Open Access. Powered by Scholars. Published by Universities.®

Molecular Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

Genetics

Iowa State University

Keyword
Publication Year
Publication
Publication Type

Articles 1 - 30 of 98

Full-Text Articles in Molecular Biology

Aptamers For Diagnostics With Applications For Infectious Diseases, Muslum Ilgu, Rezzan Fazlioglu, Meric Ozturk, Yasemin Ozsurekci, Marit Nilsen-Hamilton Apr 2019

Aptamers For Diagnostics With Applications For Infectious Diseases, Muslum Ilgu, Rezzan Fazlioglu, Meric Ozturk, Yasemin Ozsurekci, Marit Nilsen-Hamilton

Biochemistry, Biophysics and Molecular Biology Publications

Aptamers are in vitro selected oligonucleotides (DNA, RNA, oligos with modified nucleotides) that can have high affinity and specificity for a broad range of potential targets with high affinity and specificity. Here we focus on their applications as biosensors in the diagnostic field, although they can also be used as therapeutic agents. A small number of peptide aptamers have also been identified. In analytical settings, aptamers have the potential to extend the limit of current techniques as they offer many advantages over antibodies and can be used for real-time biomarker detection, cancer clinical testing, and detection of infectious microorganisms and ...


Using Genomic And Gene Expression Methods To Understand The Phenotypic Response Of Soybean To Sudden Death Syndrome Caused By Fusarium Virguliforme, Alexander Stephen Luckew Jan 2018

Using Genomic And Gene Expression Methods To Understand The Phenotypic Response Of Soybean To Sudden Death Syndrome Caused By Fusarium Virguliforme, Alexander Stephen Luckew

Graduate Theses and Dissertations

Soybean sudden death syndrome (SDS) is one of the most important soybean diseases in the Midwestern United States, leading to average losses of $396 million per year from 1996 to 2015. The causal agent of SDS, soilborne fungus Fusarium virguliforme (Fv), causes root rot symptoms and releases phytotoxins taken up by the plant to produce chlorosis and necrosis in the leaves. The main management practice used to reduce the impact of SDS is planting resistant soybean cultivars. Resistance to SDS is known to be quantitative with 88 known quantitative trait loci (QTL) based on Soybase.org. Many of these QTL ...


Molecular Mechanisms Governing Plant Parasitic Nematode Signaling And Host Parasitism, Stacey Nicola Barnes Jan 2018

Molecular Mechanisms Governing Plant Parasitic Nematode Signaling And Host Parasitism, Stacey Nicola Barnes

Graduate Theses and Dissertations

Pathogen infection of crops causes large-scale annual yield losses for farmers worldwide and hinders global efforts to provide adequate amounts of nutrition for the ever-growing human population. Plant-parasitic nematodes (PPN) are among some of the most devastating pathogens due to their ability to parasitize an expansive variety of agriculturally important crops. In order to identify ways to attenuate PPN infection and limit yield losses it is vital that we increase our understanding of host-PPN interactions. Here we investigate the molecular mechanisms that are occurring both within PPN and at the interface between PPN and their host plants.

Research into PPN-derived ...


Investigations Into Nutritional Quality, Commercial Productivity, And Genetic Purity Of Maize Grain For Organic Production Systems, Ryan Huffman Jan 2017

Investigations Into Nutritional Quality, Commercial Productivity, And Genetic Purity Of Maize Grain For Organic Production Systems, Ryan Huffman

Graduate Theses and Dissertations

Consumer demand for organic maize grain has steadily increased in the past decade which has resulted in an increased interest for this market class by plant breeders and geneticist. Each chapter in this dissertation investigates areas of concern to the organic community and possible solutions for improvement. Chapter 2 seeks to understand which combinations of genetic mechanisms are capable of a further increase in the methionine concentration of maize grain over individual mechanisms. Based on the genetic mechanisms evaluated, it was concluded that crosses combining dzr1 and recurrent selection in hybrid combination can elevate methionine concentration and overall grain nutritional ...


