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Genetics

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2008

Articles 1 - 1 of 1

Full-Text Articles in Molecular Biology

Rna Polymerase Ii-Mediated Transcription At Active Loci Does Not Require Histone H3s10 Phosphorylation In Drosophila, Weili Cai, Xiaomin Bao, Huai Deng, Ye Jin, Jack Girton, Jorgen Johansen, Kristen M. Johansen Jan 2008

Rna Polymerase Ii-Mediated Transcription At Active Loci Does Not Require Histone H3s10 Phosphorylation In Drosophila, Weili Cai, Xiaomin Bao, Huai Deng, Ye Jin, Jack Girton, Jorgen Johansen, Kristen M. Johansen

Biochemistry, Biophysics and Molecular Biology Publications

JIL-1 is the major kinase controlling the phosphorylation state of histone H3S10 at interphase in Drosophila. In this study, we used three different commercially available histone H3S10 phosphorylation antibodies, as well as an acid-free polytene chromosome squash protocol that preserves the antigenicity of the histone H3S10 phospho-epitope, to examine the role of histone H3S10 phosphorylation in transcription under both heat shock and non-heat shock conditions. We show that there is no redistribution or upregulation of JIL-1 or histone H3S10 phosphorylation at transcriptionally active puffs in such polytene squash preparations after heat shock treatment. Furthermore, we provide evidence that heat shock-induced ...