used for LBA4404 …