Insights Into Retinal Cell Fate Determination In Vertebrates Using Transcriptomic Profiling And Genome Editing, Rebecca Chowdhury Jan 2017

Insights Into Retinal Cell Fate Determination In Vertebrates Using Transcriptomic Profiling And Genome Editing, Rebecca Chowdhury

Graduate Theses and Dissertations

Deciphering the mechanisms of development of retinal neurons is not only of immense interest to developmental biologists, but is also vital for regenerative therapeutic applications. To attain this goal, it is critical to understand how specific intrinsic factors control cell fate decisions and neuronal maturation processes. In the retina, Atoh7 is a highly conserved transcription factor that is essential for retinal ganglion cell development in the developing mouse and zebrafish. Atoh7 labels a subset of cells in the developing retina that are progressing from a progenitor to a differentiated state. To capture cells during the window when the cell fate ...


On The Molecular Biology And Evolution Of Plant Parasitism By Nematodes, Jason Brett Noon Jan 2016

On The Molecular Biology And Evolution Of Plant Parasitism By Nematodes, Jason Brett Noon

Graduate Theses and Dissertations

Plant-parasitic nematodes (PPN) are among the most devastating plant pathogens. However, our understanding of how nematodes adapted to plant parasitism, and the molecular mechanisms that PPN use during infection is limited. Among the most important genomic changes that occurred in the free-living nematode ancestors of PPN were multiple horizontal gene transfer (HGT) events from bacteria. Though it is clear that HGT helped shape the genomes of many PPN, how this process occurred is unknown. Also, it is evident that successful parasitism occurs from the delivery of proteinaceous effectors into plant roots to hijack and modify host cellular processes.

The research ...


Detecting Cells In Time Varying Intensity Images In Confocal Microscopy For Gene Expression Studies In Living Cells, Debasis Mitra, Rostyslav Boutchko, Judhajeet Ray, Marit Nilsen-Hamilton Jan 2015

Detecting Cells In Time Varying Intensity Images In Confocal Microscopy For Gene Expression Studies In Living Cells, Debasis Mitra, Rostyslav Boutchko, Judhajeet Ray, Marit Nilsen-Hamilton

Biochemistry, Biophysics, and Molecular Biology Presentations and Posters

In this work we present a time-lapsed confocal microscopy image analysis technique for an automated gene expression study of multiple single living cells. Fluorescence Resonance Energy Transfer (FRET) is a technology by which molecule-to-molecule interactions are visualized. We analyzed a dynamic series of ~102 images obtained using confocal microscopy of fluorescence in yeast cells containing RNA reporters that give a FRET signal when the gene promoter is activated. For each time frame, separate images are available for three spectral channels and the integrated intensity snapshot of the system. A large number of time-lapsed frames must be analyzed to identify ...


The Molecular Mechanisms Of Brassinosteroid-Regulated Drought Stress Response In Arabidopsis Thaliana, Buyun Tang Jan 2015

The Molecular Mechanisms Of Brassinosteroid-Regulated Drought Stress Response In Arabidopsis Thaliana, Buyun Tang

Graduate Theses and Dissertations

Brassinosteroids (BRs) are involved in diverse developmental processes such as cell elongation, vascular differentiation, senescence and stress response. The mechanisms and regulatory networks of BR-regulated plant growth and development have been well described for the past decade with the identification of receptors, kinases and central transcription factors involved in BR signaling. Recent studies revealed BRs also extensively participated in plant response to environmental stresses, although the mechanisms of BR-regulated stress response is largely unknown. Coordination of plant growth and stress response requires integration of multiple signaling output through hormonal crosstalk. Studies of BR signaling pathway and BR-mediated physiological responses indicate ...


