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Full-Text Articles in Molecular Biology

Mirnas Collaborate With A Conserved Rna Binding Protein To Ensure Development And Stress Response In C. Elegans, Rebecca A. Zabinsky Jan 2015

Mirnas Collaborate With A Conserved Rna Binding Protein To Ensure Development And Stress Response In C. Elegans, Rebecca A. Zabinsky

Molecular, Cellular, and Developmental Biology Graduate Theses & Dissertations

miRNAs play critical roles in development and other cellular processes in C. elegans even though most individual miRNAs are not essential for development or viability (Miska et al., 2007). Extensive studies in the field have suggested that most miRNA functions are executed through complex miRNA-target interaction networks. Furthermore, such networks may also function semi-redundantly with other regulatory systems to shape gene expression dynamics for proper physiological functions. Hypothesizing that miRNAs function in stress conditions, I collaborated with a postdoctoral fellow to investigate the role of a specific miRNA miR-71 in starvation induced L1 diapause. Hypothesizing that miRNAs collaborate with other ...


Effect Of Mirna-138 Injected Into The Medial Habenula On Nicotine Intake And Preference, Kelsey Fitzgerald Jan 2015

Effect Of Mirna-138 Injected Into The Medial Habenula On Nicotine Intake And Preference, Kelsey Fitzgerald

Undergraduate Honors Theses

Nicotine is one of the most commonly abused drugs in the world today and is associated with many adverse health outcomes, including death. Nicotine binds to nicotinic acetylcholine receptors (nAChRs) in the brain reward pathway, triggering the release of a number of neurotransmitters, including dopamine. The goal of the current study was to determine whether miR-138, a miRNA that targets and reduces β4 subunit gene expression, injected into the medial habenula would lead to changes in nicotine intake/preference. We hypothesized that miR-138 would down regulate β4 expression, leading to decreased aversion to nicotine and increased nicotine intake/preference. Male ...


Genomic Analysis Of Micrornas In Mouse Skin: Quantification, Biogenesis, Target Recognition And Regulatory Functions, Zhaojie Zhang Jan 2013

Genomic Analysis Of Micrornas In Mouse Skin: Quantification, Biogenesis, Target Recognition And Regulatory Functions, Zhaojie Zhang

Molecular, Cellular, and Developmental Biology Graduate Theses & Dissertations

MiRNAs are a class of small RNAs, approximately 20~22 nucleotides in length. These broadly conserved molecules represent a novel layer of gene regulation that fine-tunes the output of the transcriptome and contributes to the robustness of biological pathways. However, the quantitative picture of the miRNA pathway is unclear. Fundamental questions concerning the biogenesis pathway, target recognition and regulatory functions of individual miRNAs within mammalian tissues remain poorly understood. In this thesis, I employ mouse skin as a model system to examine the miRNA pathway with genomic approaches.

I profiled the expression of miRNAs in mouse skin with a small ...


Bmp Signaling Goes Posttranscriptional In A Microrna Sort Of Way, Catherine A. Reinke, Richard W. Carthew Jan 2008

Bmp Signaling Goes Posttranscriptional In A Microrna Sort Of Way, Catherine A. Reinke, Richard W. Carthew

Faculty Publications

Aberrant microRNA (miRNA) expression correlates with human diseases such as cardiac disorders and cancer. Treatment of such disorders using miRNA-targeted therapeutics requires a thorough understanding of miRNA regulation in vivo. A recent paper in Nature by Davis et al. expands our understanding of miRNA biogenesis and maturation, elucidating a mechanism by which extracellular signaling directs cell differentiation via posttranscriptional regulation of miRNA expression.