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Full-Text Articles in Molecular Biology

Crispr-Assisted Interrogation And Engineering Of Metabolic Pathways, Marcelo Colika Bassalo Jan 2018

Crispr-Assisted Interrogation And Engineering Of Metabolic Pathways, Marcelo Colika Bassalo

Molecular, Cellular, and Developmental Biology Graduate Theses & Dissertations

Since Mendel’s work established the basis of inheritance in the late 1800s, multiple decades of research characterized monogenic traits across all domains of life. Yet, we still have a fairly limited knowledge on the genotype behind the vast majority of phenotypes. It is now evident that discrete biological functions can rarely be linked to a single gene. Further, these multigenic traits are often interconnected via a sophisticated and robust metabolic and regulatory network, selected by evolution in order to optimally distribute resources. The complexity of these multigenic traits challenges traditional genetic tools, broadly limiting our capability to understand and ...


Mirnas Collaborate With A Conserved Rna Binding Protein To Ensure Development And Stress Response In C. Elegans, Rebecca A. Zabinsky Jan 2015

Mirnas Collaborate With A Conserved Rna Binding Protein To Ensure Development And Stress Response In C. Elegans, Rebecca A. Zabinsky

Molecular, Cellular, and Developmental Biology Graduate Theses & Dissertations

miRNAs play critical roles in development and other cellular processes in C. elegans even though most individual miRNAs are not essential for development or viability (Miska et al., 2007). Extensive studies in the field have suggested that most miRNA functions are executed through complex miRNA-target interaction networks. Furthermore, such networks may also function semi-redundantly with other regulatory systems to shape gene expression dynamics for proper physiological functions. Hypothesizing that miRNAs function in stress conditions, I collaborated with a postdoctoral fellow to investigate the role of a specific miRNA miR-71 in starvation induced L1 diapause. Hypothesizing that miRNAs collaborate with other ...


Regulation Of C. Elegans Developmental Timing By The Gata Transcription Factor Elt-1, Max Louis Cohen Jan 2013

Regulation Of C. Elegans Developmental Timing By The Gata Transcription Factor Elt-1, Max Louis Cohen

Molecular, Cellular, and Developmental Biology Graduate Theses & Dissertations

The heterochronic gene network controls developmental timing in the nematode roundworm Caenorhabditis elegans. Bi-stable switch-like changes in gene expression occur during its development as stage-specific microRNAs are expressed and subsequently down-regulate other stage-specific factors, allowing for developmental progression. Key genes in this regulatory network are phylogenetically conserved and include the post-transcriptional microRNA repressor lin-28; the nuclear hormone receptor daf-12; and the microRNAs lin-4, mir-48, mir-84, mir-241, and let-7. daf-12 is the only factor currently known to regulate transcription of the Let-7 microRNA family, but its contribution is insufficient to account for all of the transcriptional regulation observed. In this work ...


Paternal Mitochondria Elimination And Cell Death Regulation In C. Elegans, Qinghua Zhou Jan 2012

Paternal Mitochondria Elimination And Cell Death Regulation In C. Elegans, Qinghua Zhou

Molecular, Cellular, and Developmental Biology Graduate Theses & Dissertations

The nematode C. elegans is a model organism for various biomedical studies due to its genetic simplicity and conservation with humans. As C. elegans is uniquely amenable to cellular, molecular genetic, and biochemical analyses, many basic biological processes are investigated by experimentally manipulating and observing worms. My thesis describes how I used C. elegans models to advance our understanding of paternal mitochondria elimination and cell death regulation.

Mitochondria are membrane-enclosed organelles that carry their own genome (mtDNA). In mammals, the inheritance of mitochondria and mtDNA is strictly maternal, despite the fact that a sperm can inject up to 100 functional ...