Epigenetic Interplay Between Histone H3k9me2 And Jil-1 Mediated Histone H3s10ph, Chao Wang Jan 2014

Epigenetic Interplay Between Histone H3k9me2 And Jil-1 Mediated Histone H3s10ph, Chao Wang

Graduate Theses and Dissertations

In Drosophila, JIL-1 Kinase specifically localizes to euchromatic interband regions of polytene chromosomes and is responsible for histone H3S10 phosphorylation at interphase. Previous genetic interaction assays have demonstrated that the JIL-1 protein can counterbalance the effect of the major heterochromatin components Su(var)3-7 on position-effect variegation (PEV). In this study, we show that the haplo-enhancer effect of JIL-1 has the ability to counterbalance the haplo-suppressor effect of two other major heterochromatin components, Su(var)3-9 and Su(var)2-5, on position-effect variegation, providing evidence that a finely tuned balance between the levels of JIL-1 and the major heterochromatin components ...


Role Of A Gamma-Aminobutryic Acid (Gaba) Receptor Mutation In The Evolution And Spread Of Diabrotica Virgifera Virgifera Resistance To Cyclodiene Insecticides, H. Wang, Brad S. Coates, H. Chen, Thomas W. Sappington, T. Guillemaud, B. D. Siegfried Oct 2013

Role Of A Gamma-Aminobutryic Acid (Gaba) Receptor Mutation In The Evolution And Spread Of Diabrotica Virgifera Virgifera Resistance To Cyclodiene Insecticides, H. Wang, Brad S. Coates, H. Chen, Thomas W. Sappington, T. Guillemaud, B. D. Siegfried

Entomology Publications

The western corn rootworm, Diabrotica virgifera virgifera, is a damaging pest of cultivated corn that was controlled by applications of cyclodiene insecticides from the late 1940s until resistance evolved ∼10 years later. Range expansion from the western plains into eastern USA coincides with resistance development. An alanine to serine amino acid substitution within the Rdl subunit of the gamma-aminobutyric acid (GABA) receptor confers resistance to cyclodiene insecticides in many species. We found that the non-synonymous single nucleotide polymorphism (SNP) G/T at the GABA receptor cDNA position 838 (G/T838) of D. v. virgifera resulted in the alanine to ...


Genetic Parameters For Concentrations Of Minerals In Longissimus Muscle And Their Associations With Palatability Traits In Angus Cattle, R. G. Mateescu, A. J. Garmyn, Richard G. Tait Jr., Qing Duan, Q. Liu, Mary S. Mayes, Dorian J. Garrick, A. L. Van Eenennaam, D. L. Vanoverbeke, G. G. Hilton, Donald C. Beitz, James M. Reecy Mar 2013

Genetic Parameters For Concentrations Of Minerals In Longissimus Muscle And Their Associations With Palatability Traits In Angus Cattle, R. G. Mateescu, A. J. Garmyn, Richard G. Tait Jr., Qing Duan, Q. Liu, Mary S. Mayes, Dorian J. Garrick, A. L. Van Eenennaam, D. L. Vanoverbeke, G. G. Hilton, Donald C. Beitz, James M. Reecy

Animal Science Publications

The objective of this study was to estimate genetic parameters for concentrations of minerals in LM and to evaluate their associations with beef palatability traits. Samples of LM from 2,285 Angus cattle were obtained and fabricated into steaks for analysis of mineral concentrations and for trained sensory panel assessments. Nine minerals, including calcium, copper, iron, magnesium, manganese, phosphorus, potassium, sodium, and zinc, were quantified. Restricted maximum likelihood procedures were used to obtain estimates of variance and covariance components under a multiple-trait animal model. Estimates of heritability for mineral concentrations in LM varied from 0.01 to 0.54. Iron ...


