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Kinetics Of Hiv-1 Uncoating In C20 Microglial Cells, Melanie Anne Taylor
Kinetics Of Hiv-1 Uncoating In C20 Microglial Cells, Melanie Anne Taylor
MSU Graduate Theses
Uncoating is a poorly understood yet required step of HIV-1 replication that is defined as the disassembly of the viral capsid structure. The goal of this project is to characterize uncoating in C20 microglial cells. These cells are a natural target of HIV-1 that are infected to establish latent viral reservoirs and HIV-associated neurological disorders. A stable C20 cell line that expresses TRIM-CypA was established to study the kinetics of uncoating with the CsA washout assay. The expression of TRIM-CypA was confirmed by western blot and the functionality of the protein was confirmed by a viral infectivity assay. Using this …