The Effects Of Heat Stress In Redox Balance And Inflammatory Signaling In Porcine Skeletal Muscle, Sandra Isabel Rosado Montilla Jan 2013

The Effects Of Heat Stress In Redox Balance And Inflammatory Signaling In Porcine Skeletal Muscle, Sandra Isabel Rosado Montilla

Graduate Theses and Dissertations

Heat stress (HS) is a major concern for human health as well as for the swine industry. In 2012, HS resulted in the largest number of weather related human fatalities in the US. Animal production is also compromised as HS leads to poor sow performance, decreased carcass value and increased veterinary costs. However, the molecular effects of HS on skeletal muscle are still unclear. This study aimed to determine the extent to which HS disrupted redox balance and initiated an inflammatory response in oxidative and glycolytic porcine skeletal muscle. Moreover, we quantified the contribution of reduced feed intake to the ...


Hybrid Breeding In Perennial Grasses Based On Self-Incompatibility And Self-Fertility, Andrea Sofia Arias Aguirre Jan 2013

Hybrid Breeding In Perennial Grasses Based On Self-Incompatibility And Self-Fertility, Andrea Sofia Arias Aguirre

Graduate Theses and Dissertations

For a long time, petroleum-based fuels have been used to provide energy, but limited supplies along with environmental effects of fossil fuels created the need of alternative and clean energy sources. Grasses in general have a low lignin content compared to woody species which is desirable for ethanol production. Furthermore, other features such as: low water and nutrient requirements, and a high rate of carbon fixation that make grasses desirable for biomass production. Perennial grasses have also environmental advantages such as reduced soil organic matter and tillage requirements compared with annual species. Perennial ryegrass has been increasingly used in Europe ...


Investigation Of The Arabidopsis Nonhost Resistance Mechanism Against The Soybean Pathogen, Phytophthora Sojae, Rishi Sumit Jan 2013

Investigation Of The Arabidopsis Nonhost Resistance Mechanism Against The Soybean Pathogen, Phytophthora Sojae, Rishi Sumit

Graduate Theses and Dissertations

Nonhost resistance (NHR) provides immunity to all members of a plant species against all isolates of a microorganism that is pathogenic to other plant species. Three Arabidopsis thaliana PEN (penetration deficient) genes, PEN1, 2 and 3 have been shown to provide prehaustorial NHR against the barley pathogen Blumeria graminis f. sp. hordei. Arabidopsis pen1-1 mutant is penetrated by the hemibiotrophic oomycete pathogen, Phytophthora sojae that causes root and stem rot disease in soybean. The P. sojae susceptible (pss) 1 mutant is infected by both P. sojae and the hemibiotrophic fungal pathogen, Fusarium virguliforme that causes sudden death syndrome in soybean ...


Genetic Parameters For Carnitine, Creatine, Creatinine, Carnosine, And Anserine Concentration In Longissimus Muscle And Their Association With Palatability Traits In Angus Cattle, R. G. Mateescu, A. J. Garmyn, M. A. O'Neil, Richard G. Tait Jr., Almass A. Abuzaid, Mary S. Mayes, Dorian J. Garrick, A. L. Van Eenennaam, D. L. Vanoverbeke, G. G. Hilton, Donald C. Beitz, James M. Reecy Dec 2012

Genetic Parameters For Carnitine, Creatine, Creatinine, Carnosine, And Anserine Concentration In Longissimus Muscle And Their Association With Palatability Traits In Angus Cattle, R. G. Mateescu, A. J. Garmyn, M. A. O'Neil, Richard G. Tait Jr., Almass A. Abuzaid, Mary S. Mayes, Dorian J. Garrick, A. L. Van Eenennaam, D. L. Vanoverbeke, G. G. Hilton, Donald C. Beitz, James M. Reecy

Animal Science Publications

The objective of this study was to estimate genetic parameters for carnitine, creatine, creatinine, carnosine, and anserine concentration in LM and to evaluate their associations with Warner-Bratzler shear force (WBSF) and beef palatability traits. Longissimus muscle samples from 2,285 Angus cattle were obtained and fabricated into steaks for analysis of carnitine, creatine, creatinine, carnosine, anserine, and other nutrients, and for trained sensory panel and WBSF assessments. Restricted maximum likelihood procedures were used to obtain estimates of variance and covariance components under a multiple-trait animal model. Estimates of heritability for carnitine, creatine, creatinine, carnosine, and anserine concentrations in LM from ...


Tol2 Gene Trap Integrations In The Zebrafish Amyloid Precursor Protein Genes Appa And Aplp2 Reveal Accumulation Of Secreted App At The Embryonic Veins, Hsin-Kai Liao, Ying Wang, Kristin E. Watt, Qin Wen, Justin Breitback, Chelsy K. Kemmet, Karl J. Clark, Stephen C. Ekker, Jeffrey J. Essner, Maura Mcgrail Feb 2012

Tol2 Gene Trap Integrations In The Zebrafish Amyloid Precursor Protein Genes Appa And Aplp2 Reveal Accumulation Of Secreted App At The Embryonic Veins, Hsin-Kai Liao, Ying Wang, Kristin E. Watt, Qin Wen, Justin Breitback, Chelsy K. Kemmet, Karl J. Clark, Stephen C. Ekker, Jeffrey J. Essner, Maura Mcgrail

Genetics, Development and Cell Biology Publications

Background—The single spanning transmembrane amyloid precursor protein (APP) and its proteolytic product, amyloid-beta (Aβ) peptide, have been intensely studied due to their role in the pathogenesis of Alzheimer’s disease. However, the biological role of the secreted ectodomain of APP, which is also generated by proteolytic cleavage, is less well understood. Here, we report Tol2 red fluorescent protein (RFP) transposon gene trap integrations in the zebrafish amyloid precursor protein a (appa) and amyloid precursor-like protein 2 (aplp2) genes. The transposon integrations are predicted to disrupt the appa and aplp2 genes to primarily produce secreted ectodomains of the corresponding proteins ...


Structure-Function Analyses Of The Tre1 G Protein-Coupled Receptor, Margaret Marie Pruitt Jan 2012

Structure-Function Analyses Of The Tre1 G Protein-Coupled Receptor, Margaret Marie Pruitt

Graduate Theses and Dissertations

Cell migration is a process that is essential to animals during their entire lifetime and improper cell migration is implicated in diseased states such as cancer. Primordial germ cell migration in Drosophila melanogaster is an excellent model to study cell migration. The movements of Drosophila primordial germ cells are well documented and some of the molecules involved have been identified. The G protein-coupled receptor (GPCR) trapped in endoderm-1 (tre1) is one of the first genes involved in primordial germ cell migration. The work in this dissertation focuses on the GPCR Tre1, the roles of Tre1 in Drosophila germ cell migration ...


New Tools For Studying Mal Gene Regulation In Yersinia Pestis, Carrie Jo Oster Jan 2012

New Tools For Studying Mal Gene Regulation In Yersinia Pestis, Carrie Jo Oster

Graduate Theses and Dissertations

Y. pestis, the etiologic agent of plague, is a significant human pathogen because of its historic role as the cause of three major pandemics and its current role as a potential bioterrorism agent. Gene regulation in this organism is complex and multiple microarray studies have shown that the expression of several genes is affected by quorum sensing as well as temperature. Maltose utilization genes are of particular interest because they are regulated by both of these systems. In order to perform genetic studies on mal genes, and to better understand gene expression in general in Y. pestis, we developed a ...


Mucopolysaccharidosis Type Iiib And Galnac Transferase Double Knockout Mice And Additional Studies In Gene Therapy And Animal Models To Assess Pathogenesis And Therapy, Eman Elsayed Abass Mohammed Jan 2012

Mucopolysaccharidosis Type Iiib And Galnac Transferase Double Knockout Mice And Additional Studies In Gene Therapy And Animal Models To Assess Pathogenesis And Therapy, Eman Elsayed Abass Mohammed

Graduate Theses and Dissertations

The Mucopolysaccharidoses (MPSs) are a class of lysosomal storage diseases characterized by lysosomal accumulation of glycosaminoglycan (GAG). Understanding the pathogenesis of the neuropathic forms of MPS and improving treatment options for these disorders are major areas of research in the laboratory of Dr. Ellinwood. The neuropathic MPSs include MPS I, II, III (A-D), and VII, all of which have primary storage of heparan sulfate (HS), and a secondary accumulation of GM2 and GM3 gangliosides. Clinically patients with the severe forms of these disorders have central nervous system defects. Mucopolysaccharidosis III, also known as Sanfilippo syndrome, has four subtypes designated as ...


Molecular Diagnostic For Boll Weevil (Coleoptera: Curculionidae) Based On Amplification Of Three Species-Specific Microsatellites, Kyung Seok Kim, Zsofia Szendrei, Cesar Rodriguez-Saona, Phillip G. Mulder Jr., Thomas W. Sappington Apr 2009

Molecular Diagnostic For Boll Weevil (Coleoptera: Curculionidae) Based On Amplification Of Three Species-Specific Microsatellites, Kyung Seok Kim, Zsofia Szendrei, Cesar Rodriguez-Saona, Phillip G. Mulder Jr., Thomas W. Sappington

Entomology Publications

The boll weevil, Anthonomus grandis grandis Boheman (Coleoptera: Curculionidae), is a serious pest of cultivated cotton, Gossypium hirsutum L., in the Americas, and reinfestation of zones from which they have been eradicated is of perpetual concern. Extensive arrays of pheromone traps monitor for reintroductions, but occasionally the traps collect nontarget weevils that can be misidentified by scouts. For example, the congeneric pepper weevil,Anthonomus eugenii Cano, and other superficially similar weevils are attracted to components of the boll weevil lure or trap color. Although morphologically distinguishable by trained personnel, the potential for misidentification is compounded when captured weevils are dismembered ...


Metabolic Profiling Of Echinacea Genotypes And A Test Of Alternative Taxonomic Treatments, Lankun Wu, Philip M. Dixon, Basil J. Nikolau, George A. Kraus, Mark P. Widrlechner, Eve S. Wurtele Jan 2009

Metabolic Profiling Of Echinacea Genotypes And A Test Of Alternative Taxonomic Treatments, Lankun Wu, Philip M. Dixon, Basil J. Nikolau, George A. Kraus, Mark P. Widrlechner, Eve S. Wurtele

NCRPIS Publications and Papers

The genus Echinacea is used as an herbal medicine to treat a variety of ailments. To better understand its potential chemical variation, 40 Echinacea accessions encompassing broad geographical and morphological diversity were evaluated under controlled conditions. Metabolites of roots from these accessions were analyzed by HPLC-photo diode array (HPLC-PDA), GC-MS, and multivariate statistical methods. In total, 43 lipophilic metabolites, including 24 unknown compounds, were detected. Weighted principal component analysis (WPCA) and clustering analysis of the levels of these metabolites across Echinacea accessions, based on Canberra distances, allowed us to test two alternative taxonomic treatments of the genus, with the further ...


Pathbinder – Text Empirics And Automatic Extraction Of Biomolecular Interactions, Lifeng Zhang, Daniel Berleant, Jing Ding, Tuan Cao, Eve S. Wurtele Jan 2009

Pathbinder – Text Empirics And Automatic Extraction Of Biomolecular Interactions, Lifeng Zhang, Daniel Berleant, Jing Ding, Tuan Cao, Eve S. Wurtele

Genetics, Development and Cell Biology Presentations, Posters and Proceedings

Motivation

The increasingly large amount of free, online biological text makes automatic interaction extraction correspondingly attractive. Machine learning is one strategy that works by uncovering and using useful properties that are implicit in the text. However these properties are usually not reported in the literature explicitly. By investigating specific properties of biological text passages in this paper, we aim to facilitate an alternative strategy, the use of text empirics, to support mining of biomedical texts for biomolecular interactions. We report on our application of this approach, and also report some empirical findings about an important class of passages. These may ...


The Analysis Of Global Gene Expression Related To Starch, Lipid And Protein Composition , Ling Li Jan 2006

The Analysis Of Global Gene Expression Related To Starch, Lipid And Protein Composition , Ling Li

Retrospective Theses and Dissertations

This dissertation provides a greater understanding of starch metabolism and its regulation by using mutants altered in starch accumulation, together with a combination of approaches such as global gene profiling, promoter::GUS/GFP analyses, and genetics;Starch debranching enzymes are critical for starch accumulation. The temporal and spatial co-expression of ISA1 and ISA2 is consistent with previous evidence that they co-function as a protein complex (ISA1/ISA2) for starch synthesis. The extensive temporal and spatial co-expression of ISA2 and ISA3 suggests they might also form a protein complex, taking part in starch metabolism. ISA3 protein is localized in leaf chloroplast ...


Neuronal Functions Of Ahr-1, The Caenorhabditis Elegans Homolog Of The Aryl Hydrocarbon Receptor , Hongtao Qin Jan 2006

Neuronal Functions Of Ahr-1, The Caenorhabditis Elegans Homolog Of The Aryl Hydrocarbon Receptor , Hongtao Qin

Retrospective Theses and Dissertations

The mammalian aryl hydrocarbon receptor (AHR) mediates the toxic effects of dioxins and related compounds. Toxicological and genetic studies have shown that AHR regulates important development events and physiological functions. Interestingly, AHR homologs are found in most metazoans, and studies of AHR regulation and function in invertebrate model systems may provide insight to the ancient functions of AHR. In this thesis, I analyze the endogenous functions of the ahr-1 gene in the nematode Caenorhabditis elegans. ahr-1:GFP is expressed in a subset of neurons. ahr-1 regulates the development of diverse neuronal cell types, including the AVM and PLM touch receptor ...


Repair Of Endonuclease- And Transposon-Induced Dna Double Strand Breaks By Host Factors In Arabidopsis , Zhuying Li Jan 2006

Repair Of Endonuclease- And Transposon-Induced Dna Double Strand Breaks By Host Factors In Arabidopsis , Zhuying Li

Retrospective Theses and Dissertations

The maize Ac/Ds transposable elements are members of the hAT transposon family. Ac/Ds transpose in diverse species and via a mechanism similar to that involved in V(D)J recombination. Here, we studied the repair of Ds transposition-induced hairpins by host factors in Arabidopsis. The results indicate that AtKu could position hairpin-opening to sites close to the hairpin apex. In the absence of AtMre11 or AtKu70, repair junctions have a higher frequency of small deletions (3-10bp) and larger deletions (11-50bp), respectively. Comparison of the repair of I-SceI- and Ds-induced DSB indicates that Ac transposase could influence Ds-induced DSB ...


Characterization And Interactions Of Tbx2 , Christian Nephi Paxton Jan 2006

Characterization And Interactions Of Tbx2 , Christian Nephi Paxton

Retrospective Theses and Dissertations

T-box (Tbx) proteins are dynamically expressed throughout embryogenesis and appear to have significant roles in a number of developmental processes. The T-box family is characterized by a highly conserved DNA binding domain. Over twenty mammalian T-box proteins have been identified, many of which have the demonstrated ability to regulate transcription. Both activators and repressors of gene transcription have been described within this family, but how their functions are performed is largely unknown;Tbx2 is a powerful transcriptional repressor with functional roles identified in cardiogenesis and limb development. However the functional domain(s) of Tbx2 are poorly characterized. Tbx2 contains a ...


Studies Of Hypoxia Response And Regulation Of Hypoxia-Inducible Factor Hif-1 In Caenorhabditis Elegans , Chuan Shen Jan 2006

Studies Of Hypoxia Response And Regulation Of Hypoxia-Inducible Factor Hif-1 In Caenorhabditis Elegans , Chuan Shen

Retrospective Theses and Dissertations

All aerobic organisms require molecular oxygen to generate metabolic energy for normal growth and survival. During evolution, multi-cellular organisms have developed and refined complex networks for adaptation to hypoxic environments at both systemic and cellular levels. Adaptation to hypoxia largely results from changes in the activity and expression of key proteins. These include proteins involved in increasing oxygen delivery to hypoxic tissues and proteins that facilitate glycolysis for anaerobic metabolism. The mammalian transcription factor hypoxia-inducible factor (HIF) is a master regulator of oxygen homeostasis. More than 100 target genes of HIF mediate broad systemic and local responses to hypoxia, including ...


Molecular Genetic Analyses Of Oilseed Brassica Germplasm: Determination Of Life Forms And Germplasm Management Strategies By Using Microsatellite Markers And Flowering Locus-C (Flc1 And Flc3) Gene Sequences , Von Mark V. Cruz Jan 2006

Molecular Genetic Analyses Of Oilseed Brassica Germplasm: Determination Of Life Forms And Germplasm Management Strategies By Using Microsatellite Markers And Flowering Locus-C (Flc1 And Flc3) Gene Sequences , Von Mark V. Cruz

Retrospective Theses and Dissertations

Molecular markers were utilized in conjunction with phenological and morphological information, and statistical methods for diversity analyses to address plant genetic resource conservation issues. The objectives of this study have been categorized into two sets. The first set pertains to exploratory surveys of genetic variation using molecular markers and DNA sequences to determine if microsatellite markers or variation in two copies of the FLOWERING LOCUS C gene (FLC1 and FLC3) can be used in determining life forms of fifty representative accessions of Brassica napus. The second set of objectives addresses good genebank practices and includes: characterization of flowering time of ...


Plant Genome Informatics: Evaluation And Analysis Of Genomic Dna Features Involved In The Transcriptional Processing Of Protein Coding Genes , Shannon Dwayne Schlueter Jan 2006

Plant Genome Informatics: Evaluation And Analysis Of Genomic Dna Features Involved In The Transcriptional Processing Of Protein Coding Genes , Shannon Dwayne Schlueter

Retrospective Theses and Dissertations

As biological data collection methods have become more cost effective and less time consuming, the necessity for computational tools to store, manage, and analyze this data has led to the creation of a broad field of research. Bioinformatics, while firmly rooted in the technology of information management, is now a mainstream component in the majority of scientific investigations. With the vast majority of effort in bioinformatics being applied to research on vertebrate species, researchers in the plant sciences have often been left with less than satisfactory tools. The research presented in this dissertation was done in an effort to advance ...


Recognition And Signal Transduction In The Expression Of Phytophthora Resistance In Soybean [Glycine Max (L) Merr] , Hongyu Gao Jan 2006

Recognition And Signal Transduction In The Expression Of Phytophthora Resistance In Soybean [Glycine Max (L) Merr] , Hongyu Gao

Retrospective Theses and Dissertations

Phytophthora sojae, a hemibiotrophic oomycete, is the causal pathogen of stem and root rot disease of soybean (Glycine max [L.] Merr). The annual soybean yield loss suffered from this disease is valued at about 300 million dollars. A series of single Rps genes protect soybean from the root and stem rot disease. In the last two decades Rps1-k has been the most stable and widely used Phytophthora resistance gene in the major soybean-producing regions of the United States;Two classes of CC-NB-LRR-type Rps1-k genes were isolated from the Rps1-k locus. Representative members from each gene class were expressed in transgenic ...